Optimized whole-cell depolymerization of polyethylene terephthalate to monomers using engineered Clostridium thermocellum.

Polyethylene terephthalate (PET) is a widely produced thermoplastic derived from fossil fuels, and its accumulation and improper waste disposal pose significant environmental concerns. Innovative bio-based recycling technologies have evolved in recent years, offering viable solutions to PET waste-related challenges. While the enzyme-based PET recycling technology utilizing free thermophilic enzymes has already been commercialized, related whole-cell recycling approaches are still in the early stages of research.

The Complete Mitochondrial Genome of Nephropsis grandis: Insights into the Phylogeny of Nephropidae Mitochondrial Genome.

The systematic phylogeny of Pleocyemata species, particularly within the family Nephropidae, remains incomplete. In order to enhance the taxonomy and systematics of Nephropidae within the evolutionary context of Pleocyemata, we embarked upon a comprehensive study aiming to elucidate the phylogenetic position of Nephropsis grandis. Consequently, we determined the complete mitochondrial DNA sequence for N.

Sequence comparison of the mitochondrial genomes of five caridean shrimps of the infraorder Caridea: phylogenetic implications and divergence time estimation.

Background: The Caridea, affiliated with Malacostraca, Decapoda, and Pleocyemata, constitute one of the most significant shrimp groups. They are widely distributed across diverse aquatic habitats worldwide, enriching their evolutionary history. In recent years, considerable attention has been focused on the classification and systematic evolution of Caridea, yet controversies still exist regarding the phylogenetic relationships among families.

Sequence comparison of the mitochondrial genomes of species (Caridea: Pandalidae), gene rearrangement and phylogenetic relationships of Caridea.

Background: Despite the Caridean shrimps' vast species richness and ecological diversity, controversies persist in their molecular classification. Within Caridea, the Pandalidae family exemplifies significant taxonomic diversity. As of June 25, 2023, GenBank hosts only nine complete mitochondrial genomes (mitogenomes) for this family.

Characterization of C carotenoid and identification of its biosynthetic gene cluster in AM1.

species, the representative bacteria distributed in phyllosphere region of plants, often synthesize carotenoids to resist harmful UV radiations. is known to produce a carotenoid pigment and recent research revealed that this carotenoid has a C backbone. However, its exact structure remains unknown.

Comparison of Mitochondrial Genome Sequences between Two Species of the Family Palaemonidae (Decapoda: Caridea): Gene Rearrangement and Phylogenetic Implications.

To further understand the origin and evolution of Palaemonidae (Decapoda: Caridea), we determined the mitochondrial genome sequence of and . The entire mitochondrial genome sequences of these two species encompassed 37 typical genes, including 13 protein-coding genes (PCGs), 2 ribosomal RNA genes (rRNAs), and 22 transfer RNA genes (tRNAs), and a control region (CR). The lengths of their mitochondrial genomes were 15,744 bp () and 15,735 bp (), respectively.

Label-free monitoring of spatiotemporal changes in the stem cell cytoskeletons in time-lapse phase-contrast microscopy.

Investigation of the dynamic structural changes in the actin cytoskeleton during cell migration provides crucial information about the physiological conditions of a stem cell during in-vitro culture. Here we proposed a quantitative analytical model associated with texture extraction with cell tracking techniques for in situ monitoring of the cytoskeletal density change of stem cells in phase-contrast microscopy without fluorescence staining. The reliability of the model in quantifying the texture density with different orientation was first validated using a series of simulated textural images.

Local neural-network-weighted models for occurrence and number of down wood in natural forest ecosystem.

The natural forest ecosystem has been affected by wind storms for years, which have caused several down wood (DW) and dramatically modified the fabric and size. Therefore, it is very important to explain the forest system by quantifying the spatial relationship between DW and environmental parameters. However, the spatial non-stationary characteristics caused by the terrain and stand environmental changes with distinct gradients may lead to an incomplete description of DW, the local neural-network-weighted models of geographically neural-network-weighted (GNNWR) models are introduced here.

Study on the Estimation of Forest Volume Based on Multi-Source Data.

We performed a comparative analysis of the prediction accuracy of machine learning methods and ordinary Kriging (OK) hybrid methods for forest volume models based on multi-source remote sensing data combined with ground survey data. Taking , , and plantations in Mengjiagang forest farms as the research object, based on the Chinese Academy of Forestry LiDAR, charge-coupled device, and hyperspectral (CAF-LiTCHy) integrated system, we extracted the visible vegetation index, texture features, terrain factors, and point cloud feature variables, respectively. Random forest (RF), support vector regression (SVR), and an artificial neural network (ANN) were used to estimate forest volume.

Effect of Mechanical Heterogeneity on Strain and Stress Fields at Crack Tips of SCC in Dissimilar Metal Welded Joints.

The crack tip strain and stress condition are one of the main factors affecting stress corrosion cracking (SCC) behaviors in the dissimilar metal welded joint of the primary circuit in the pressurized water reactor. The mechanical property mismatch of base metal and weld metal can significantly affect the stress and strain condition around the crack tip. To understand the effect of different weld metals on strain and stress fields at SCC crack tips, the effects of strength mismatch, work hardening mismatch, and their synergy on the strain and stress field of SCC in the bi-material interface, including plastic zone, stress state, and corresponding -integral, are investigated in small-scale yielding using the finite element method.

