Study on methane degradation by microbial agents based on chelating wetting agent carriers.

Due to the low permeability characteristics of the deep gas-containing coal seam, the conventional prevention and control measures that cannot solve the problems of gas outbursts are unsatisfactory for the prevention and control of the coal and gas outbursts disaster. Therefore, in this study, a strain of methane-oxidizing bacteria M with high-pressure resistance, strong resistance, and high methane degradation rate was selected from coal mines. The growth and degradation abilities of M in chelating wetting agent solutions to assess its adaptability and find the optimal agent-to-M07 ratio.

Experimental study on the impact of "IDS + JFCS" complex wetting agent on the characteristics of coal bodies.

As China's coal mines have transitioned to deep mining, the ground stress within the coal seams has progressively increased, resulting in reduced permeability and poor wetting ability of conventional wetting agents. Consequently, these agents have become inadequate in fulfilling the requirements for preventing washouts during deep mining operations. In response to the aforementioned challenges, a solution was proposed to address the issues by formulating a composite wetting agent.

Applications, prospects and challenges of metal borides in lithium sulfur batteries.

Although the lithium-sulfur (Li-S) battery has a theoretical capacity of up to 1675 mA h g, its practical application is limited owing to some problems, such as the shuttle effect of soluble lithium polysulfides (LiPSs) and the growth of Li dendrites. It has been verified that some transition metal compounds exhibit strong polarity, good chemical adsorption and high electrocatalytic activities, which are beneficial for the rapid conversion of intermediate product in order to effectively inhibit the "shuttle effect". Remarkably, being different from other metal compounds, it is a significant characteristic that both metal and boron atoms of transition metal borides (TMBs) can bind to LiPSs, which have shown great potential in recent years.

Improving performances of Lithium-Sulfur cells via regulating of VSe functional mediator with Doping-Defect engineering and Electrode-Separator integration strategy.

The sluggish redox kinetics and the severe shuttle effect of soluble lithium polysulfides (LiPSs) are the main key issues which would hinder the development of lithium-sulfur (Li-S) batteries. In this work, a nickel-doped vanadium selenide in-situ grows on reduced graphene oxide(rGO) to form a two-dimensional (2D) composite Ni-VSe/rGO by a simple solvothermal method. When it is used as a modified separator in Li-S batteries, the Ni-VSe/rGO material with the doped defect and super-thin layered structure can greatly adsorb LiPSs and catalyze the conversion reaction of LiPSs, resulting in effectively reducing LiPSs diffusion and suppressing the shuttle effect.

Active Sulfur-Host Material VS with Surface Defect Engineering: Intercalation-Conversion Hybrid Cathode Boosting Electrochemical Performance of Li-S Batteries.

Transition-metal sulfides as late-model electrocatalysts usually remain inactive in lithium-sulfur (Li-S) batteries in spite of their advantages to accelerate the rapid conversion of lithium polysulfides (LiPSs). Herein, a series of cobalt-doped vanadium tetrasulfide/reduced graphene oxide (%Co-VS/rGO) composites with an ultrathin layered structure as an active sulfur-host material are prepared by a one-pot hydrothermal method. The well-designed two-dimensional ultrathin 3%Co-VS/rGO with heteroatom architecture defects (defect of Co-doping and defect of S-vacancies) can significantly improve the adsorption ability on LiPSs, the electrocatalytic activity in the LiS potentiostatic deposition, and the active sulfur reduction/oxidation conversion reactions and greatly boost the electrochemical performances of Li-S batteries.

Extraordinary electrochemical performance of lithium-sulfur battery with 2D ultrathin BiOBr/rGO sheet as an efficient sulfur host.

There are many challenges such as the shuttling effect of soluble lithium polysulfides species (LiPSs) and the slow solid-state conversion between LiS and LiS in the development process of lithium-sulfur battery (LSB), so it is vital how to design and fabricate sulfur hosts with strong adsorption and good electrocatalysis. In this work, BiOBr in-situ forms onto both sides of reduced graphene oxide (rGO) to obtain a novel ultrathin BiOBr/rGO sheet, then self-constructing a hydrogel cylinder in shape, via a one-step hydrothermal process. The BiOBr/rGO composite with sandwich structure not only shows the outstanding adsorption effect on LiPSs, resulting from a strong bonding interaction between BiOBr/rGO and LiS demonstrated by XPS technique, but also exhibits the extraordinary electrocatalytic performance on both the LiPSs conversion reaction in cyclic voltammetry experiment of symmetric cell and the LiS nucleation process in potentiostatic deposited experiment, which will significantly improve the electrochemical performance of LSB.

Mass spectrometric quantification of the binding ratio of metal-based anticancer complexes with protein thiols.

Rationale: The binding ratio of metal complexes with cysteinyl thiols in proteins plays an important role in deciphering the mechanisms of action of therapeutic metal complexes, but its analysis is still a significant challenge. In this work, a quantitative mass spectrometry method is developed to determine the binding ratio of metal-based anticancer complexes with cysteines in human copper chaperone protein Atox1.

