LncRNA A2ml2 inhibits fatty liver hemorrhage syndrome progression and function as ceRNA to target LPL by sponging miR-143-5p.

Fatty liver hemorrhage syndrome (FLHS) is the most common metabolic diseases in laying hens during the late-laying period, and it causes a significant economic burden on the poultry industry. The competing endogenous RNA plays crucial roles in the occurrence and development of fatty liver. Based on the previously constructed lncRNA-miRNA-mRNA networks, we selected the axis of ENSGALT00000079786-LPL-miR-143-5p for further study to elucidate its mechanistic role in development of fatty liver.

PINCH-1 promotes tumor growth and metastasis by enhancing DRP1-mediated mitochondrial fission in head and neck squamous cell carcinoma.

Purpose: Abnormal expression of PINCH-1 has been observed in various types of human cancers. However, the clinical importance and mechanism underlying its role in head and neck squamous cell carcinoma (HNSCC) is yet to be fully elucidated.

Methods: This study evaluated the expression of PINCH-1 in HNSCC samples through immunohistochemical staining and Western blotting.

Machine learning model-based prediction of postpancreatectomy acute pancreatitis following pancreaticoduodenectomy: A retrospective cohort study.

Background: The International Study Group of Pancreatic Surgery has established the definition and grading system for postpancreatectomy acute pancreatitis (PPAP). There are no established machine learning models for predicting PPAP following pancreaticoduodenectomy (PD).

Aim: To explore the predictive model of PPAP, and test its predictive efficacy to guide the clinical work.

Integrating short-read and long-read single-cell RNA sequencing for comprehensive transcriptome profiling in mouse retina.

The vast majority of protein-coding genes in the human genome produce multiple mRNA isoforms through alternative splicing, significantly enhancing the complexity of the transcriptome and proteome. To establish an efficient method for characterizing transcript isoforms within tissue samples, we conducted a systematic comparison between single-cell long-read and conventional short-read RNA sequencing techniques. The transcriptome of approximately 30,000 mouse retina cells was profiled using 1.

Spermine accumulation via spermine synthase promotes tumor cell proliferation in head and neck squamous cell carcinoma.

Background: Head and neck squamous cell carcinoma (HNSCC) is among the most aggressive malignancies, underscoring the need for early diagnosis to improve patient outcomes. Tumor-derived exosomes, which can be non-invasively obtained and reflect the metabolic state of tumors in real-time, are under increasing investigation for their diagnostic potential. Herein we analyzed metabolite differences in exosomes, serum, and tissues from patients with HNSCC to identify potential diagnostic biomarkers of clinical relevance.

Microbial welan gum production, chemistry and applications: A review.

Welan gum, an extracellular polysaccharide produced by the Sphingomonas sp., has attracted considerable interest due to its distinctive properties and promising applications in adhesion, thickening, suspension, emulsification, stabilization, lubrication, and film formation. However, several unresolved issues related to welan gum, particularly its low biosynthesis efficiency, have hindered its widespread industrial application.

Molecular chelating and shielding operation for organic photoelectrochemical transistor detection of chlorpyrifos.

Chlorpyrifos (CPF), as one of the four major insecticides for pest control, is widely used in agricultural production, but its continuous accumulation has caused serious pollution to the environment. In this paper, we developed a novel organic photoelectrochemical transistor (OPECT) sensor for the CPF detection using a BiOI@BiS photogate. The molecular chelating of CPF with surface Bi(III) ions would induce the shielding effect that altered the OPECT response.

Gastric-type endocervical adenocarcinoma, superficial myofibroblastoma, sex cord-stromal tumors, and HSIL in Peutz-Jeghers syndrome: a rare case report, genetic characterization, and review of literature.

Peutz-Jeghers syndrome (PJS) is characterized by an increased risk of gynecologic tumors. Gastric-type endocervical adenocarcinoma (GEA) is a rare non-human papillomavirus (HPV)-related tumor. We reported an uncommon case of a 39-year-old woman with PJS who developed GEA, superficial cervical vaginal myofibroblastoma, sex cord-stromal tumors with annular tubules of the ovaries, and cervical and vaginal high-grade squamous interepithelial neoplasia (HSIL).

