snRNA-Seq and Spatial Transcriptome Reveal Cell-Cell Crosstalk Mediated Metabolic Regulation in Porcine Skeletal Muscle.

Background: Cell-cell crosstalk between myogenic, adipogenic and immune cells in skeletal muscle to regulate energy metabolism and lipid deposition has received considerable attention. The specific mechanisms of interaction between the different cells in skeletal muscle are still unclear.

Methods: Using integrated analysis of snRNA-seq and spatial transcriptome, the gene expression profile of longissimus dorsi (LD) muscle was compared between adult Taoyuan black (TB, obese, native Chinese breed) and Duroc (lean) pigs.

Food waste used as a resource can reduce climate and resource burdens in agrifood systems.

Global food loss and waste continues to increase despite efforts to reduce it. Food waste causes a disproportionally large carbon footprint and resource burdens, which require urgent action to transition away from a disposal-dominated linear system to a circular bioeconomy of recovery and reuse of valuable resources. Here, using data from field-based studies conducted under diverse conditions worldwide, we found collective evidence that composting, anaerobic digestion and repurposing food waste to animal feed (re-feed) result in emission reductions of about 1 tCOe t food waste recycled compared with landfill disposal.

Effects of peroxidized lipids on intestinal morphology, antioxidant capacity and gut microbiome in piglets.

This study investigated the effect of peroxidized lipids on piglets' growth performance, intestinal morphology, inflammatory reactions, oxidative stress in the liver, duodenum, jejunum, ileum, and colon, and ileal microbiota. Twenty piglets (Duroc × [Landrace × Yorkshire]; age = 21 d old, BW = 6.5 ± 1 kg) were randomly assigned to two groups with 10 replicates per group and one piglet per replicate.

Effect of Different Ratios of α-Lactalbumin to β-Casein in Infant Formula on True Ileal Digestibility and Intestinal Morphology of Suckling Piglets.

Breast milk (BM) is the optimal nutrition source for infants, providing highly digestible proteins. In contrast, the digestibility of proteins in infant formula (IF) varies, influenced by differences in proteins such as α-lactalbumin (α-La) and β-casein (β-CN). This study aimed to evaluate the true ileal digestibility (TID) of amino acids (AAs) in BM and IF with varying α-La and β-CN contents using neonatal piglets.

Effects of Fish Bone Meal Supplementation on Growth Performance, Blood Immunity, Intestinal Morphology, and Gut Microbiota in Laying Hens.

The poultry industry relies heavily on soybean meal to feed egg-laying chickens, but its high cost and price instability necessitate the exploration of more affordable and reliable alternatives. Fish bone meal, a nutritious byproduct from fish processing, offers high-quality protein and essential minerals, making it a promising addition to poultry diets. In this study, 240 Hy-Line Brown laying hens (31 weeks old, average weight 1.

Father Phubbing and Adolescents' Depressive Symptoms: The Roles of Relationship Satisfaction and Need to Belong.

Recent research indicates that parental phubbing is a risk factor for adolescents' depressive symptoms; however, limited research has examined the association between father phubbing and adolescents' depressive symptoms. The present study tested the association between father phubbing and adolescents' depressive symptoms. Furthermore, the mediating and moderating effects underlying this link were examined.

A Brief Review of Aptamer-Based Biosensors in Recent Years.

Aptamers have recently become novel probes for biosensors because of their good biocompatibility, strong specificity, and high sensitivity. Biosensors based on peptides or nucleic acid aptamers are used in implantable and wearable devices owing to their ease of synthesis and economic efficiency. Simultaneously, amphoteric ionic peptides are being explored as antifouling layers for biosensors resistant to interference from extraneous proteins in serum.

A low-protein soybean-free diet improves carcass traits and meat quality and modulates the colonic microbiota in Daweizi pigs.

Introduction: Soybean meal is an excellent protein source and is widely used in pig feed. However, the Americas account for more than 80% of global soybean production, so European and Asia swine production largely depends on soybean imports. The use of safe and functional unconventional feed sources can effectively alleviate worldwide protein shortage problems.

