Uniform volumetric single-cell processing for organ-scale molecular phenotyping.

Extending single-cell analysis to intact tissues while maintaining organ-scale spatial information poses a major challenge due to unequal chemical processing of densely packed cells. Here we introduce Continuous Redispersion of Volumetric Equilibrium (CuRVE) in nanoporous matrices, a framework to address this challenge. CuRVE ensures uniform processing of all cells in organ-scale tissues by perpetually maintaining dynamic equilibrium of the tissue's gradually shifting chemical environment.

NTRC mediates the coupling of chloroplast redox rhythm with nuclear circadian clock in plant cells.

The intricate interplay between cellular circadian rhythms, primarily manifested in the chloroplast redox oscillations-characterized by diel hyperoxidation/reduction cycles of 2-Cys Peroxiredoxins-and the nuclear transcription/translation feedback loop (TTFL) machinery within plant cells, demonstrates a remarkable temporal coherence. However, the molecular mechanisms underlying the integration of these circadian rhythms remain elusive. Here, we elucidate that the chloroplast redox protein, NADPH-dependent thioredoxin reductase type-C (NTRC), modulates the integration of the chloroplast redox rhythms and nuclear circadian clocks by regulating intracellular levels of reactive oxygen species and sucrose.

Unprecedented short-circuit current density and efficiency of vacuum-deposited organic solar cells based on 8H-thieno[2',3':4,5]thieno[3,2-b] thieno[2,3-d]pyrrole.

Despite the many advantages for industrial mass production, vacuum-deposited organic solar cells (OSCs) suffer from low efficiency, primarily due to the limited molecular library of small-molecule donor and acceptor materials, which remains a significant challenge. Herein, two donor-acceptor-acceptor (D-A-A)-configured small-molecule donors, named TTBTDC and TTBTDC-F were synthesized, using 8H-thieno[2',3':4,5]thieno[3,2-b]thieno[2,3-d]pyrrole (TTP) as a new fused-ring donor unit. Benefiting from the strong electron-donating ability of the TTP moiety and the adoption of the D-A-A molecular configuration, these molecules exhibited strong visible and near-infrared absorption as well as deep-lying highest occupied molecular orbital (HOMO) energy levels.

Four new species of Simon, 1884 (Araneae, Linyphiidae) from Jiangjin District of Chongqing, China.

Four new species of the genus Simon, 1884 are described from Jiangjin, Chongqing: (♂), (♂♀), (♂♀), and (♂♀). The new species exhibit distinctive genital features, such as a bifurcated embolus tip in the male palp of and , and embolus tip unbifurcated in The epigynes display more unique characteristics, such as epigynal plate with a pit ventrally on its frontal face in and , which is absent in all other known species. Detailed descriptions, along with photographs of genital characters, somatic features, and a distribution map, are provided.

Quality Differences in Frozen Mackerel According to Thawing Method: Potential Classification via Hyperspectral Imaging.

Seafood quality preservation remains a critical focus in the food industry, particularly as the freeze-thaw process significantly impacts the freshness and safety of aquatic products. This study investigated quality changes in frozen mackerel subjected to two thawing methods, room temperature (RT) and running water (WT), and assessed the potential of hyperspectral imaging (HSI) for classifying these methods. After thawing, mackerel samples were stored at 5 °C for 21 days, with physicochemical, textural, and spectroscopic analyses tracking quality changes and supporting the development of a spectroscopic classification model.

Synthesis of nickel-boron/reduced graphene oxide for efficient and stable lithium-ion storage.

Electrode material capacities and cycle performances must improve for large-scale applications such as energy storage systems. Numerous investigations have developed cathode materials to improve lithium-ion batteries (LIBs) performance: however, few have examined new anode materials. In this study, we synthesized a Ni-B/reduced graphene oxide (RGO) composites via a simple chemical reaction method to enhance the stability of electrodes in LIBs.

Syphilis cases among pregnant women and newborns in the Military Health System, 2012-2022.

This report presents the rates of maternal syphilis among pregnant women and congenital syphilis among newborns in the Military Health System (MHS) beneficiary population from 2012 to 2022. Medical encounter data from military hospitals and clinics as well as civilian health care facilities were obtained from the Defense Medical Surveillance System (DMSS) to determine pregnancies, live births, and confirmed diagnoses of maternal and congenital syphilis. The rate of maternal syphilis in female MHS beneficiaries increased by 233% between 2012 (n=123, 66.

The Impact of Laser Irradiation on Thin ZrN Films Deposited by Pulsed DC Magnetron Sputtering.

Transition metal nitrides have extensive applications, including magnetic storage devices, hardware resistance coatings, and low-temperature fuel cells. This study investigated the structural, electrical, and mechanical properties of thin zirconium nitride (ZrN) films by examining the effects of laser irradiation times. Thin ZrN films were deposited on glass substrates using pulsed DC magnetron sputtering and irradiated with a diode laser for 6 and 10 min.

Non-destructive assessment of chilling injury in red pepper powder using short-wave-infrared and XGBoost algorithm.

This study aimed to evaluate red pepper powder quality by the extent of chilling injury and develop a method for detecting chilling injury-affected pepper powder. Pepper powder produced from chilling injury-affected pepper fruits exhibited increased bitter amino acids, microbial counts, and biogenic amines and decreased sweetness index and organic acid levels. These quality deteriorations indicate the need to detect chilling injury in pepper powders.

