Development of Haploid Plants by Shed-Microspore Culture in (Jacq.) A. DC.

Anther and microspore cultures are efficient methods for inducing haploids in plants. The microspore culture by chromosome-doubling method can produce double haploid lines, developing pure lines within the first or second generations. This study aimed to induce haploid plants in using the shed-microspore culture method.

Inhibitory Activity of Natural -Khellactone on Soluble Epoxide Hydrolase and Proinflammatory Cytokine Production in Lipopolysaccharides-Stimulated RAW264.7 Cells.

The pursuit of anti-inflammatory agents has led to intensive research on the inhibition of soluble epoxide hydrolase (sEH) and cytokine production using medicinal plants. In this study, we evaluated the efficacy of -khellactone, a compound isolated for the first time from the roots of . The compound was found to be a competitive inhibitor of sEH, exhibiting an IC value of 3.

Inhibitory activity of lignanamides isolated from hemp seed hulls( L.) against soluble epoxide hydrolase.

Soluble epoxide hydrolase (sEH) is a therapeutic target for inflammation. In the present study, we isolated one new () and four known (-) compounds from the ethyl acetate fraction of hemp seed hulls. Their structures were elucidated as lignanamides via nuclear magnetic resonance and mass spectral analyses.

Inhibition by components of of 3CLpro and HCoV-OC43 proliferation.

Coronavirus disease 2019 (COVID-19) is an infectious disease caused by severe acute respiratory syndrome coronavirus-2 (SARS-CoV-2). 3CLpro is a key enzyme in coronavirus proliferation and a treatment target for COVID-19. and , compounds - from had inhibitory activity and binding affinity for 3CLpro.

Inhibition of Soluble Epoxide Hydrolase Activity by Components of .

Soluble epoxide hydrolase (sEH) is a target enzyme for the treatment of inflammation and cardiovascular disease. A extract exhibited ~50% inhibition of sEH at 100 μg/mL, and column chromatography yielded compounds -. Inhibitors , -, , and were non-competitive; inhibitors , , and were competitive.

Inhibitory Activity of 4--Benzoyl-3'--(OMethylsinapoyl) Sucrose from on -Glucuronidase.

Bacterial -glucuronidase in the intestine is involved in the conversion of 7-ethyl-10- hydroxycamptochecin glucuronide (derived from irinotecan) to 7-ethyl-10-hydroxycamptothecin, which causes intestinal bleeding and diarrhea (side effects of anti-cancer drugs). Twelve compounds (1-12) from were evaluated in terms of -glucuronidase inhibition in vitro. 4--Benzoyl-3'-O-(O-methylsinapoyl) sucrose (C3) was highly inhibitory at low concentrations.

QTL Mapping for Gummy Stem Blight Resistance in Watermelon ( spp.).

Watermelon () is an economically important fruit crop worldwide. Gummy stem blight (GSB) is one of the most damaging diseases encountered during watermelon cultivation. In the present study, we identified quantitative trait loci (QTLs) associated with GSB resistance in an F population derived from a cross between maternal-susceptible line '920533' () and the paternal-resistant line 'PI 189225' ().

Cloning of genes differentially expressed during the initial stage of fruit development in melon (Cucumis melo cv. Reticulatus).

We have cloned genes involved in the initial stage of fruit development in the melon by suppression subtractive hybridization. A cDNA library of unfertilized ovules was subtracted from that of fruit 9 days after pollination (DAP); 10 of the 40 selected cDNA clones were identified by reverse Northern analysis as genes differentially expressed in fruit at 9 DAP. Seven of the ten genes were homologous to genes of known function; two were related to genes with unknown functions, and one was novel.