Clinical and chest CT manifestations of Talaromycosis marneffei: a retrospective study comparing anti-IFN-γ autoantibodies-positive and HIV-positive cases.

Background: To explore the clinical and chest CT features of Talaromycosis marneffei (TSM), and to compare the differences between anti‑IFN‑γ autoantibodies‑positive and HIV-positive cases.

Methods: Clinical data and chest CT images of 54 HIV-negative patients with Talaromyces marneffei (TM) infection and positive anti-interferon-γ (anti-IFN-γ) autoantibody were retrospectively analyzed. Ninety-three HIV-positive patients with TM infection during the same period were included as controls.

Magnetic resonance imaging-based rim enhancement could effectually predict poor prognosis in hepatocellular carcinoma: a meta-analysis.

Recent studies have initially shown that MRI-based rim enhancement associates with poor prognosis in hepatocellular carcinoma (HCC) patients, but their sample sizes are small, leading to a necessary of comprehensive analyses to make a relatively solid statement. Thus, this meta-analysis aimed to summarize the correlation between MRI-based rim enhancement and prognosis in HCC patients. Until March 2023, a literature search was conducted on Web of Science, PubMed, EMBASE, Cochrane, CNKI, Wangfang, and CQVIP databases in order to identify studies that report the correlation between MRI-based rim enhancement and the prognosis of HCC patients.

Topologic Reorganization of White Matter Connectivity Networks in Early-Blind Adolescents.

Blindness studies are important models for the comprehension of human brain development and reorganization, after visual deprivation early in life. To investigate the global and local topologic alterations and to identify specific reorganized neural patterns in early-blind adolescents (EBAs), we applied diffusion tensor tractography and graph theory to establish and analyze the white matter connectivity networks in 21 EBAs and 22 age- and sex-matched normal-sighted controls (NSCs). The network profiles were compared between the groups using a linear regression model, and the associations between clinical variables and network profiles were analyzed.

Identification of a Novel Signature Predicting Overall Survival in Head and Neck Squamous Cell Carcinoma.

Head and neck squamous cell carcinoma (HNSCC) is a highly heterogeneous tumor with a high incidence and poor prognosis. Therefore, effective predictive models are needed to evaluate patient outcomes and optimize treatment. Robust Rank Aggregation (RRA) method was used to identify highly robust differentially-expressed genes (DEGs) between HNSCC and normal tissue in 9 GEO and TCGA datasets.

Construction of a Promising Tumor-Infiltrating CD8+ T Cells Gene Signature to Improve Prediction of the Prognosis and Immune Response of Uveal Melanoma.

Background: CD8+ T cells work as a key effector of adaptive immunity and are closely associated with immune response for killing tumor cells. It is crucial to understand the role of tumor-infiltrating CD8+ T cells in uveal melanoma (UM) to predict the prognosis and response to immunotherapy.

Materials And Methods: Single-cell transcriptomes of UM with immune-related genes were combined to screen the CD8+ T-cell-associated immune-related genes (CDIRGs) for subsequent analysis.

Diffusion-weighted imaging with background body signal suppression (DWIBS) distinguishes benign lesions from malignant pulmonary solitary lesions.

This study aimed to determine applicable value of DWIBS in diagnosis of solitary pulmonary lesions. This study involved 32 solitary lung disease patients. T1W1, T2W1, T2WI-SPAIR were examined using MRI scanner and analyzed with View-forum 6.

CT features of COVID-19 patients with two consecutive negative RT-PCR tests after treatment.

The objective of this study is to expound the CT features of COVID-19 patients whose throat swab samples were negative for two consecutive nucleic acid tests after treatment. We retrospectively reviewed 46 COVID-19 patients with two consecutive negative RT-PCR tests after treatment. The cases were divided into moderate group and severe/critical group according to disease severity.

Differential gene expression associated with inflammation in peripheral blood cells of patients with pneumoconiosis.

Objectives: To study expression changes in inflammation-related genes in peripheral blood of patients with pneumoconiosis and to explore the possibility of these genes as pneumoconiosis biomarkers.

Methods: Peripheral blood samples of patients with pneumoconiosis patients and controls were collected, and total RNA of the blood cells were extracted and reverse transcribed to cDNA. Screenings of deferentially expressed genes associated with inflammation between patients with pneumoconiosis and controls were performed using real-time quantitative PCR array and the expressions of the three most upregulated genes were confirmed by real-time PCR.

Cytogenetic analysis of peripheral blood lymphocytes, many years after exposure of workers to low-dose ionizing radiation.

The present study aims to measure chromosomal aberrations and micronuclei in peripheral blood lymphocytes from 25 subjects exposed to 0.10-0.33 Gy external or internal irradiation 32-41 years ago using conventional cytogenetic analysis methods.

Assessment of retrospective dose estimation, with fluorescence in situ hybridization (FISH), of six victims previously exposed to accidental ionizing radiation.

