Rational design of carbon dot nanozymes for ratiometric dual-signal and smartphone-assisted visual detection of nitrite in food matrices.

Developing reliable nitrite (NO) sensors is essential for food safety and reducing health risks from NO exposure. In this study, we strategically designed nitrogen-doped carbon dot (N-CD) nanozymes to establish an accessible dual-signal ratiometric sensing system for detecting NO in food matrices. This system utilizes the photoluminescence and enzyme-like properties of N-CD nanozymes combined with NO-triggered diazotization reactions of substrates such as o-phenylenediamine (OPD) or 3,3',5,5'-tetramethylbenzidine (TMB).

Drug-Primed Self-Assembly of Platinum-Single-Atom Nanozyme to Regulate Cellular Redox Homeostasis Against Cancer.

Single-atom nanozymes (SAzymes) with high catalytic activity exhibit the potential to disequilibrate the reactive oxygen metabolic balance in the tumor microenvironment (TME), which contains several endogenous reductive substances such as glutathione (GSH). Herein, a novel nano-assembly (CDs@Pt SAs/NCs@DOX) is first constructed using drug-primed platinum (Pt) single-atom or nanocluster nanozymes with a Pt loading of 34.8%, which exhibits prominent dual enzymatic activities to mimic peroxidase (POD) and glutathione oxidase (GSHOx).

Robust solvatochromic carbon quantum dots for selective detection of water and Sn and specific lipid imaging.

Developing carbon quantum dots (CQDs) with the solvatochromic effect and exploring multifunctional applications remains challenging. Herein, robust solvatochromic carbon quantum dots (RS-CQDs) with emission shift up to ∼62 nm from yellow to red was fabricated by the hydrothermal method. The RS-CQDs was used to detect water and Sn in the linear ranges and limits of detection of 2.

Intelligently design primary aromatic amines derived carbon dots for optical dual-mode and smartphone imaging detection of nitrite based on specific diazo coupling.

Primary aromatic amines derived carbon dots (PAA-CDs) with the protonated amino groups and high quantum yield of 46% were favorably obtained by one-step solvothermal treatment of m-phenylenediamine (m-PDA) in acidic environment. The interaction between the PAA-CDs and nitrite (NO) was inherited the characteristic reaction of m-PDA (a primary aromatic amine) and NO, resulting in strong fluorescence quenching and obvious absorption variation of the PAA-CDs. Meanwhile, a chromogenic reaction of diazo coupling can cause significant color changes.

A fluorometric and colorimetric dual-readout nanoprobe based on Cl and N co-doped carbon quantum dots with large stokes shift for sequential detection of morin and zinc ion.

A novel visual nanoprobe was developed for the sequential detection of morin and zinc ion (Zn) based on Cl and N co-doped carbon quantum dots (ClNCQDs) via a fluorometric and colorimetric dual-readout mode. The yellow fluorescence ClNCQDs was synthesized by the one-step hydrothermal treatment of o-chlorobenzoic acid and p-phenylenediamine. The most distinctive property of the ClNCQDs is the large stokes shift (177 nm), which is significantly higher than other reported CQDs.

Ratiometric fluorescent sensors for sequential on-off-on determination of riboflavin, Ag and l-cysteine based on NPCl-doped carbon quantum dots.

The fluorescent sensor, especially ratiometric fluorescent sensor, is one of the most important applications for CQDs, which is becoming a research hotspot. Herein, carbon quantum dots co-doped with nitrogen, phosphorus and chlorine (NPCl-CQDs) were synthesized by acid-base neutralization reaction exothermic carbonization method. The as-fabricated NPCl-CQDs could emit blue fluorescence and possess excellent fluorescence properties.

Nitrogen, sulfur, phosphorus, and chlorine co-doped carbon nanodots as an "off-on" fluorescent probe for sequential detection of curcumin and europium ion and luxuriant applications.

Nitrogen, sulfur, phosphorus, and chlorine co-doped carbon nanodots (NSPCl-CNDs) were fabricated by acid-base neutralization and exothermic carbonization of glucose. The obtained NSPCl-CNDs possess excellent fluorescence properties and good biocompatibility. Curcumin (Cur) can dramatically quench the fluorescence of NSPCl-CNDs based on a synergistic effect of electrostatic interaction, inner filter effect, and static quenching, so a "turn-off" fluorescent probe for Cur detection was constructed with linear ranges of 0.

A Non-Synonymous Single Nucleotide Polymorphism in the HJURP Gene Associated with Susceptibility to Hepatocellular Carcinoma among Chinese.

Objective: HJURP (Holliday Junction-Recognizing Protein) plays dual roles in DNA repair and in accurate chromosome segregation during mitosis. We examined whether the single nucleotide polymorphisms (SNPs) of HJURP were associated with the risk of occurrence of hepatocellular carcinoma (HCC) among chronic hepatitis B virus (HBV) carriers from well-known high-risk regions for HCC in China.

Methods: Twenty-four haplotype-tagging SNPs across HJURP were selected from HapMap data using the Haploview software.

Mitochondrial calcium uniporter protein MCU is involved in oxidative stress-induced cell death.

Mitochondrial calcium uniporter (MCU) is a conserved Ca(2+) transporter at mitochondrial in eukaryotic cells. However, the role of MCU protein in oxidative stress-induced cell death remains unclear. Here, we showed that ectopically expressed MCU is mitochondrial localized in both HeLa and primary cerebellar granule neurons (CGNs).

Generation of Calhm1 knockout mouse and characterization of calhm1 gene expression.

Alzheimer's disease (AD) is the most common neurodegenerative disease among elderly people worldwide. Several genes have been validated to be associated with AD, and calcium homeostasis modulator 1 (Calhm1) is the latest suspected one. To investigate the biological and pathological function of Calhm1 systematically, we generated a Calhm1 conventional knockout mouse.

Lysine methylation of FOXO3 regulates oxidative stress-induced neuronal cell death.

FOXO transcription factors have a critical role in oxidative stress-induced neuronal cell death. A variety of post-translational modifications of FOXO family proteins have been reported, including phosphorylation, acetylation, ubiqutination and recently arginine methylation. Here, we demonstrate that the methyltransferase Set9 methylates FOXO3 at lysine 270.

Ca(2+) release induced by cADP-ribose is mediated by FKBP12.6 proteins in mouse bladder smooth muscle.

We examined the role and molecular mechanism of cADPR action on Ca(2+) spark properties in mouse bladder smooth muscle. Dialysis of cADPR with patch pipettes increased frequency and amplitude of spontaneous transient out currents (STOCs) to 6.1+/-0.