Main Pathways and Ion Channels Differentially Expressed in the Transcriptome of Male and Female Adult Using a Deep Sequencing Approach.

Background: The adult stage is an important period in the life cycle of , as it is at this stage that male and female worms produce thousands of fertilized eggs daily.

Methods: To explore the transcriptional details of adult male and female , three groups of male and female adult worms were collected, and their transcriptome profiles were analyzed using an Illumina next-generation sequencing platform. A total of 283,910,174 clean reads were obtained, and 137,626 unigenes and 237,059 transcripts were then generated.

Next-Generation Sequencing Reveals That Oxidative Phosphorylation Might Be a Key Pathway Differently Expressed in the Third and Fourth Stages Larvae of .

Background: When develops from the third and fourth stage, it needs to change its host from the middle host, snail to the final host, rat. However, the mechanism involved in this change remains unclear.

Methods: The transcriptome differences of the third and fourth stages of were explored by next-generation Illumina Hiseq/Miseq sequencing in China, in 2018.

Value of promoter methylation in colorectal cancer screening and prognosis assessment and its influence on the activity of cancer cells.

Introduction: The aim of the study was to investigate the effect of promoter methylation on the proliferative, invasive and migration potential of colorectal cancer cells, including its potential use for the early detection and prognostic assessment of colorectal cancer.

Material And Methods: Quantitative methylation-specific PCR (qMSP) was used to detect the methylation status of the promoter region in peripheral blood samples drawn from patients with colorectal adenocarcinoma, benign colorectal adenoma, and matched healthy controls. Putative CpG methylation sites were then pyrosequenced.

[A bite of parasite, experiences of application of documentaries into parasitology class teaching].

Medical parasitology education has been facing some difficulties, because it is a course of wide range, lacking clinical cases and concerned specimens of parasites currently. In addition, its relationship with life is not closely enough. All these reasons may impact the effect of class education negatively.

Inhibition of Nonsmall Cell Lung Cancer Cell Migration by Protein Arginine Methyltransferase 1-small Hairpin RNA Through Inhibiting Epithelial-mesenchymal Transition, Extracellular Matrix Degradation, and Src Phosphorylation In Vitro.

Background: Protein arginine methyltransferases 1 (PRMT1) is over-expressed in a variety of cancers, including lung cancer, and is correlated with a poor prognosis of tumor development. This study aimed to investigate the role of PRMT1 in nonsmall cell lung cancer (NSCLC) migration in vitro.

Methods: In this study, PRMT1 expression in the NSCLC cell line A549 was silenced using lentiviral vector-mediated short hairpin RNAs.

Seroprevalence of Toxoplasma gondii infection in dairy goats in Shaanxi Province, Northwestern China.

Background: Toxoplasma gondii is an important zoonotic pathogen causing significant human and animal health problems. Infection in dairy goats not only results in significant reproductive losses, but also represents an important source of human infection due to consumption of infected meat and milk. In the present study we report for the first time seroprevalence of T.