Comparative proteomic and transcriptomic analysis of testicular tissue of yaks with or without cryptorchidism.

Yak is a large plateau mammal with low reproduction rate, while cryptorchidism is a major reproductive disorder associated with infertility in highland yaks. To better understand the occurrence of cryptorchidism and its regulatory mechanisms in yaks, we conducted a multi-omic analysis, and screened a total of 4456 differentially expressed genes (DEGs) and 332 differentially expressed proteins (DEPs) between normal and unilateral cryptorchid testes of yaks using high-throughput transcriptome sequencing (RNA-seq) and Tandem Mass Tag (TMT)-based proteomics techniques, with testes from yaks with cryptorchidism as the target. Enrichment analysis revealed that the DEGs were associated with cell growth, sperm motility, immune regulation, and intercellular tight junctions, and were mainly enriched in pathways related to cell differentiation; amino acid, sugar, and lipid metabolism; cell adhesion, and hypoxia tolerance.

The ferroptosis of sertoli cells inducing blood-testis barrier damage is produced by oxidative stress in cryptorchidism.

Oxidative Stress (OS) is the main cause of damage to the Blood-Testis Barrier (BTB) in cryptorchidism, which seriously endangers male reproductive health. It is well known that the OS induced ferroptosis is an important cause of dysfunction in the body. However, it is still unknown whether BTB damage in cryptorchidism leads to ferroptosis of Sertoli cells.

Analysis of Histochemical Characteristics of Submandibular Gland of the Bactrian Camel.

The ultrastructure of submandibular gland (SMG) of Bactrian camels was observed by a transmission electron microscope. Routine HE staining, special staining combined with immunohistochemistry, and immunofluorescence techniques were used to study the histochemical characteristics of the submandibular gland and the localisation and distribution characteristics of epidermal growth factor (EGF) and epidermal growth factor receptor (EGFR). HE results showed that the submandibular gland of Bactrian camels was composed of mixed serous and mucinous acini glands.

Distribution characteristics and morphological comparison of telocytes in the aortic bulb and myocardium of yak heart.

Background: Telocytes (TCs) are small interstitial cells that extend into multiple bead-like protrusions called telopodes (TPs). TCs are widely found in many tissues and organs, form connections with almost all types of cardiomyocytes, and participate in regulating cardiac microenvironment homeostasis.

Methods: In this study, transmission electron microscopy combined with special staining techniques (Gomori's, Masson's trichrome, and toluidine blue staining) were used to analyse the ultrastructure, distribution, and cytochemical characteristics of TCs in yak hearts.

Prevalence of malnutrition among adult inpatients in China: a nationwide cross-sectional study.

Malnutrition substantially contributes to adverse clinical outcomes. However, no national survey has been conducted to characterize its epidemiology in hospital settings in China. We conducted the China Nutrition Fundamental Data 2020 project among a multistage stratified cluster sample of adult inpatients from 291 study sites across 30 provinces, autonomous regions and municipalities (except for Hong Kong, Macao, Taiwan Province, and the Xizang Autonomous Region, please see MATERIALS AND METHODS for details of the causes) of China to generate reliable data on the prevalence of malnutrition and explore the associated risk factors.

Predictive value of geriatric nutritional risk indexes for hospital readmission and mortality in older patients.

The Geriatric Nutritional Risk Index (GNRI) plays a crucial role in assessing nutritional status and predicting clinical outcomes in older patients. This study explores the GNRI as a prognostic tool for clinical outcomes in older inpatients. Spanning from August 2013 to December 2020, the research involved 573 older patients at Tianjin Third Central Hospital, China.

Morphological identification and distribution comparison of telocytes in pituitary gland between normal and cryptorchid yaks.

Background: Telocytes (TCs) is a novel type of interstitial cells in many mammals organs, which participate in the organizational metabolism, mechanical support, immunomodulation and other aspects. The aim of this study was to explore the organizational chemical characteristics of TCs in pituitary gland and their changes in cryptorchid yaks.

Methods: Transmission electron microscopy (TEM), toluidine blue staining, immunofluorescence, qRT-PCR, and Western blotting may enable us to understand TCs distribution characteristics and biological functions.

Comparative genomic analysis of pathogenic factors of Listeria spp. using whole-genome sequencing.

Listeria monocytogenes is an important foodborne pathogen known for causing listeriosis. To gain insights into the pathogenicity, genetic characterization, and evolution of various Listeria species, in vitro cell adhesion and invasion ability assays and whole-genome sequencing were performed using four Listeria strains isolated from livestock and poultry slaughterhouses. The four Listeria strains exhibited adhesion and invasion abilities in Caco-2 and RAW264.

Morphological characteristics and distribution identification of telocytes in Tibetan sheep testis and epididymis.

Telocytes (TCs) are a type of stromal cell discovered in the various organs of different animals and have many potential functions, including angiogenesis, signalling, and substance transport. However, the TCs have not been detected in the testis or epididymis of Tibetan sheep. This study investigated the position, characteristics, and distribution of TCs in the testis and epididymis of Tibetan sheep using transmission electron microscopy (TEM), toluidine blue staining, immunohistochemistry, and double immunofluorescence to elucidate their possible functions.

