[Effects of Sophora japonica extract on alveolar bone mass in ovariectomized osteoporosis mice].

Purpose: To investigate the effect of Sophora japonica extract on alveolar bone mass in ovariectomized osteoporosis mice.

Methods: Six-week-old female non-pregnant wild-type C57BL/6J mice were randomly divided into sham operation group, ovariectomy(OVX) group and OVX+Sophora japonica extract group. Ovaries of the mice in the OVX group and the OVX+Sophora japonica extract group were removed, and the mice in the OVX+Sophora japonica extract group were treated by Sophora japonica extract at a dose of 150 mg/kg, three times a week for 4 weeks; while mice of the other two groups were given an equal volume of normal saline at the same time.

Rational design and synthesis of triazene-amonafide derivatives as novel potential antitumor agents causing oxidative damage towards DNA through intercalation mode.

In this work, we rationally designed and synthesized two novel triazene-amonafide derivatives 2-(2-(diisopropylamino)ethyl)-5-(3,3-dimethyltriaz-1-en-1-yl)-1H-benzo[de]isoquinoline-1,3(2H)-dione (D-11) and 5-(3,3-diethyltriaz-1-en-1-yl)-2-(2-(diisopropylamino)ethyl)-1H-benzo[de]isoquinoline-1,3(2H)-dione (D-12) as potential antitumor agents. The DNA damage induced by the intercalation mode of D-11 (D-12) towards DNA was electrochemically detected through the construction of efficient biosensors. The consecutive processes of reversible redox of naphthylimide ring and irreversible oxidation of triazene moiety were elucidated on the surface of glassy carbon electrode (GCE) by CV, SWV, and DPV methods.

Age at first birth is associated with the likelihood of frailty in middle-aged and older women: A population-based analysis from NHANES 1999-2018.

Objectives: This study examined whether age at first birth (AFB) is associated with the prevalence of frailty in middle-aged and older women.

Methods: The study included 10,828 women (age ≥ 45 years) from the National Health and Nutrition Examination Survey (NHANES) (1999-2018) in the United States. AFB data were collected using a standardized reproductive health questionnaire.

Prediction of Long-Term Survival Outcome by Lymph Node Ratio in Patients of Parotid Gland Cancer: A Retrospective study.

Background: Lymph node ratio (LNR) has been reported to reliably predict cancer-specific survival (CSS) in parotid gland cancer (PGC). Our study was designed to validate the significance of LNR in patients with PGC.

Methods: Patients diagnosed with stage I-IV PGC were enrolled from Surveillance Epidemiology and End Results database (SEER,  = 3529), which is the training group, and Sun Yat-sen University Cancer Center database (SYSUCC,  = 99), the validation group.

Synthesis of functional dicationic ionic liquids for the treatment of lignocellulosic biomass.

Mono-cationic ionic liquids (MILs) have been widely utilized for the treatment of lignocelluloses. However, the hydrophilic property of MILs increases the difficulty of isolating sugars and their reuse, which limits their industrial application. In the present study, a series of dicationic ionic liquids (DILs) were synthesized with totally different di-cations and di-anions.

Synthesis of novel functional ionic liquids and their application in biomass.

A series of dicationic ionic liquids (ILs) including [PF][(PYR)C(MIM)][Cl], [PF][(PYR)C(PYR)][Cl], [PF][(PYR)C(MIM)][Cl], and [PF][(PYR)C(PYR)][Cl], and monocationic ILs including [(PYR)CCl][PF], [(PYR)CCl][PF], [(MIM)CCOOH][PF] and [(PYR)CCOOH][PF] were synthesized. Their thermal stability and melting points were determined. Their solubility with organic solvents and the miscibility with water were investigated.

Screening of potential IL-tolerant cellulases and their efficient saccharification of IL-pretreated lignocelluloses.

Lignocellulosic biomass as one of the most abundant and renewable resources has great potential for biofuel production. The complete conversion of biomass to biofuel is achieved through the effective pretreatment process and the following enzyme saccharification. Ionic liquids (ILs) are considered as a green solvent for lignocellulose pretreatment.

