Multi-bioapplicable fluorescence chemosensor for sequentially identifying gallium ion and pyrophosphate: Application to environmental samples, human serum, zebrafish and celery.

A multi-bioapplicable fluorescence chemosensor MQV, ((E)-2-methoxy-6-((2-(quinolin-2-yl)hydrazineylidene)methyl)phenol), was developed for sequential detection of Ga and pyrophosphate (PPi) ions. The addition of Ga and PPi to MQV in sequence strongly caused an off-on-off fluorescent response in PBS buffer/DMSO (8:2, v/v, pH 7.4).

Enhanced production of ectoine from methane using metabolically engineered Methylomicrobium alcaliphilum 20Z.

Background: Ectoine (1,3,4,5-tetrahydro-2-methyl-4-pyrimidinecarboxylic acid) is an attractive compatible solute because of its wide industrial applications. Previous studies on the microbial production of ectoine have focused on sugar fermentation. Alternatively, methane can be used as an inexpensive and abundant resource for ectoine production by using the halophilic methanotroph, Methylomicrobium alcaliphilum 20Z.

Resistance mechanisms and reprogramming of microorganisms for efficient biorefinery under multiple environmental stresses.

In the fermentation process of biorefinery, industrial strains are normally subjected to adverse environmental stresses, which leads to their slow growth, yield decline, a substantial increase in energy consumption, and other negative consequences, which ultimately seriously hamper the development of biorefinery. How to minimize the impact of stress on microorganisms is of great significance. This review not only reveals the damaging effects of different environmental stresses on microbial strains but also introduces commonly used strategies to improve microbial tolerance, including adaptive evolution, reprogramming of the industrial host based on genetic circuits, global transcription machinery engineering (gTME) and bioprocess integration.