Adenosine Monophosphate Improves Lipolysis in Obese Mice by Reducing DNA Methylation via ADORA2A Activation by Ecto-5'-Nucleotidase (CD73).

The previous work discovers the potential of adenosine monophosphate (AMP) to alleviate obesity-related metabolic diseases, but the underlying molecular mechanisms remain incompletely understood. Here, AMP is confirmed to enhance white fat decomposition and improve abnormal glucose and lipid metabolism in mice fed with a high-fat (HF) diet. Mechanically, AMP is converted to adenosine (ADO) through ecto-5'-nucleotidase (CD73), and adenosine A2A receptor (ADORA2A) signaling activation is involved in the down-regulation of methylation in white adipose tissue, thereby reducing the hormone-sensitive lipase (HSL) methylation level and promoting HSL transcription and white fat decomposition.

Glutamate increases the lean percentage and intramuscular fat content and alters gut microbiota in Shaziling pigs.

This study aimed to explore the effects of glutamate (Glu) supplementation on the growth performance, carcass traits, meat quality, composition of amino acids and fatty acids in the longissimus dorsi muscle, and the colonic microbial community of Shaziling pigs. A total of 48 healthy male Shaziling pigs (150 d, 31.56 ± 0.

Ningxiang pig-derived lactobacillus reuteri modulates host intramuscular fat deposition via branched-chain amino acid metabolism.

Background: Gut microbiota has been extensively demonstrated to modulate host lipid metabolism. Higher intramuscular fat (IMF) accumulation in Chinese indigenous breed pigs is associated with their special gut microbiota structure. However, the specific microbes and metabolic pathways responsible for lipid deposition are still poorly understood.

Odd-Chain Fatty Acid-Enriched Fats Improve Growth and Intestinal Morphology and Function in Milk Replacer-Fed Piglets.

Background: The intestinal development and nutritional needs of piglets after birth are similar to those of human infants.

Objectives: This study aimed to investigate the effect of different forms of odd-chain fatty acids (OCFAs) on the growth and intestinal morphology and function of milk replacer-fed piglets, as a model for human infants.

Methods: Forty 7-d-old piglets from 8 sows were randomly assigned into 5 groups (n = 8, each from a different litter) and fed sow milk or milk replacers supplemented with different kinds of fats (control fats, docosahexaenoic acid algal oil-, OCFA algal oil-, and OCFA-enriched fats) for 21 d.

Aspartate Metabolism-Driven Gut Microbiota Dynamics and RIP-Dependent Mitochondrial Function Counteract Oxidative Stress.

Aspartate (Asp) metabolism-mediated antioxidant functions have important implications for neonatal growth and intestinal health; however, the antioxidant mechanisms through which Asp regulates the gut microbiota and influences RIP activation remain elusive. This study reports that chronic oxidative stress disrupts gut microbiota and metabolite balance and that such imbalance is intricately tied to the perturbation of Asp metabolism. Under normal conditions, in vivo and in vitro studies reveal that exogenous Asp improves intestinal health by regulating epithelial cell proliferation, nutrient uptake, and apoptosis.

Abnormal adipose tissue-derived microbes drive metabolic disorder and exacerbate postnatal growth retardation in piglet.

Postnatal growth retardation (PGR) frequently occurs during early postnatal development of piglets and induces high mortality. To date, the mechanism of PGR remains poorly understood. Adipose tissue-derived microbes have been documented to be associated with several disorders of metabolism and body growth.

A dynamic optimization of soil phosphorus status approach could reduce phosphorus fertilizer use by half in China.

Sustainable phosphorus (P) management is essential for ensuring crop production while avoiding environmental damage and the depletion of phosphate rock reserves. Despite local demonstration scale successes, the widespread mobilization of smallholder farmers to adopt sustainable management practices remains a challenge, primarily due to the associated high costs and complicated sampling. Here, we propose a dynamic optimization of soil P status (DOP) approach aimed at managing long-term soil P status within the range of agronomic and environmental soil P thresholds, which facilitates the precise determination of optimal P application rates without the need for frequent soil testing.

