[Assessment of malaria knowledge and capability on Plasmodium detection among professionals from class III malaria-endemic counties of Henan Province in 2012].

Objective: To evaluate the capability of malaria parasite detection among professionals from Class III malaria endemic counties of Henan Province in 2012. METHEDS: The capacity assessment of professionals from the Centres for Disease Control and the medical institutions was done in September to December 2012. The content of the assessment included malaria knowledge (including malaria etiology, clinical manifestation, diagnosis, treatment and epidemiology and so on, 100 scores as full marks and 60 as passing score), making blood slides of Plasmodium (4 slides in 1 hour, including Giemsa staining, 40 scores as full marks and 24 as passing score) and identification of species with microscopy (6 slides, 8 min per slide 60 scores as full marks and 36 as passing score).

[Polymorphism analysis on the genotypes of circumsporozoite protein of Plasmodium vivax with PRC-RFLP].

Thirty-seven blood samples from Plasmodium vivax-infected patients were collected in Henan Province in 2011. The gene for circumsporozoite protein (PvCSP) was amplified by nested PCR. According to the epidemiological data, among the 37 Plasmodium vivax malaria cases, 26 were indigenous cases and 11 were imported cases.

[Laboratory detection on two cases with imported Plasmodium ovale infection].

Objective: To compare the laboratory tests of the imported Plasmodium ovale infection and analyse the genetic character.

Methods: After Giemsa staining and microscopy, CareStart rapid detection and nested PCR were used to detect two cases with P. ovale infection returning from Congo (Brazzaville) in Henan Province.

[Epidemiological analysis of imported malaria in Henan Province in 2011].

Objective: To analyze the epidemiological characteristics of imported malaria in Henan Province in 2011, and provide scientific basis for the control of imported malaria.

Methods: Data of imported malaria were collected and analyzed by SPSS11.5 in 2011.

[Molecular identification of Anopheles hyrcanus complex by using single enzyme digestion PCR-RFLP method].

Objective: To establish a novel molecular identification method for discrimination of members within Anopheles hyrcanus complex.

Methods: The sequences of the ribosomal DNA second internal transcribed spacer (rDNA ITS2) region of An. hyrcanus complex, including An.

Vector capacity of Anopheles sinensis in malaria outbreak areas of central China.

Background: Both falciparum and vivax malaria were historically prevalent in China with high incidence. With the control efforts, the annual incidence in the whole country has reduced to 0.0001% except in some areas in the southern borders after 2000.

[Imported falciparum malaria situation in Henan Province during 2005-2009].

Imported falciparum malaria data of Henan Province in 2005-2009 were collected and statistically analyzed by using Microsoft Office Excel 2003. A total of 84 imported falciparum malaria cases with one death were reported during 2005-2009. The ratio of males to females was 20 : 1(80/4).

[Evaluation on malaria situation in areas along Yellow River and Huaihe River by indirect fluorescent antibody test].

Objective: To evaluate malaria situation by indirect fluorescent antibody test (IFAT) in 12 villages of six counties along Yellow River and Huihe River in Anhui, Henan and Hubei Provinces.

Methods: Cross sectional seroepidemiologic survey was conducted through IFAT in Anopheles sinensis area and A. anthropophagus plus A.

[Impact of blastocystis hominis infection on ultrastructure of intestinal mucosa in mice].

Objective: To observe the ultrastructural change ot intestinal mucosa in mice infected with Blastocystis hominis, and to study the pathogenic mechanism of B. hominis infection.

Methods: 20 Kunming mice were randomly divided into 4 groups: group A treated with immunosuppressant (dexamethasone), group B without immunosuppressant, group C as normal control and group D as immunosuppressant control.

Malaria control in Henan Province, People's Republic of China.

We analyzed malaria prevalence and evaluated the effect of malaria control measures in Henan Province, People's Republic of China between 1993 and 2004. Data relating to malaria epidemics, malaria control measures and their effects, and vector surveillance between 1993 and 2004 were collected and analyzed. Mean malaria incidence during this period was 2.

[Study on number and mature rate of eggs in gravid proglottids of Taenia solium].

Objective: To investigate the number and mature rate of eggs in gravid proglottids of Taenia solium.

Methods: Ten worms of Taenia solium, expelled from patients, were detected. Eggs were collected from the last 10 gravid proglottids of each worm.