Preparation of Au-modified metal organic framework nanozyme with tunable catalytic activity used for diabetic wound healing.

Nanozymes with tunable catalytic activity have attracted attention in the field of biomedicine. Bacterial infections are the main causes of delayed or chronic wound healing. Therefore, antibacterial nanoplatforms with tunable enzymatic activity are urgently required for diabetic wound healing.

Triple-Functional Smart Organic Molecules Enable Self-Enhancement Modulation of Organic Photoelectrochemical Transistor.

Organic photoelectrochemical transistor (OPECT) has undergone significant advancements, enabling an effective synergy between organic electronics and photoelectrochemistry, contributing to opto-logic gates, neuromorphic emulation, and biological detection. However, feasible OPECT operation is still quite limited and the associated technology is evolving. This study introduces a self-enhancement OPECT operation facilitated by triple-functional stimuli-responsive organic molecules (SROM).

Functional dissection of three pollen-side quantitative trait loci against multiple stylar unilateral incompatibility mechanisms in Solanum pennellii LA0716.

In Solanum pennellii LA0716, three stylar UI (sui) factors and one pollen UI (pui) factor were shown to be involved in S-RNase-independent unilateral incompatibility (UI). However, additional pui factor(s) and the antagonistic relationships among pui and sui factors remain to be investigated. Quantitative trait loci (QTL) mapping, functional and genetic analysis of LA0716-based crosses, and integrated multi-omics data are used to identify pui QTLs and functionally dissect pui QTLs from various types of stylar UI.

Biotransformation of baicalin and glycyrrhizic acid using immobilized FeO@Chitosan@β-glucuronidase.

Unlabelled: β-Glucuronidase can hydrolyze β-glucuronic acid-containing glycosides, such as baicalin and glycyrrhizic acid. In this study, the β-glucuronidase gene from was cloned and expressed in . The resulting recombinant protein, designated LrhGUS, exhibited a molecular weight of approximately 72 kDa.

Divergence in cellular markers observed in single-cell transcriptomics datasets between cultured primary trabecular meshwork cells and tissues.

The trabecular meshwork within the outflow apparatus is critical in maintaining intraocular pressure homeostasis. In vitro studies employing primary cell cultures of the human trabecular meshwork (hTM) have conventionally served as surrogates for investigating the pathobiology of TM dysfunction. Despite its abundant use, translation of outcomes from in vitro studies to ex vivo and/or in vivo studies remains a challenge.

Pathogenesis and regenerative therapy in vitiligo and alopecia areata: focus on hair follicle.

Vitiligo is an autoimmune disease characterized by the loss of functional melanocytes in the hair follicles and epidermis, leading to white patches on the skin and mucous membranes. Alopecia areata (AA) is a common immune-mediated condition in which autoimmune attack on hair follicles cause non-scarring hair loss. Both diseases significantly impact patients's physical and mental health.

Improved multi-input parameter optimization method for camera colorimetric characterization.

In order to improve the accuracy of camera colorimetric characterization, a multi-input parameter optimization method was proposed in this paper. The input parameters of the traditional camera characterization method were generally RGB values; in the proposed method, the luminance parameter L was introduced in addition to RGB values, and the four-input parameters of RGBL were used as input parameters for the conversion model. In the experiment, 549 colors were uniformly selected from the Munsell Book of Color (Matte Edition), and the RGBL values and corresponding CIEXYZ values of the selected colors were measured by a spectroradiometer and three cameras, including an imaging luminance meter, respectively.

Modular Engineering of Lysostaphin with Significantly Improved Stability and Bioavailability for Treating MRSA Infections.

Methicillin-resistant (MRSA) is a refractory pneumonia-causing pathogen due to the antibiotic resistance and the characteristics of persisting inside its host cell. Lysostaphin is a typical bacteriolytic enzyme for degrading bacterial cell walls via hydrolysis of pentaglycine cross-links, showing potential to combat multidrug-resistant bacteria. However, there are still grand challenges for native lysostaphin because of its poor shelf stability and limited bioavailability.

