The Use of PET Imaging for Prognostic Integrin αβ Phenotyping to Detect Non-Small Cell Lung Cancer and Monitor Drug Resistance Responses.

Purpose: Growing evidence has demonstrated that aberrant expression of integrin α2β1 might contribute to the invasion, metastasis and drug resistance of non-small cell lung cancer (NSCLC). Thus, the integrin α2β1 targeting Ga-DOTA-A2B1 tracer was validated in NSCLC in contrast to accumulation of the clinically used F-FDG PET tracer to see if Ga-DOTA-A2B1-PET imaging can offer a valuable and critical diagnostic imaging criterion for the identification of phenotypes of aggressive lung cancer.

Methods: To verify the prognostic value of integrin α2β1, several quantitative and functional assays were validated in different NSCLC cell lines (CL1-0, CL1-5, A549 and selected A549 cells).

[Identification of plantaginis semen based on ITS2 and psbA-trnH sequences].

In order to evaluate the efficiency of ITS2 and psbA-trnH sequences used as DNA barcodes to distinguish Plantaginis Semen from its adulterants, we collected 71 samples of Plantaginis Semen and its adulterants. The ITS2 and psbA-trnH sequences were aligned through Clustal W, and the genetic distances were calculated by kimura 2-parameter (K2P) model and the Neighbor-Joining (NJ) phylogenetic trees were constructed using MEGA 5.1.

[Identification of pyrrosiae folium and its adulterants based on psbA-trnH sequence].

In this study, the psbA-trnH sequence as DNA barcode was used to evaluate the accuracy and stability for identification pteridophyte medicinal material Pyrrosiae Foliumas from adulterants. Genomic DNA from 106 samples were extracted successfully. The Kimura 2-Parameter (K2P) distances and ML tree were calculated using software MEGA 6.

A rat model of thrombosis in common carotid artery induced by implantable wireless light-emitting diode device.

This work has developed a novel approach to form common carotid artery (CCA) thrombus in rats with a wireless implantable light-emitting diode (LED) device. The device mainly consists of an external controller and an internal LED assembly. The controller was responsible for wirelessly transmitting electrical power.

[Study on extraction and purification technology of Hubei ophiopogon saponins].

Objective: To explore the extraction and purification technology of total saponins from the effective parts of Liriope spicata.

Methods: Orthogonal design was used. Macroporous resin was selected to separate and purify total saponin from the effective parts of Liriope spicata.

Differences between chondrocytes and bone marrow-derived chondrogenic cells.

Implantation of autologous chondrogenic cells has become the mainstay strategy for repairing articular cartilage defects. Because the availability of autologous chondrocytes is extremely limited, many recent studies have used artificially induced mesenchymal stem cells (iMSCs) as substitutes for chondrocytes. In this study, we analyzed the differences between the iMSCs and chondrocytes, including their molecular biological and mechanical properties.

Significance of the pH-induced conformational changes in the structure of C-reactive protein measured by dual polarization interferometry.

Emerging evidence indicates that the conformation of C-reactive protein (CRP) plays important roles in human inflammation and cardiovascular disease (CVD). The different conformations in the structure of CRP under different pH conditions remain an important issue to be investigated for explaining various functions of CRP under certain physiologic and pathologic conditions. We directly measured the pH-induced conformational changes in the structure of CRP by dual polarization interferometry (DPI).

Mass-size distributions of particulate sulfate, nitrate, and ammonium in a particulate matter nonattainment region in southern Taiwan.

Concentrations and distributions of three major water-soluble ion species (sulfate, nitrate, and ammonium) contained in ambient particles were measured at three sampling sites in the Kao-ping ambient air quality basin, Taiwan. Ambient particulate matter (PM) samples were collected in a Micro-orifice Uniform Deposit Impactor from February to July 2003 and were analyzed for water-soluble ion species with an ion chromatograph. The PM1/ PM2.