Population sequencing of cherry accessions unravels the evolution of Cerasus species and the selection of genetic characteristics in edible cherries.

Cerasus is a subgenus of Prunus in the family Rosaceae that is popular owing to its ornamental, edible, and medicinal properties. Understanding the evolution of the Cerasus subgenus and identifying selective trait loci in edible cherries are crucial for the improvement of cherry cultivars to meet producer and consumer demands. In this study, we performed a de novo assembly of a chromosome-scale genome for the sweet cherry (Prunus avium L.

Alleviation of Glucocorticoid-Induced Osteoporosis in Rats by Ethanolic (Thunb.) Moldenke Extract.

Secondary osteoporosis is frequently due to the use of high-dose glucocorticoids (GCs). The existing strategy for managing glucocorticoid-induced osteoporosis (GIOP) is considered insufficient and remains in a state of ongoing evolution. Therefore, it is crucial to develop more precise and effective agents for the treatment of GIOP.

Comparison of two emergency cervical cerclage techniques in twin pregnancies: A retrospective cohort study matched with cervical dilation.

Objectives: This retrospective cohort study aimed to assess the efficacy of emergency cervical cerclage (ECC) performed with the combined McDonald-Shirodkar technique in twin pregnancies between 18 and 26 weeks of pregnancy with painless cervical dilation 1 to 6 cm.

Methods: A retrospective cohort study matched with the degree of cervical dilation was conducted. The study group (case group) included women with twin pregnancies undergoing combined McDonald-Shirodkar approach with cervical dilation ≥1 cm between 18 to 26 weeks of gestation at four institutions, from December 2015 to December 2022.

The complete change in bile acids and steroids in systematic metabolomics applied to the intrahepatic cholestasis of pregnancy.

Intrahepatic cholestasis of pregnancy (ICP) is a pregnancy-specific hepatobiliary disease, leading to an abnormal increase in total bile acid in the blood of pregnant women. To systematically explore the similarities and differences in metabolites and metabolic pathways among three types of biological samples from ICP women, a study of 18 ICP and 6 healthy (as a normal control) pregnant women was performed to investigate their clinical information and biochemical features. Based on validated LC-MS/MS methods 1-5 for hydrophilic and hydrophobic metabolites (molecular weight <2000 Dalton), an untargeted-metabolomic strategy was applied to 24 pregnant women to determine the metabolites from 22 serum, 15 placental and 22 urine samples.

Evolutionary and phylogenetic analyses of 11 species based on the complete chloroplast genome.

The subgenus , one of the most important groups in the genus , comprises over 100 species; however, the taxonomic classification and phylogenetic relationships of remain controversial. Therefore, it is necessary to reconstruct the phylogenetic tree for known species. Here, we report the chloroplast (cp) genome sequences of 11 species to provide insight into evolution of the plastome.

Comparative transcriptome and metabolome analyses of cherry leaves spot disease caused by .

is a necrotrophic fungal pathogen with a broad host range that causes widespread and devastating disease in sweet cherry (). We selected a resistant cultivar (RC) and a susceptible cultivar (SC) of cherry and used a combined physiological, transcriptomic, and metabolomic approach to investigate the molecular mechanisms underlying the plant's resistance to , of which little is known. We found that infection stimulated the outbreak of reactive oxygen species (ROS) in cherry.

Formation Tracking Control for Heterogeneous Multiagent Systems With Multiple Nonautonomous Leaders via Dynamic Event-Triggered Mechanisms.

This article considers the time-varying formation (TVF) tracking issue of heterogeneous multiagent systems (HMASs) with the dynamic event-triggered control. The HMASs contain heterogeneous multiple leaders, all of which have the input signals to generate flexible reference, and only the output information can be measured. All leaders do not have access to the same followers, that is, the well-informed follower assumption is removed in this article.

Adaptive Time-Varying Formation Tracking Control for Multiagent Systems With Nonzero Leader Input by Intermittent Communications.

The time-varying formation (TVF) tracking problem is studied for linear multiagent systems (MASs), where followers reach a preset TVF when tracking the leader's state. Followers are divided into the informed ones, which directly receive the leader's information, and uninformed ones. To alleviate communication requirements, trigger mechanisms are designed for the leader and all edges.

Adaptive Bipartite Event-Triggered Time-Varying Output Formation Tracking of Heterogeneous Linear Multi-Agent Systems Under Signed Directed Graph.

This study investigates the adaptive bipartite event-triggered time-varying output formation tracking for heterogeneous linear multi-agent systems (MASs) under signed directed communication topology. Both cooperative communication and antagonistic communication among agents are considered. The fully distributed bipartite compensator based on the novel composite event-triggered transmission mechanism is first put forward to estimate the state of the leader.

Finite-Time Event-Triggered Output Consensus of Heterogeneous Fractional-Order Multiagent Systems With Intermittent Communication.

