[Effects of inhibition of Rho/ROCK pathway on proliferation and migration of airway smooth muscle cells and related mechanisms].

Objectives: To investigate the effects and molecular mechanisms of inhibition of the Ras homolog gene (Rho)/Rho-associated coiled-coil forming protein kinase (ROCK) pathway on the proliferation and migration of airway smooth muscle cells involving myocardin (MYOCD).

Methods: Human airway smooth muscle cells were infected with the adenoviral vector Ad-ZsGreen-shRNA-hROCK1 . The cells were randomly divided into four groups: gene silencing control (shNC) group, shNC + arachidonic acid (AA, Rho/ROCK pathway activator) group, gene silencing (shROCK1) group, and shROCK1 + AA group (=3 each).