Analysis of entire hepatitis B virus genomes reveals reversion of mutations to wild type in natural infection, a 15 year follow-up study.

It has been reported that some mutations in the genome of hepatitis B virus (HBV) may predict the outcome of the virus infection. However, evolutionary data derived from long-term longitudinal analysis of entire HBV genomes using next generation sequencing (NGS) remain rare. In this study, serum samples were collected from asymptomatic hepatitis B surface antigen (HBsAg) carriers from a long-term prospective cohort.

High prevalence of Clonorchis sinensis infection in Guangxi, Southern China.

Background: Soil-transmitted helminths (STHs), such as hookworm, roundworm and whipworm, and food-borne trematodiases, including Clonorchis sinensis, remain a public health problem worldwide, especially in tropical and subtropical regions.

Objective: We aimed to determine the current prevalence of these parasites in Guangxi, China, which is located in a subtropical region.

Methods: A cross-sectional study and a 4-year longitudinal surveillance study were carried out.

[Investigation on infections in marketed cats in Nanning City].

Objective: To understand infections in cats in Nanning City, so as to provide evidence for the control of the reservoir host of .

Methods: The cat livers were purchased from cat slaughterhouses in Nanning City. The cat gallbladder and liver were dissected, and liver flukes were collected and counted.

TLR2 signal influences the iNOS/NO responses and worm development in C57BL/6J mice infected with Clonorchis sinensis.

Background: Although the responses of inducible nitric oxide synthase (iNOS) and associated cytokine after Clonorchis sinensis infection have been studied recently, their mechanisms remain incompletely understood. In this study, we investigated the effects of toll-like receptor 2 (TLR2) signals on iNOS/nitric oxide (NO) responses after C. sinensis infection.

[Establishment of Duplex PCR for Identifying Metagonimus yokogawai and Haplorchis taichui].

Objective: To develop a duplex PCR method for identifying Metagonimus yokogawai and Haplorchis taichui.

Methods: ITS1 sequences of M. yokogawai and H.

[Ultrastructural observation on nymphal Armillifer sp. by scanning electron microscopy and phylogenetic analysis based on 18S rRNA].

Objective: To observe the ultrastructure of nymphal Armillifer sp. isolated from Macaca fascicularis by using scanning electron microscope (SEM), and analyze the phylogenetic relationships based on 18S rRNA gene sequences.

Methods: The parasite samples stored in 70% alcohol were fixed by glutaraldehyde and osmium peroxide.