[Role of endoplasmic reticulum stress-induced apoptosis of trophoblasts in intrahepatic cholestasis during pregnancy].

Objective: To investigate the role of endoplasmic reticulum stress (ERS)-induced trophoblast apoptosis in the development of intrahepatic cholestasis during pregnancy (ICP).

Methods: Twenty pregnant women with ICP and 20 normal pregnant women undergoing cesarean section were enrolled in this study. The number of placenta syncytial knots in these women was determined using HE staining.

[Expectant therapy versus curettage for retained products of conception after second trimester termination of pregnancy: analysis of outcomes and complications].

Objective: To evaluate the prognosis and complications of expectant therapy and curettage for retained product of conception (RPOC) after second trimester termination of pregnancy (TOP).

Methods: A total of 270 patients with RPOC following second trimester TOP in Nanfang Hospital between January, 2014 and December, 2015 were included in this study. The duration of vaginal bleeding time and menstruation recovery interval were compared between patients receiving expectant therapy and curettage for RPOC, and binary logistic regression was used to assess the risk factors for complications in bivariate and multivariate analyses.

Preterm Birth and Subsequent Risk of Acute Childhood Leukemia: a Meta-Analysis of Observational Studies.

Background: Preterm birth (PTB) has been recognized as a crucial long term risk factor for multiple non-communicable diseases. However, studies between the relationship of PTB and the risk of acute childhood leukemia have yielded inconclusive results. Therefore, we performed a meta-analysis to systematically review the current literature to investigate whether PTB is associated with increased risk of acute childhood leukemia.

Maternal HCV infection is associated with intrauterine fetal growth disturbance: A meta-analysis of observational studies.

Since the evidence regarding the association between maternal hepatitis C virus (HCV) infection and impaired intrauterine fetal growth had not been conclusive, the aim of the present study was to evaluate the risk of maternal HCV infection in association with intrauterine fetal growth restriction (IUGR) and/or low birth weight infants (LBW). We performed an extensive literature search of PubMed, MEDLINE, and EMBASE through December 1, 2015. The odds ratios (ORs) of HCV infection and IUGR/LBW were calculated and reported with 95% confidence intervals (95% CIs).

Can HPV vaccine have other health benefits more than cancer prevention? A systematic review of association between cervical HPV infection and preterm birth.

Although the association between high-risk human papillomavirus (HPV) infection and cervical dysplasia as well as cervical cancer is well established, studies on the relationship between HPV infection and risk of preterm birth (PTB) have yielded inconclusive and inconsistent results. Therefore, we conducted a meta-analysis to investigate the association between HPV infection and PTB. The electronic database was searched until July 1, 2014.

Notch-1 and Ki-67 receptor as predictors for the recurrence and prognosis of Kimura's disease.

Kimura's disease (KD) is a rare chronic disease with unknown origin. It remains controversial in KD's diagnosis, treatment, transformation and need further research. The aim of this study is to investigate the clinicopathologic features of KD and the relationship between the expression of Notch-1, Ki-67 receptor and the recurrence of KD.

Direct electrochemistry of glucose oxidase and glucose biosensing on a hydroxyl fullerenes modified glassy carbon electrode.

Direct electrochemistry of glucose oxidase (GOD) was achieved when GOD-hydroxyl fullerenes (HFs) nano-complex was immobilized on a glassy carbon (GC) electrode and protected with a chitosan (Chit) membrane. The ultraviolet-visible absorption spectrometry (UV-vis), transmission electron microscopy (TEM), and circular dichroism spectropolarimeter (CD) methods were utilized for additional characterization of the GOD, GOD-HFs and Chit/GOD-HFs. Chit/HFs may preserve the secondary structure and catalytic properties of GOD.

Direct electron transfer of horseradish peroxidase on a functional nanocomplex modified glassy carbon electrode.