Role of HPV status and PD-L1 expression in prognosis of laryngeal squamous cell carcinoma.

Purpose: Human papillomavirus (HPV) infection has been recognized as a cause of head and neck squamous cell carcinomas (HNSCC). Laryngeal squamous cell carcinoma (LSCC) is one of the most common pathologic types of HNSCC. Clinical trials show that there are differences in response to immunotherapy according to HPV status.

Histone deacetylase inhibitor valproic acid attenuates high glucose‑induced endoplasmic reticulum stress and apoptosis in NRK‑52E cells.

Previous studies have demonstrated that valproic acid (VPA), a histone deacetylase inhibitor, alleviates diabetic nephropathy (DN). However, the biological mechanisms underlying this protective effect remains unclear. This study aimed to investigate the effects of histone deacetylase inhibitor VPA on hyperglycemic induction of NRK‑52E cell ERS and apoptosis.

Quantitative Bioimage Analysis of Passaging Effect on the Migratory Behavior of Human Mesenchymal Stem Cells During Spheroid Formation.

The quality of stem cells obtained through serial subcultivation is the pivotal factor determining the therapeutic effectiveness of regenerative medicine. However, an effective quality monitoring system for cell culture is yet to be established. Detailed parameter studies of the migratory behavior of stem cells at different passages may provide insight into the deterioration of stemness.

A dual-label time-resolved fluorescence immunoassay for the simultaneous determination of carcinoembryonic antigen and squamous cell carcinoma antigen.

Among women worldwide, cervical cancer is the second-most common cancer, and cervical smears and DNA detection have low sensitivity or are too expensive. The concentrations of carcinoembryonic antigen (CEA) and squamous cell carcinoma (SCC) in the serum were detected using a sandwich immunoassay. The CEA and SCC in the serum were captured by anti-CEA and anti-SCC antibodies.

Prognostic value of human papillomavirus infection and p53, p16, epidermal growth factor receptor and p34 expression in patients with salivary adenoid cystic carcinoma.

The aim of this study was to explore the correlation between HPV infection, p53, p16, epidermal growth factor receptor (EGFR), p34 protein expression and prognosis in patients with adenoid cystic carcinoma of salivary gland. Totally 78 cases of adenoid cystic carcinoma of salivary gland specimens were selected from January 1, 2004 to December 31, 2013 in Tangshan Union Hospital. PCR-reverse dot blot hybridization was used to detect infection of human papilloma virus (HPV), and SP immunohistochemical method was adopted to detect the expression of p53, p16, EGFR and p34 protein in the carcinoma tissues.

Expression of p27 and PTEN and clinical characteristics in early laryngeal squamous cell carcinoma and their correlation with recurrence.

Laryngeal squamous cell carcinoma is a common malignant tumor of otolaryngeal region. At present, effective treatment of laryngeal squamous cell carcinoma still depends on surgery and radiotherapy. In recent years, application of CO2 laser resection in the treatment of stage T1 glottic carcinoma can remove the tumor completely and reduce the injury of laryngeal tissues.

Over-expression of p53, p21 and Cdc2 in histologically negative surgical margins is correlated with local recurrence of laryngeal squamous cell carcinoma.

The aim of this study was to explore the relationship between the expression of p53, p21 and Cdc2 in the early laryngeal cancer with negative pathological margins and its local recurrence. During 2004-2010, a total of 85 patients with early laryngeal cancer were selected in Tangshan Union Hospital, Hebei, China, and immunohistochemical method was used to detect the expression of p53, p21 and Cdc2 in the negative pathological margin tissues. All patients were followed up for two years to collect pathological data for evaluating the survival and tumor recurrence.

Down-regulation of microRNA-181b is a potential prognostic marker of non-small cell lung cancer.

The aim of this study was to investigate the clinical significance of microRNA-181b (miR-181b) expression in non-small cell lung cancer (NSCLC). MiR-181b expression in 126 pairs of surgically removed NSCLC tissues and their corresponding normal lung tissues was measured by real-time quantitative RT-PCR assay. Additionally, the correlation of miR-181b expression with clinicopathological factors or prognosis of patients was analyzed.

[Study of SOD and PON-1 expression in type 2 diabetes mellitus nephropathy and its clinical significance].

Aim: To investigate the clinical value of superoxide dismutase (SOD) and paraoxonase-1 (PON-1) in type 2 diabetes mellitus (T₂DM) nephropathy.

Methods: 80 cases of T₂DM patients were divided into T₂DM nephropathy group (ND group, 40 cases), and non-T₂DM nephropathy group (non-ND group, 40 cases), the SOD, PON-1 levels and biochemical levels of the two group were measured, the HOMA-IR was recorded.

Results: The SOD and PON-1 levels of the ND group were lower than the non-ND group, compared the difference was significant (P < 0.

[Relationship between expression of caveolin-1 and pERK1/2 and prognosis in non-small cell lung cancer].

Objective: To study the relationship between expression of caveolin-1 (Cav-1) and pERK1/2 and prognosis in non-small cell lung cancer (NSCLC).

Methods: Cav-1 and pERK1/2 protein expression was assessed by immunohistochemistry in samples obtained from 160 patients with NSCLC and 20 patients with normal lung tissue.

Results: Normal bronchial and alveolar epithelial cells were positive for Cav-1 (membranous and cytoplasmic staining patterns).