Publications by authors named "Yumiko Tohma-Aiba"
Production of native recombinant human midkine in the yeast, Pichia pastoris.
Protein Expr Purif
February 2003
Article Synopsis
- Recombinant human midkine (rh-midkine) was produced successfully in the yeast Pichia pastoris using a specific secretion signal and promoter, yielding about 360 mg in 1L of medium.
- The purified rh-midkine was confirmed to be identical to natural human midkine through mass spectrometry, which showed a single molecular ion signal at 13241.2 m/z.
- Additionally, rh-midkine was functionally validated by demonstrating its activity in a cell-proliferation assay, confirming its biological relevance.
Characterization of partially truncated human midkine expressed in Pichia pastoris.
Biosci Biotechnol Biochem
June 2002
Recombinant human midkine (rh-midkine) was expressed under the control of the AOX1 gene promoter in Pichiapastoris. Approximately 640 mg of rh-midkine was secreted into one liter of medium of the high cell-density fermentation. The protein processing of the rh-midkine was done efficiently and correctly in P.
View Article and Find Full Text PDF