The testis-specific gene 1700030J22Rikis essential for sperm flagellar function and male fertility in mice.

Spermiogenesis is an indispensable process occurring during the later stages of spermatogenesis. Despite multiple proteins being associated with spermiogenesis, the molecular mechanisms that control spermiogenesis remain poorly characterized. In this study, we show that 1700030J22Rik is exclusively expressed in the testis of mice and investigate its roles in spermiogenesis using genetic and proteomic approaches.

A streamlined algorithm for two-dimensional bandgaps and defect-state energy variations in InGaN-based micro-LEDs.

Bandgaps and defect-state energies are key electrical characteristics of semiconductor materials and devices, thereby necessitating nanoscale analysis with a heightened detection threshold. An example of such a device is an InGaN-based light-emitting diode (LED), which is used to create fine pixels in augmented-reality micro-LED glasses. This process requires an in-depth understanding of the spatial variations of the bandgap and its defect states in the implanted area, especially for small-sized pixelation requiring electroluminescence.

Corrosion Resistance of CrO, ZrO, and MnO Oxide Films to Lead-Bismuth Eutectic: A Comparative Study.

In this study, the stability of CrO, ZrO, and MnO oxide films in high-temperature liquid lead-bismuth eutectic (LBE) was systematically investigated using both experimental and first principles calculation methods. The research findings indicated that CrO demonstrated superior structural integrity at corrosion temperatures of both 600 °C and 700 °C and displayed exceptional resistance to LBE corrosion. ZrO demonstrates resistance to LBE infiltration.

Clinical Features of Patients Treated With Hair Transplants in Female Pattern Hair Loss.

Background: Hair transplantation (HT) has been reported to be effective for the treatment of female-pattern hair loss (FPHL). Few studies have investigated HT in FPHL.

Objective: To evaluate the clinical features of FPHL treated with HT and analyze the real-world results of HT.

FAD and NADPH binding sites of YUCCA6 are essential for chaperone activity and oxidative stress tolerance in Arabidopsis thaliana.

Phytohormone auxin plays a pivotal role in governing plant growth, development, and responses to abiotic stresses. YUCCA6 (YUC6), an auxin biosynthetic enzyme belonging to the flavin monooxygenase (FMO) subfamily, converts indole-3-pyruvic acid to indole-3-acetic acid. Our prior investigation uncovered that YUC6 also functions as a thiol-reductase and chaperone in a Cys85-dependent manner, resulting in conferred tolerance to nickel heavy metal stress and drought and delayed leaf senescence.

Development of Saccharomyces cerevisiae accumulating excessive amount of glycogen and its effects on gut microbiota in a mouse model.

Saccharomyces cerevisiae accumulates glycogen, a hyperbranched glucose polymer with multiple bio-functionalities. In this study, mutants of S. cerevisiae that accumulate excessive amounts of glycogen were developed through UV mutagenesis.

pH and Redox Dual-Responsive Nanoparticle with Enhanced Dendritic Cell Maturation for Cancer Therapy.

Type I interferons (IFNs) are essential for activating dendritic cells (DCs) and presenting tumor-associated antigens to T cells. IFNs are primarily produced from DCs among immune cells. A combination of chemotherapy and metalloimmunotherapy induces IFN production by activating the cyclic GMP-AMP synthase-stimulator of interferon genes (cGAS-STING) pathway.

"I am open to self-belief": A study examining the impact of open-label effects on postexercise affect and postintervention exercise behaviour.

The contribution of placebo effects can be measured by manipulating psychological factors, such as positive expectations for exercise outcomes, without the need for traditional placebos (such as inert substances). While placebo effects are well known, research has revealed intriguing findings suggesting that placebos can have beneficial effects even when studies are performed without deception. Open-label placebo (OLP) interventions (prescribed honestly), which harness the power of belief, may represent another effective method for enhancing the benefits of exercise effects.

Wearable Haptics for Orthotropic Actuation Based on Perpendicularly Nested Auxetic SMA Knotting.

Smart wearable tactile systems, designed to deliver different types of touch feedback on human skin, can significantly improve engagement through diverse actuation patterns in virtual or augmented reality environments. Here, a perpendicularly nested auxetic wearable haptic interface is reported for orthotropically decoupled multimodal actuation (WHOA), capable of producing diverse tactile feedback modes with 3D sensory perception. WHOA incorporates shape memory alloy wires that are intricately knotted into an auxetic structure oriented along orthotropic dual axes.

Prediction and Visualization of Total Volatile Basic Nitrogen in Yellow Croaker () Using Shortwave Infrared Hyperspectral Imaging.

In the present investigation, we have devised a hyperspectral imaging (HSI) apparatus to assess the chemical characteristics and freshness of the yellow croaker () throughout its storage period. This system operates within the shortwave infrared spectrum, specifically ranging from 900 to 1700 nm. A variety of spectral pre-processing techniques, including standard normal variate (SNV), multiple scatter correction, and Savitzky-Golay (SG) derivatives, were employed to augment the predictive accuracy of total volatile basic nitrogen (TVB-N)-which serves as a critical freshness parameter.

Phase-Field Simulation of Spinodal Decomposition in U-50Zr Metallic Nuclear Fuel.

During the γ phase-δ phase transition, U-50Zr fuel experiences spinodal decomposition, which has a significant effect on fuel properties. However, little is known about the spinodal decomposition of U-50Zr. The spinodal decomposition behavior in U-50Zr is studied in this research using the phase-field approach.