Histological study of Bactrian camel cryptorchidism and expression of immunoglobulin λ light chain in the testicular and epididymis of cryptorchid Bactrian camel.

Lambda light chains (λ-LCs) are frequently responsible for triggering the activation of inflammatory factors in autoimmune disorders, and an increase in their levels will cause various pathological changes in serum. The aim of this study was to determine the histological differences between the epididymis and testis of normal and cryptorchid Bactrian camels and the differences in λ-LC expression in the epididymis and testis of normal and cryptorchid Bactrian camels. Haematoxylin and eosin (H&E) staining was used to examine the pathological changes in cryptorchidism.

Spatiotemporal Pattern Evolution of Food and Nutrient Production in China.

Ensuring food and nutrient supply is a crucial aspect of achieving food safety. With rapid population growth, urbanization, and social and economic development, the challenges related to China's food and nutrient production have become increasingly prominent. This paper analyzed the characteristics of the spatiotemporal pattern evolution of food and nutrient production in China from 1995 to 2020, utilizing the conversion of various food nutrients and the establishment of a gravity center model.

The spatiotemporal patterns and network characteristics of emissions embodied in the international trade of livestock products.

Livestock production is greenhouse gas (GHG) emission intensive, and thus the increasing international trade of livestock products in recent decades has resulted in increased embodied emissions. Considering the varying emission intensity in production in different countries and the expected further increase in livestock product trade in the future, it becomes crucial to understand the spatial and temporal dynamics of such embodied GHG emissions for climate change mitigation in the livestock sector. In this study, we aimed to address such gaps and analyzed the spatiotemporal patterns and network characteristics of GHG emissions embodied in the international trade of seven major categories of livestock products among 228 world economies during 1986-2017.

Characterization and Preliminary Application of Phage Isolated From .

(LM) is one of the four major foodborne bacteria that cause bacteremia and meningitis. To explore the control of listeriosis with natural phages, we used the double-layer agar plate method to isolate LM from slaughterhouse sewage and designated LP8. The result of electron microscopy indicated that the phage belonged to the family of .

Identification and validation of a miRNA-based prognostic signature for cervical cancer through an integrated bioinformatics approach.

Cervical cancer is the fourth most common cancer in women worldwide. Increasing evidence has shown that miRNAs are related to the progression of cervical cancer. However, the mechanisms that affect the prognosis of cancer are still largely unknown.

Nutritional assessment and risk factors associated to malnutrition in patients with esophageal cancer.

Introduction: Esophageal cancer is the fourth most common cause of cancer death in China. Patients with esophageal cancer are more likely to suffer from malnutrition. The purpose of this study is to assess nutritional status of patients with esophageal cancer from multiple perspectives and analyze the risk factors.

Nutritional status and survival of 8247 cancer patients with or without diabetes mellitus-results from a prospective cohort study.

Background: The number of cancer patients with diabetes mellitus (DM) is steadily rising. Little is known about the nutritional status of this population. This study characterized the nutritional status and survival of cancer patients with diabetes compared with those without diabetes.

The value of grip test, lysophosphatidlycholines, glycerophosphocholine, ornithine, glucuronic acid decrement in assessment of nutritional and metabolic characteristics in hepatitis B cirrhosis.

The liver is essential for the regulation of energy, protein and amino acids, as well as in other aspects of metabolism. To identify efficient indexes for evaluation of nutritional status and metabolic characteristics during different Child-Pugh stages of hepatitis B cirrhosis, 83 patients and 35 healthy individuals were enrolled in our study. We found that grip strength, triceps skinfold thickness (TSF), body fat and skeletal muscle of the patients were reduced compared to the control group (P<0.

Cyprinus carpio Decoction Improves Nutrition and Immunity and Reduces Proteinuria through Nephrin and CD2AP Expressions in Rats with Adriamycin-Induced Nephropathy.

Cyprinus carpio decoction (CCD) is a well-known Chinese food medicine formula, accepted widely as a useful therapy in preventing edema and proteinuria caused by renal disease. However, the mechanism underlying this effect remains unclear. The current study investigated the potential mechanism of CCD in alleviating nephropathy induced by adriamycin (ADR) in rats.

[Effects of selenium and zinc on the proliferation of human esophageal cancer cell line studied by serophysiology].

Objective: The effect of serum from rats supplemented with selenium and zinc on the proliferation of human esophageal cancer cell line Eca109 was observed by serophysiology.

Methods: Rats were randomly divided into seven groups. Eight rats in each group were fed with basic feeds (deprived of both selenium and zinc).

[Application of total nutrient admixture in acute hepatic failure rats].

Objective: To study the therapeutic efficacy of total nutrition admixture (TNA) containing 30.6% BCAA, MCT/LCT, glucose, vitamin, electrolytes in rat with acute hepatic failure (AHF).

Method: 30 Wistar rats were randomly divided into 4 groups: Normal control, AHF control, Fat-free nutrient admixture group, TNA group.

[Investigation on nutritional intakes for hospitalized children with blood disease].

Objective: To investigate the diet and nutritional status of hospitalized children with blood disease in order to provide nutritional guidelines.

Methods: The patients' daily dietary intakes, including breakfast, lunch, dinner and additional meals, were recorded in detail for seven consecutive days. The intake amount of various nutrients was calculated using the dietary database.