The effects of grazer exclosure duration on soil microbial communities on the Qinghai-Tibetan Plateau.

While determining the response of soil microbes to grazer exclosure duration is critical to understanding ecosystem restoration processes, few studies have focused on this issue. With seasonal grazing as a control, microbes of alpine grassland soils under 5, 13, 22, and 39 years of grazer exclosure situated in the eastern part of the Qinghai-Tibetan Plateau, were examined. Microbial diversity was determined through Illumina high-throughput sequencing of the 16S rRNA gene and an internal transcription spacer (ITS).

[Inhibition of excretory - secretory antigens on growth of murine Lewis lung carcinoma].

Objective: To investigate the effect of excretory-secretory antigens (ESA) on CD4 CD25 Foxp3 T (Treg) cells in mice carrying Lewis lung carcinoma, and examine the inhibitory effect of ESA on tumor growth.

Methods: C57BL/6 mice were randomly assigned into the PBS group ( = 14) and the Lewis group ( = 34). Mice in the Lewis group were subcutaneously injected with 2 × 105 Lewis lung carcinoma cells in the right axilla, while animals in the PBS group were injected with the same volume of sterile PBS.

[Experience and analysis of excellent works of human parasitology in national medical colleges and universities].

Teaching competition is an effective way for college and university teachers to improve their teaching skills. Based on the teaching practice and experience in medical parasitology, this paper discusses several key issues in teaching competition including topics, teaching designs and teaching methods. It provides references for the teachers in department of parasitology of universities and colleges to improve the quality of classroom teaching.

[Anti-tumor effect of infection on melanoma in mice].

Objective: To explore the anti-tumor effect of 17XL strains of (P.y) infection on melanoma in mice.

Methods: B16F10 tumor cells were axillarilly injected into the right flank of 20 C57BL/6 mice to establish tumor-bearing mouse models.

Optimized codon usage enhances the expression and immunogenicity of DNA vaccine encoding Taenia solium oncosphere TSOL18 gene.

Cysticercosis due to larval cysts of Taenia solium, is a serious public health problem affecting humans in numerous regions worldwide. The oncospheral stage-specific TSOL18 antigen is a promising candidate for an anti-cysticercosis vaccine. It has been reported that the immunogenicity of the DNA vaccine may be enhanced through codon optimization of candidate genes.

[Sequence homology analysis on lactate dehydrogenase (LDH) of Plasmodium vivax Anhui isolates].

DNA in dried blood spots of 39 vivax malaria patients (2009-2010) from Anhui (Bengbu urban district and counties of Wuhe, Huaiyuan, Mengcheng and Lixin) was extracted. The Plasmodium vivax LDH (PvLDH) gene was amplified, cloned and sequenced. The sequences were subjected to NCBI Blast program.

[Role of CD8+ T cells in the tumor growth delay induced by Toxoplasma gondii excreted-secreted antigen in B16F10 mouse melanoma model].

Objective: To observe the role of CD8+ T cells in the tumor growth delay induced by Toxoplasma gondii excreted-secreted antigens (TgESA) in B16FI0 mouse melanoma model in the early stage.

Methods: TgESA were prepared by incubating T. gondii tachyzoites for 12 h in vitro.

[Malaria epidemiologic characteristics in Wuhe County of Anhui Province from 2009 to 2011].

Objective: To understand the malaria epidemiologic characteristics in Wuhe County, Anhui Province from 2009 to 2011, so as to provide the evidence for formulating effective malaria control and prevention interventions.

Methods: The data of malaria cases from the reporting system and malaria epidemiological investigations were collected and analyzed statistically in Wuhe County from 2009 to 2011.

Results: The total number of malaria cases was 349 in Wuhe County from 2009 to 2011, and the incidence showed a downtrend.

[Recombinant expression and immunologic identification of bradyzoite-specific antigen BSR4 of Toxoplasma gondii].

Objective: To express the recombinant BSR4 protein of Toxoplasma gondii Prugniaud (PRU) strain and study its immunologic characteristics.

Methods: The recombinant plasmid pET28a(+)-BSR4 was transformed into E. coli BL21, followed by expression of BSR4 induced by IPTG and its purification.

[Effects of excreted/secreted antigens of Toxoplasma gondii on CD4+ CD25+ Foxp3+ T cells and NK cells of melanoma-bearing mice].

Objective: To explore the effects of excreted/secreted antigens (ESA) of Toxoplasma gondii on CD4+CD25+ Foxp3+ T cells and NK cells of melanoma-bearing mice, as well as the tumor growth.

Methods: B16F10 (denoted B16) tumor cells were cultured in complete medium and maintained by serial passage in vitro. The 2x 10(5) B16 tumor cells were injected into the right flank of the mouse to establish the tumor-bearing mice model.