Methods: A novel strategy based on a thiol-specific stable isotopic labelling reagent was developed to determine the binding ratio of metal-based anticancer complexes, namely cisplatin and organometallic ruthenium complex [(η -biphenyl)RuCl(en)]PF (en = ethylenediamine), with the cysteinyl residues of Atox1.

Impaired cocaine-induced behavioral plasticity in the male offspring of cocaine-experienced sires.

Our previous work indicated that male, but not female, offspring of cocaine-experienced sires display blunted cocaine self-administration. We extended this line of investigation to examine behavioral sensitization, a commonly used model of cocaine-induced behavioral and neuronal plasticity. Results indicated that male, but not female, offspring of cocaine-taking sires showed deficits in the ability of repeated systemic cocaine injections to induce augmented locomotor activity.

Interrogation of nonconserved human adipose lincRNAs identifies a regulatory role of in adipocyte metabolism.

Long intergenic noncoding RNAs (lincRNAs) have emerged as important modulators of cellular functions. Most lincRNAs are not conserved among mammals, raising the fundamental question of whether nonconserved adipose-expressed lincRNAs are functional. To address this, we performed deep RNA sequencing of gluteal subcutaneous adipose tissue from 25 healthy humans.

The NANCI-Nkx2.1 gene duplex buffers Nkx2.1 expression to maintain lung development and homeostasis.

A subset of long noncoding RNAs (lncRNAs) is spatially correlated with transcription factors (TFs) across the genome, but how these lncRNA-TF gene duplexes regulate tissue development and homeostasis is unclear. We identified a feedback loop within the NANCI (Nkx2.1-associated noncoding intergenic RNA)-Nkx2.

Preferential Phosphorylation on Old Histones during Early Mitosis in Human Cells.

How histone post-translational modifications (PTMs) are inherited through the cell cycle remains poorly understood. Canonical histones are made in the S phase of the cell cycle. Combining mass spectrometry-based technologies and stable isotope labeling by amino acids in cell culture, we question the distribution of multiple histone PTMs on old versus new histones in synchronized human cells.

Monitoring Cellular Phosphorylation Signaling Pathways into Chromatin and Down to the Gene Level.

Protein phosphorylation, one of the most common and important modifications of acute and reversible regulation of protein function, plays a dominant role in almost all cellular processes. These signaling events regulate cellular responses, including proliferation, differentiation, metabolism, survival, and apoptosis. Several studies have been successfully used to identify phosphorylated proteins and dynamic changes in phosphorylation status after stimulation.

Drawbacks in the use of unconventional hydrophobic anhydrides for histone derivatization in bottom-up proteomics PTM analysis.

MS-based proteomics has become the most utilized tool to characterize histone PTMs. Since histones are highly enriched in lysine and arginine residues, lysine derivatization has been developed to prevent the generation of short peptides (<6 residues) during trypsin digestion. One of the most adopted protocols applies propionic anhydride for derivatization.

Combining genomic and proteomic approaches for epigenetics research.

Epigenetics is the study of changes in gene expression or cellular phenotype that do not change the DNA sequence. In this review, current methods, both genomic and proteomic, associated with epigenetics research are discussed. Among them, chromatin immunoprecipitation (ChIP) followed by sequencing and other ChIP-based techniques are powerful techniques for genome-wide profiling of DNA-binding proteins, histone post-translational modifications or nucleosome positions.

Reactions of an organoruthenium anticancer complex with 2-mercaptobenzanilide--a model for the active-site cysteine of protein tyrosine phosphatase 1B.

The organometallic anticancer complex [(η(6)-p-cymene)Ru(en)Cl]PF(6) (1, en = ethylenediamine) readily reacts with thiols and forms stable sulfenate/sulfinate adducts which may be important for its biological activity. Protein tyrosine phosphatase 1B (PTP1B), a therapeutic target, contains a catalytic cysteinyl thiol and is involved in the regulation of insulin signaling and the balance of protein tyrosine kinase activity. On oxidation, the catalytic Cys215 can form an unusual sulfenyl-amide intermediate which can subsequently be reduced by glutathione.

Elucidation of the binding sites of sodium dodecyl sulfate to β-lactoglobulin using hydrogen/deuterium exchange mass spectrometry combined with docking simulation.

Hydrogen/deuterium exchange mass spectrometry (H/DX MS) has become a powerful tool to investigate protein-protein and protein-ligand interactions, but it is still challenging to localize the interaction regions/sites of ligands with pepsin-resistant proteins such as lipocalins. β-Lactoglobulin (BLG), a member of the lipocalin family, can bind a variety of small hydrophobic molecules including retinols, retinoic acids, and long linear fatty acids. However, whether the binding site of linear molecules locates in the external groove or internal cavity of BLG is controversial.

Folding and aggregation of cationic oligo(aryl-triazole)s in aqueous solution.

Cationic aryl triazole oligomers have been synthesized through "click chemistry". The results show that cationic aryl triazole oligomers adopt a helical conformation in water or in a mixture of water and methanol, but prevail as a random-coiled conformation in methanol. Importantly, circular dichroism spectroscopy and dynamic light scattering experiments revealed that cationic oligomers aggregated intermolecularly to form higher order architectures with a helical sense opposite to that of the individual helix, which eventually led to the formation of aggregates with sizes in the range 100-500 nm.