[Polymorphism of the Full-Length mRNA Sequences of MNS blood group-related genes , and ].

Objective: The characteristics of the full-length mRNA sequences of MNS blood group-related genes , and were analyzed to understand the polymorphism of MNS blood group genes.

Methods: Anticoagulated blood within 24 h from 500 unpaid blood donors (8 ml each) were randomly selected, and MN, Ss and Mia blood types were identified by serological methods. 5 samples with different combinations of MNS and Mia blood types were randomly selected from 500 samples, and peripheral blood mononuclear cells (PBMC) were isolated by density gradient centrifugation, then total mRNA was extracted.