Tröger's base-embedded macrocycles with chirality.

The birth and development of supramolecular chemistry have heralded a new era, where macrocycles have become an irreplaceable research tool. Therefore, the construction of novel macrocycles has become a hot spot. Tröger's base (TB), as a fragment with both rigidity and chirality, promises tremendous potential in the realm of supramolecular chemistry, and its unique properties continue to motivate researchers to explore its inclusion in macrocycles.

Extracorporeal membrane oxygenation for catecholaminergic polymorphic ventricular tachycardia: a case report and literature review.

Background: Catecholaminergic polymorphic ventricular tachycardia (CPVT) is an inherited ion channelopathy characterized by a structurally normal heart sensitive to catecholamines. It primarily presents as Bidirectional ventricular tachycardia (BiVT) and is a significant cause of sudden cardiac death in children.

Case Presentation: We report our experience with central Extracorporeal Membrane Oxygenation (ECMO) therapy in a 4-year-old boy with CPVT.

Phenotypic and Genetic Heterogeneity of a Pakistani Cohort of 15 Consanguineous Families Segregating Variants in Leber Congenital Amaurosis-Associated Genes.

Background: Leber congenital amaurosis (LCA) is a congenital onset severe form of inherited retinal dystrophy (IRD) and a common cause of pediatric blindness. Disease-causing variants in at least 14 genes are reported to predispose LCA phenotype. LCA is inherited as an autosomal recessive disease.

Spatiotemporal differences induced changes in the structure and function of the gut microbiota in an endangered ungulate.

The composition and function of animal gut microbiota are shaped by various factors, among which diet is one of the major factors. Diet is affected by seasonal shifts and geographical differences, which in turn impact the host's nutritional levels. To adapt to these environmental changes, the gut microbiome often produces matching responses.

Systematic evaluation of methylation-based cell type deconvolution methods for plasma cell-free DNA.

Background: Plasma cell-free DNA (cfDNA) is derived from cellular death in various tissues. Investigating the tissue origin of cfDNA through cell type deconvolution, we can detect changes in tissue homeostasis that occur during disease progression or in response to treatment. Consequently, cfDNA has emerged as a valuable noninvasive biomarker for disease detection and treatment monitoring.

Straw Returning Methods Affects Macro-Aggregate Content and Organic Matter Content in Black Soils: Meta-Analysis and Comprehensive Validation.

Straw returning into the soil is a crucial method for boosting soil carbon levels. To research the influence of straw return practices on soil aggregates and organic matter content within the farmlands of the Northeast Black Soil Region, the objective was to clarify the varying impacts of these practices on soil carbon enhancement. In this study, 89 pertinent papers were acquired through a rigorous literature compilation.

A novel all-in-one target-powered entropy-driven dynamic DNA networks to regulate the activity of CRISPR/AsCas12a for enhanced DNA detection.

Background: The non-enzyme autonomous DNA nanodevices have been developed to detect various analytes through the programmability of Watson-Crick base pairing. Nevertheless, by comparison with enzymatic biosensors, the usage of enzyme-free DNA networks to create biosensors for testing low amounts of targets is still subject to the finite number of cycles. Besides, these biosensors still require the incorporation of other amplification strategies to improve the sensitivity, which complicates the detection workflow and lacks of a uniform compatible system to respond to the target in one pot.

Development and validation of the self-report symptom inventory of immune-related adverse events in patients with lung cancer.

Objective: This study aims to develop and validate the Self-Report Symptom Inventory of immune-related Adverse Events in Patients with Lung Cancer (SRSI-irAEs-LC) to allow for systematic assessment of symptomatic irAEs in patients with lung cancer treated with programmed cell death protein 1 (PD-1)/programmed death-ligand 1 (PD-L1) immune checkpoint inhibitors (ICIs).

Methods: A sequential two-phase mixed-methods study was conducted. In phase I, a draft version of the SRSI-irAEs-LC was constructed through item generation and draft inventory construction.