Colloidal gold nanoparticles (AuNPs) are critical for chemical sensing and therapeutic applications, necessitating effective surface functionalization for stability in biological environments.
Traditional methods use thiol groups for bonding, but these can be complex and not resilient to certain conditions, leading to the exploration of alkyne derivatives as a preferable alternative.
The study shows that alkyne derivatives provide easier preparation, higher stability, and better conjugation efficiency, and highlights their effectiveness in detecting hydrogen peroxide and streptavidin proteins.