Effects of dietary amylose/amylopectin ratio on antioxidant ability and amino metabolism in the liver of weaned piglets undergoing feed transition and challenged with lipopolysaccharide.

To find out whether dietary amylose/ amylopectin ratio (DAR) could attenuate injury in lipopolysaccharide (LPS)-challenged piglets, sixty male weaned piglets (Duroc × Landrace × Yorkshire, 21 days old, 6.51 ± 0.64 kg) were allotted to 5 dietary treatments with 12 cages per treatment, and fed ad libitum with diets different in DAR (0.

Metabolome and RNA-seq reveal discrepant metabolism and secretory metabolism profile in skeletal muscle between obese and lean pigs at different ages.

Metabolites and metabolism-related gene expression profiles in skeletal muscle change dramatically under obesity, aging and metabolic disease. Since obese and lean pigs are ideal models for metabolic research. Here, we compared metabolome and transcriptome of Longissimus dorsi (LD) muscle between Taoyuan black (TB, obese) and Duroc (lean) pigs at different ages.

Near-infrared fluorogenic RNA for in vivo imaging and sensing.

Fluorogenic RNA aptamers have various applications, including use as fluorescent tags for imaging RNA trafficking and as indicators of RNA-based sensors that exhibit fluorescence upon binding small-molecule fluorophores in living cells. Current fluorogenic RNA:fluorophore complexes typically emit visible fluorescence. However, it is challenging to develop fluorogenic RNA with near-infrared (NIR) fluorescence for in vivo imaging and sensing studies.

Residue Regulates Immunity, Antioxidant Ability, Intestinal Barrier Function, and Microbial Structure in Weaned Piglets.

residue (AR), as the byproduct of industrial extraction of artemisinin, contains rich nutrients and active ingredients. This study was conducted to determine the effects of AR as an unconventional feed material on growth performance, immunity, and intestinal health in weaned piglets. Thirty-two piglets weaned at 21 days (7.

Isatidis root polysaccharides ameliorates post-weaning diarrhea by promoting intestinal health and modulating the gut microbiota in piglets.

This study aimed to investigate the effects of dietary isatidis root polysaccharide (IRP) on diarrhea, immunity, and intestinal health in weanling piglets. Forty healthy piglets were randomly assigned to five groups receiving varying dosages of IRP. The findings indicated that different concentrations of IRP significantly reduced diarrhea scores ( < 0.

Conference 2024: Building an innovative scientific research ecosystem for microbiome and One Health.

The Conference 2024 provides a platform to promote the development of an innovative scientific research ecosystem for microbiome and One Health. The four key components - Technology, Research (Biology), Academic journals, and Social media - form a synergistic ecosystem. Advanced technologies drive biological research, which generates novel insights that are disseminated through academic journals.

Gut protects against fat deposition by enhancing secondary bile acid biosynthesis.

Gut microbiome is crucial for lipid metabolism in humans and animals. However, how specific gut microbiota and their associated metabolites impact fat deposition remains unclear. In this study, we demonstrated that the colonic microbiome of lean and obese pigs differentially contributes to fat deposition, as evidenced by colonic microbiota transplantation experiments.

Genomic Prediction of Semen Traits in Boars Incorporating Biological Interactions.

In the context of modern pig farming, the central role of boars is underscored by large-scale centralized breeding and the widespread application of artificial insemination techniques. However, previous studies and breeding programs have focused mainly on product efficiency traits, such as growth rate, lean meat yield, and litter size, often neglecting boar semen traits. In this study, we estimated the genetic parameters and assessed the genomic prediction accuracy of boar semen traits with phenotypes evaluated from 274,332 ejections in a large population consisting of 2467 Duroc boars.