The present study aims to evaluate the use of the fluorescence in situ hybridization (FISH) translocation assay for retrospective dose estimation of acute accidental exposure to radiation in the past. Reciprocal translocation analysis by FISH with three whole-chromosome probes was performed on normal peripheral blood samples. Samples were irradiated with 0-5Gy (60)Co γ-rays in vitro, and dose-effect curves were established.

[Serum levels of CC-Chemokine ligand 18 in the patients with pneumoconiosis].

Objective: To measure the peripheral serum levels of CC-chemokine ligand-18 (CCL-18) in patients with pneumoconiosis, and to investigate the feasibility of the index asa potential biomarker for pneumoconiosis.

Methods: Seventy-seven male patients with pneumoconiosis (stage 1:40 cases, stage 2:22 cases, stage 3:15 cases), including 42 cases of silicosis and 35 cases of coal worker pneumoconiosis, were enrolled as subjects, and 162 healthy male physical examinees in our hospital were used as controls. A fasting blood sample (3 ml) was collected from the peripheral venous blood of each patient or control.

[The endoscopic and clinical features of Indigo Naturalis-associated ischemic lesions of colonic mucosa].

Objective: By analysing the clinical features of Indigo Naturalis-associated ischemic lesion of colon mucosa to improve the precautionary and therapeutic level of the disease.

Methods: Thirteen patients diagnosed as Indigo Naturalis-associated ischemic lesion of colon mucosa in Peking University Third Hospital from 2005 to 2010 were reviewed. The endoscopic and clinical features were analysed.

A structurally novel salt-regulated promoter of duplicated carbonic anhydrase gene 1 from Dunaliella salina.

It has been demonstrated that the duplicated carbonic anhydrase is induced by salt in the Dunaliella salina (D. salina) and duplicated carbonic anhydrase 1 (DCA1) is a member of carbonic anhydrase family. The purpose of this study was to identify whether both the DCA1 gene and its promoter from D.

The nitrate reductase gene-switch: a system for regulated expression in transformed cells of Dunaliella salina.

The control of promoter activity by nitrogen source has recently emerged as an intriguing system for regulated expression of the heterologous genes. The purpose of this work was to investigate whether heterologous gene expression in transgenic Dunaliella salina would be controlled by an inducible promoter. Here we identify that the nitrate reductase (NR) transcripts of D.

[Biological dose assessment by the analyses of chromosomal aberrations and CB micronuclei in two victims accidentally exposed to 60Co gamma-rays].

Biological doses were estimated by using the yields of dicentrics plus rings(dic+r) and cytokinesis-block micronuclei (CBMN) for two victims of the accident radiation occurred on Mar 6,2000 in the City of Xuchang(victim A), and Jun 26,2000 in the City of Kaifeng(victim B), Henan Province,respectively. The results indicated that the equvalent whole body doses based on the standard dose-response curves established by the analyses of dic+r and CBMN were estimated to be 1.44Gy and 1.

The actin gene promoter-driven bar as a dominant selectable marker for nuclear transformation of Dunaliella salina.

A 5'-flanking region of an actin gene from the green unicellular alga Dunaliella salina (D. salina) was cloned using a genome-walking method by PCR and its structural features were characterized. Two repetitive sequences found, over 75 bp in length each, were located at position -573 and -424 bp,respectively, relative to the AUG codon.

[Expression of calprotectin in colon mucosa and fecal of patients with ulcerative colitis].

Objective: To investigate the correlation between the disease activity of ulcerative colitis (UC) and the immunohistochemical distribution of calprotectin in colon mucosa,as well as the levels of calprotectin in fecal.

Methods: Monoclonal antibody against calprotectin was used to investigate the distribution of these proteins in colon mucosa from 42 patients with ulcerative colitis and 20 healthy controls. ELISA assay was used to measure the concentrations of calprotectin in fecal.

Characteristics and therapeutic efficacy of sulfasalazine in patients with mildly and moderately active ulcerative colitis.

Aim: To investigate the characteristics and short-term efficacy of sulfasalazine (SASP) in patients with mildly and moderately active ulcerative colitis (UC).

Methods: Two hundred and twenty-eight patients with mildly and moderately active UC were recruited, 106 patients in 1993-1995, and 122 patients in 2000-2002, they were assigned as the 1990s group (n = 106) and the 2000s group (n = 122), prospectively. The general characteristics, clinical manifestations, colonoscopic and histological data were compared between the two groups.

[Cloning and characterization of Hsp70a cDNA fragment of Dunaliella salina].

The present study is to obtain heat shock protein 70a cDNA fragment from Dunaliella salina. Two pairs of degenerate primers were designed according to conserved motifs of DIDLGTT,DQGNRTTP,PAYFNDS and ATKDAG of the homologous amino acid sequences and used to amplify hsp70a cDNA fragment from heat-shock-treated Dunaliella salina by nest PCR technique. The resulting PCR products were inserted into T-vector then transformed into JM109.