Publications by authors named "Yun-Guo Qian"
Article Synopsis
- The use of gene expression markers like EGFP has been important for selecting mammalian cell clones that produce high levels of recombinant proteins, but more cost-effective methods are still needed.
- A new method is introduced that co-expresses EGFP and a target protein linked by an internal ribosome entry site, enabling efficient clonal selection in standard 96-well plates.
- This technique was tested by expressing recombinant porcine β-defensin 1 in Marc 145 cells, showing its effectiveness as an alternative to traditional cell sorting methods for identifying high-producer clones.
Survival and engraftment of mouse embryonic stem cells in the mammary gland.
In Vitro Cell Dev Biol Anim
March 2011
Embryonic stem (ES) cells have been investigated in many animal models of severe injury and degenerative disease. However, few studies have examined the ability of ES cells to improve functional outcome following mammary gland injury. This study investigates the feasibility of implanting mouse ES cells labeled with enhanced green fluorescence protein in the developing mammary glands in order to acquire lineage-committed cells in mammary (mammary gland epithelial cell or luminal cell).
View Article and Find Full Text PDFES (embryonic stem)-derived cells have been investigated in many animal models of severe injury and degenerative disease. However, few studies have examined the ability of ES-derived cells to improve functional outcome following partially damaged breast and also the modification of mammary tissue to produce costly proteins. This study investigates the feasibility of implanting mES-dK (mouse ES-derived keratinocytes-like) cells stably transfected with a mammary gland special expression vector for the PBD-1 (porcine beta-defensin 1) in developing mammary glands.
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