Publications by authors named "Yun-Kang Yang"
Article Synopsis
- Two surgical techniques, the separate vertical wire (SVW) and improved candy box (CB), are proposed for treating inferior pole patellar fractures, but the proper wire placement remains unclear.
- Finite element analysis was used to simulate different wire positions and loads, revealing that specific placements minimize stress and displacement on the patella and wire.
- Findings suggest that the SVW technique benefits from positioning the wire near the fracture's end, while the CB technique is optimal with the wire near the upper posterior aspect of the patella, but further studies are needed for confirmation.
Objectives: In the present study, it was examined whether polymorphism of circadian locomotor output cycle kaput (CLOCK) gene 3111T/C was associated with susceptibility of Alzheimer disease (AD).
Methods: This study was conducted using a case-control method. Genotypes of apolipoprotein E (APOE e4) and CLOCK gene 3111T/C were determined by restriction fragment length polymorphism method and TaqMan assay method, respectively.
Objective: To explore the feasibility for therapy of spinal cord injury (SCI) by genetic engineering neural stem cell (NSC) modified by lentiviral vector.
Methods: Following the construction of the genetic engineering NSC modified by lentivirus to secrete both neurotrophic factor-3 (NT-3) and green fluorescence protein (GFP), hemisection of spinal cord at the level of T10 was performed in 56 adult Wistar rats that were randomly divided into 4 groups (n = 14), namely 3 therapeutic groups and 1 control group. The therapeutic groups were dealed with NSC, genetic engineering NSC, and concentrated lentiviral supernatant which carries both GFP and NT-3, respectively.
Objective: To explore the feasibility to construct genetic engineering human neural stem cells (hNSCs) mediated by lentivirus to express multigene in order to provide a graft source for further studies of spinal cord injury (SCI).
Methods: Human neural stem cells from the brain cortex of human abortus were isolated and cultured, then gene was modified by lentivirus to express both green fluorescence protein (GFP) and rat neurotrophin-3 (NT-3); the transgenic expression was detected by the methods of fluorescence microscope, dorsal root ganglion of fetal rats and slot blot.
Results: Genetic engineering hNSCs were successfully constructed.