Production of polyclonal antibody against recombinant ORF 112 L of rock bream (Oplegnathus fasciatus) iridovirus (RBIV) and in vitro neutralization.

Rock bream iridovirus (RBIV) is a causative agent of epizootics among cultured rock bream (Oplegnathus fasciatus) in Korea. In this study, the immunogenic property of ankyrin repeats gene (ORF 112L) from RBIV was evaluated to develop vaccines against RBIV. ORF 112L of RBIV was cloned into expression vector of pGEX-4T-1.

Neutralization of white spot syndrome virus (WSSV) for Penaeus chinensis by antiserum raised against recombinant VP19.

This study was carried out to neutralize the WSSV one of the most virulent pathogen causing large economic damage in shrimp culture industry using the antiserum produced against recombinant WSSV envelope protein VP19 (rVP19) as a tool to evaluate WSSV infection mechanism. A fragment of VP19 was expressed in Sf21 insect cell using baculovirus expression system as fusion protein with 6 His-tag. Then, polyclonal antiserum against rVP19 was raised in white rabbit.

Immobilization of cryopreserved primary rat hepatocytes for the development of a bioartificial liver system.

Primary rat hepatocytes were cryopreserved in hormonally-defined medium (HDM) containing 40% (v/v) fetal bovine serum (FBS) and 10% (v/v) dimethyl sulfoxide (DMSO) in liquid N2 for 6 months. After thawing, the cells were immobilized using 2% (w/v) alginate and 0.5% (w/v) chitosan solutions.