Direct electron transfer of horseradish peroxidase (HRP) was achieved by immobilizing HRP on a functional nanocomplex modified glassy carbon (GC) electrode. The cyclic voltammograms (CVs) of the modified electrode have a pair of well-defined redox peaks with a formal potential (E°') of -26 ± 2 mV versus Ag/AgCl, in 0.05 M, pH7.

A highly efficient nano-cluster artificial peroxidase and its direct electrochemistry on a nano complex modified glassy carbon electrode.

A nano-cluster with highly efficient peroxide activity was constructed based on nafion (NF) and cytochrome c (Cyt c). UV-Vis spectrometry and transmission electron microscopy (TEM) methods were utilized for characterization of the nano-structured enzyme or artificial peroxidase (AP). The nano-cluster was composed of a Chain-Ball structure, with an average ball size of about 40 nm.

[Prokaryotic expression and identification of HPV16 E7 protein].

HPV16 E7 fusion protein was expressed in E. coli BL21, and its applied value for HPV was evaluated. HPV16 E7 gene was amplified by PCR, and cloned into prokaryotic expression vector pGEX6p-1.

[Rapid detection of chromosome number aberration in spontaneously aborted fetuses using fluorescence in situ hybridization].

Objective: To assess the value of fluorescence in situ hybridization (FISH) in the diagnosis of common chromosome number aberration in spontaneously aborted fetuses.

Method: A total of 100 spontaneously aborted fetuses were analyzed by G-banding and by FISH to test chromosome number aberration mainly for chromosomes 13, 18, 21, X and Y, and the results of FISH test was assessed according to those by G-banding test.

Results: FISH results were well consistent with those by G-banding test.

[Evaluations of pre-thromboembolic state in patients undergoing total abdominal or laparoscopic hysterectomy].

Objective: To investigate the influences of total abdominal and laparoscopically hysterectomy on coagulation and fibrinolytic functions.

Methods: Blood samples were taken from 20 patients without high-risk factor of thrombosis before and after total abdominal and laparoscopically hysterectomy. The values of PT, APTT, FG, D-Dimer and AT-III were measured.

[Effect of nitrogen removal simulated by RIP_ N model to a riparian zone in Guangting Reservoir catchment].

An eco-hydrological model system (RIP_ N) was constructed to simulate the nitrogen (N) removal by riparian zone in reservoir catchment scale. As a case study, the N removal in Guanting Reservoir riparian zone from March to September in 2007 was estimated. At same time, the field simulated experiment was carried out in Yanqing experimental station, which lies in the northeast part of the catchment.

[Differential expression of adrenomedullin in the placentas of women with normal and preeclamptic pregnancies in late term].

Objective: To investigate the differential expression of adrenomedullin (ADM) in the placentas of women with normal and preeclamptic pregnancies, and explore the importance of ADM and its signal pathway in the development of preeclampsia.

Methods: Ten pregnant women complicated with preeclampsia during the late term(>or=35 wk) were selected for this study along with 7 normal control pregnant women (>or=39 wk). The total RNA was extracted and sections of fresh placental tissues were prepared, and ADM expressions at mRNA and protein levels in women with normal and preeclamptic pregnancies were determined by Northern blotting and immunohistochemistry, respectively.

[Effects of ambulatory labor analgesia on fetal oxygen saturation].

Objective: To observe the effect of ambulatory labor analgesia on fetal oxygen saturation during labor.

Methods: Sixty parturients with uncomplicated term pregnancies were equally divided, according to their personal preference, into ambulatory analgesia group receiving combined spinal-epidural labor analgesia and control group without analgesia administration. All the parturients with ambulatory labor analgesia received sufentanil and ropivacaine administration.

[Effect of heparin on fetal growth restriction].

Objective: To investigate the clinical effect and safety of heparin in treating fetal growth restriction (FGR).

Methods: A total of 107 women diagnosed with FGR were randomized into three groups. Group 1 (standard heparin group, n = 37) received 50 - 75 mg standard heparin added to 500 ml 5% glucose sodium chloride, infused intravenously in 6 - 8 hours.