Evaluation of microchip material and surface treatment options for IEF of allergenic milk proteins on microchips.

The use of glass and PDMS microchips has been investigated to perform rapid and efficient separation of allergenic whey proteins by IEF. To decrease EOF and to limit protein adsorption, two coating procedures have been compared. The first one consists in immobilizing hydroxypropyl cellulose (HPC) and the second one poly(dimethylacrylamide-co-allyl glycidyl ether) (PDMA-AGE).

CE separation approaches for combinations of anthracyclines and taxanes.

For the first time the versatility of CE is demonstrated for the separation of different types of anticancer drugs - anthracyclines and taxanes simultaneously. The use of these drugs in combination therapy for cancer has sparked interest in the development of methods for potential application. The simultaneous analysis of anthracyclines and taxanes can significantly increase a sample throughput of a clinical laboratory.

Versatile method for electroosmotic flow measurements in microchip electrophoresis.

A novel versatile method for the determination of low or high electroosmotic mobility values in microdevices of variable microchannel design is presented. The electroosmotic flow (EOF) calculation is based on the difference between the apparent and effective mobilities of a reference compound. The proposed method uses microchip frontal electrophoresis for the determination of these mobilities.

Determination of montelukast sodium by capillary electrophoresis.

This work verifies the potential of CE in the analysis of significant impurities of montelukast sodium - an active ingredient for the treatment of bronchial asthma. Using 20 mM borate buffer pH 9.2 with 10 mM SDS and 10 mM (2-hydroxypropyl)-gamma-CD (2HP-gamma-CD) it was possible to separate montelukast and several impurities, including its cis-isomer, after exposure to light and oxygen.

Determination of association constants of inclusion complexes of steroid hormones and cyclodextrins from their electrophoretic mobility.

CE estimation of the association constants of several steroid hormones with beta-CD and gamma-CD and their hydroxypropyl derivative is presented. Estriol, 17beta-estradiol, ethynylestradiol, estrone, progesterone, mestranol and norethindrone are among the target analytes. The calculation of the cyclodextrin:analyte association constants were performed from the electrophoretic mobility values of steroids at different concentration of CDs in the run buffer.