Ygr125w/Vsb1-dependent accumulation of basic amino acids into vacuoles of Saccharomyces cerevisiae.

The Ygr125w was previously identified as a vacuolar membrane protein by a proteomic analysis. We found that vacuolar levels of basic amino acids drastically decreased in ygr125wΔ cells. Since N- or C-terminally tagged Ygr125w was not functional, an expression plasmid of YGR125w with HA3-tag inserted in its N-terminal hydrophilic region was constructed.

Control of intracellular localization and function of Cx43 by SEMA3F.

Connexin genes are considered to form a family of tumor-suppressor genes. However, the mechanism of connexin-mediated growth control is not well understood. We now provide several lines of evidence which suggest that SEMA3F, a member of the class 3 semaphorin family, which is also reported to be a tumor suppressor, controls the intracellular localization and function of connexin 43 (Cx43).

Role of the cytoplasmic loop domain of Cx43 in its intracellular localization and function: possible interaction with cadherin.

We have previously shown that intracellular trafficking and function of connexin (Cx) 26 and Cx43 are controlled by E-cadherin. In the present study, we attempted to determine which part of Cx43 is involved in this control mechanism. Since Cx26 has a very short C terminus in the cytoplasm, we hypothesized that the C-terminal domain may not be important for this process and, indeed, found that green fluorescence protein (GFP)-tagged Cx43DeltaC (deleted from the codon 239) moved to the plasma membrane both in P3/22(E), a mouse papilloma cell line which expresses E-cadherin, and HeLa cells only at high calcium culture conditions.

[Four cases of pollen-food allergy syndrome suspected the cross reactivity including latex].

Background: Generally it is recognized that the occurrence of fruit allergy is attributed not to the sensitization of itself but to the cross reactivity with pollens or latex. But the relationship as to the sensitization between pollen and latex is obscure. So we aimed to investigate the relation of sensitization among pollens, fruits and latex.

An Efficient Acylation of Tertiary Alcohols with Isopropenyl Acetate Mediated by an Oxime Ester and Cp(2)Sm(thf)(2).

An efficient method for the acylation of tertiary alcohols with isopropenyl acetate (1) by the use of an oxime and Cp(2)Sm(thf)(2) as catalyst was developed. Thus, various types of tertiary alcohols could be acylated with 1 in the presence of a catalytic amount of cyclohexanone oxime acetate (2) and Cp(2)Sm(thf)(2) under mild conditions to form the corresponding acetates in excellent yields. Acid-sensitive terpene alcohols such as linalool were successfully acetylated by the present method to give acetyl linalool in quantitative yield.

Acylation of Alcohols and Amines with Vinyl Acetates Catalyzed by Cp(2)Sm(thf)(2).

Cp(2)Sm(thf)(2) was found to be an efficient catalyst for the acylation of alcohols and amines with esters under mild conditions. In the present acylation, vinyl and isopropenyl acetates served as good acylating agents. Thus, a variety of alcohols and amines underwent acylation with vinyl and isopropenyl acetates in the presence of Cp(2)Sm(thf)(2) to give the corresponding esters and amides in good to excellent yields.