Molecular engineering of PETase for efficient PET biodegradation.

The widespread utilization of polyethylene terephthalate (PET) has caused a variety of environmental and health problems. Compared with traditional thermomechanical or chemical PET cycling, the biodegradation of PET may offer a more feasible solution. Though the PETase from Ideonalla sakaiensis (IsPETase) displays interesting PET degrading performance under mild conditions; the relatively low thermal stability of IsPETase limits its practical application.

Current advances in the structural biology and molecular engineering of PETase.

Poly(ethylene terephthalate) (PET) is a highly useful synthetic polyester plastic that is widely used in daily life. However, the increase in postconsumer PET as plastic waste that is recalcitrant to biodegradation in landfills and the natural environment has raised worldwide concern. Currently, traditional PET recycling processes with thermomechanical or chemical methods also result in the deterioration of the mechanical properties of PET.

Combining Phage Display Technology with -Designed Epitope Vaccine to Elicit Robust Antibody Responses against Emerging Pathogen Tilapia Lake Virus.

Antigen epitope identification is a critical step in the vaccine development process and is a momentous cornerstone for the development of safe and efficient epitope vaccines. In particular, vaccine design is difficult when the function of the protein encoded by the pathogen is unknown. The genome of Tilapia lake virus (TiLV), an emerging virus from fish, encodes protein functions that have not been elucidated, resulting in a lag and uncertainty in vaccine development.

Biomimetic nanovaccine based on erythrocyte membrane enhances immune response and protection against tilapia lake virus.

An infectious disease emerged in recent years, Tilapia Lake Virus Disease (TiLVD), has severely restricted the development of global tilapia industry. Vaccination has proved potential strategy to prevent its causative agent Tilapia Lake Virus (TiLV) infectious. However, the response intensity of subunit vaccine is limited by its low immunogenicity, thus inclusion of adjuvants is required.

Crystal phase transition and polyhedron transformation towards the evolution of photoluminescence and the improvement of thermal stability in efficient blue-emitting BaSrAlSiO:Eu.

Investigations into novel single-phase phosphors with outstanding luminescence properties and excellent thermal stability are urgently needed in the lighting field. In this work, a crystal phase transition and polyhedron transformation strategy cation substitution has been proposed. controlling the Sr/Ba ratio, the structural evolution of the phosphor from a monocelsian phase to a hexacelsian or feldspar phase and the variation of the local environments of Eu sites are correspondingly studied in BaSrAlSiO:0.

Optimization of immunization procedure for SWCNTs-based subunit vaccine with mannose modification against spring viraemia of carp virus in common carp.

Immersion vaccination of single-walled carbon nanotubes loaded with mannose-modified glycoprotein (SWCNTs-MG) vaccine has been proved to be effective in preventing spring viraemia of carp virus (SVCV). Immunization procedure has immense consequence on the immune effect of the immersion vaccine. However, immunization procedure optimization for SWCNTs-MG vaccine against SVCV has not been reported.

Highly Sensitive Dual-Mode Optical Thermometry in Double-Perovskite Oxides via Pr/Dy Energy Transfer.

As increasing demand for noncontact temperature sensing, the development of a high-performance optical thermometer probe is more and more urgent. In this work, an efficient dual-mode optical thermometry strategy based on the Pr/Dy energy transfer (ET) in some typical double-perovskite oxides is presented, which offers a promising way to design FIR/lifetime dual-mode optical thermometry with excellent temperature-measuring sensitivity and signal discrimination. According to this strategy, double-perovskite LaMgTiO:Pr/Dy phosphors are successfully synthesized.

Preliminary screening and immunogenicity analysis of antigenic epitopes of spring viremia of carp virus.

Glycoprotein (G) is the most common gene used in SVCV vaccine constructions. To identify the major immunogenicity determinant region of SVCV G gene, herein we truncated G gene to 4 parts (G-1, G-2, G-3 and G-4). Bioinformatics and the enzyme linked immunosorbent assay (ELISA) were used to identify the antigenicity of these 4 truncated G proteins.

Evaluation of immune response and protection against spring viremia of carp virus induced by a single-walled carbon nanotubes-based immersion DNA vaccine.

Spring viremia of carp virus (SVCV) has caused mass mortality in cyprinids, with case fatality rates of young fish up to 90%, resulting in enormous economic losses in the aquaculture industry. Immersion vaccination is considered as the most effective method for juvenile fish to combating disease, due to its convenience for mass vaccination and stress-free administration. However, immune responses following immersion vaccination are generally less robust and of shorter duration as those induced through intraperitoneal injection.