An enzyme-linked immunosorbent assay for monoester-type aconitic alkaloids and its application in the pharmacokinetic study of benzoylhypaconine in rats.

A new enzyme-linked immunosorbent assay (ELISA) method for quantitative determination of monoester-type aconitic alkaloids was developed. The antibodies derived from the immunogen of benzoylmesaconine (BM) could be electively affined to benzoylaconitine-type alkaloids with an ester bond (14-benzoyl-), especially to benzoylhypaconine (BH, 140.02% of cross-reactivity).

Studies on the flavone glycosides from Fructus Kochiae.

A series of flavone glycosides were isolated from Fructus Kochiae for the first time, including two new flavone glycosides. The structures were established by interpretation of their spectroscopic data. Two new flavone glycosides are quercetin 3-O-β-d-apiofuranosyl-(1 → 2)-β-d-galactopyranosyl-7-O-β-d-glucopyranoside (1) and quercetin 3-O-α-l-rhamnopyranosyl-(1 → 6)-β-d-galactopyranosyl-7-O-β-d-sophoroside (2).

[The effect of different intensity aerobic exercise training on blood pressure control in hypertensive patients.].

Objective: To compare the therapeutic effect of aerobic exercise training of different intensity on hypertension.

Methods: Sixty hypertensive patients were randomly allocated to 2 groups and treated with aerobic exercise training at 20% or 60% of maximal intensity. Not only were the relevant parameters of exercise capacity measured by the use of treadmill exercise test, but also was ambulatory blood pressure monitored and the quality of life recorded before and after the therapy.