The finite-time output consensus (FTOC) issue of heterogeneous fractional-order multiagent systems (HFO-MASs) is investigated in this article. First, a new principle of finite-time convergence for absolutely continuous functions is developed if a fractional derivative inequality is satisfied. Next, in order to remove the assumption that the leader's system matrix is known to all agents in previous studies, a distributed adaptive finite-time observer is designed, which can estimate not only the leader's state but also the leader's system matrix.

The Bipartite Edge-Based Event-Triggered Output Tracking of Heterogeneous Linear Multiagent Systems.

This article focuses on the bipartite output tracking control for heterogeneous linear multiagent systems under the asynchronous edge-based event-triggered transmission mechanism. First, the distributed bipartite edge-based event-triggered compensator is established to estimate the state of the exosystem. The estimated state of the compensator is the same as the state of the exosystem in modulus and opposite in sign because of the existence of antagonistic communications.

Observer-Based Output Feedback Event-Triggered Adaptive Control for Linear Multiagent Systems Under Switching Topologies.

The consensus problem of general linear multiagent systems (MASs) is studied under switching topologies by using observer-based event-triggered control method in this article. On the basis of the output information of agents, two kinds of novel event-triggered adaptive control schemes are designed to achieve the leaderless and leader-follower consensus problems, which do not need to utilize the global information of the communication networks. Finally, two simulation examples are introduced to show that the consensus error converges to zero and Zeno behavior is eliminated in MASs.

Distributed Bipartite Adaptive Event-Triggered Fault-Tolerant Consensus Tracking for Linear Multiagent Systems Under Actuator Faults.

This article considers the distributed bipartite adaptive event-triggered fault-tolerant consensus tracking issue for linear multiagent systems in the presence of actuator faults based on the output feedback control protocol. Both time-varying additive and multiplicative actuator faults are taken into account in the meantime. And the upper/lower bounds of actuator faults are not required to be known.

Metabolomics and Transcriptomics Analyses of Two Contrasting Cherry Rootstocks in Response to Drought Stress.

Drought is one of the main factors affecting sweet cherry yields, and cherry rootstocks can provide a range of tree vigor levels to better match sweet cherries with the characteristics of the soil. To investigate the molecular events of the cherry to water deficiency, we performed transcriptomic and metabolomic analyses of CDR-1 (drought-tolerant cherry rootstock (DT)) and × Gisela 5 (drought-susceptible cherry rootstock (DS)), respectively. The results revealed 253 common drought-responsive genes in leaves and roots in DT and 17 in DS; 59 upregulated metabolites were explored in leaves in DT and 19 were explored in DS.

Adaptive Bipartite Fixed-Time Time-Varying Output Formation-Containment Tracking of Heterogeneous Linear Multiagent Systems.

This study investigates the bipartite fixed-time time-varying output formation-containment tracking issue for heterogeneous linear multiagent systems with multiple leaders. Both cooperative communication and antagonistic communication between neighbor agents are taken into account. First, the bipartite fixed-time compensator is put forward to estimate the convex hull of leaders' states.

Leader-Following Consensus for a Class of Nonlinear Multiagent Systems Under Event-Triggered and Edge-Event Triggered Mechanisms.

Considering that there are many systems with limited network bandwidth in practice, this article studies the leader-following consensus problem for a class of nonlinear multiagent systems (MASs). The purpose of this article is to reduce unnecessary information transmission between any pair of adjacent agents including the leader in the MASs through intermittent communication. The novel event-triggered and asynchronous edge-event triggered mechanisms are designed for the leader and all edges, respectively.

Anticardiolipin Positivity Is Highly Associated With Intrauterine Growth Restriction in Women With Antiphospholipid Syndrome.

The purpose of our study was to evaluate pregnancy outcomes of women with antiphospholipid antibodies (aPL) positivity and assess risk factors associated with adverse pregnancy outcomes. Pregnant women with aPL positivity were enrolled prospectively in China from January 2017 to March 2020. Treatment of low-dose aspirin and low molecular weight heparin were given.

Resistance analysis of cherry rootstock 'CDR-1' (Prunus mahaleb) to crown gall disease.

Background: Crown gall disease, caused by the pathogenic bacterium Agrobacterium tumefaciens, is responsible for extensive economic losses in orchards. Cherry rootstock 'CDR-1' (Prunus mahaleb) shows high resistance but the mechanism remains unclear. Here, we examined the morphology of pathogen-infected root neck surface, determined the activity of 10 defense-related enzymes and the content of salicylic acid (SA) and jasmonic acid (JA), and also applied transcriptome analysis, transient expression and transgenic verification to explore the crown gall resistance genes in 'CDR-1' plants.

Adaptive Bipartite Event-Triggered Output Consensus of Heterogeneous Linear Multiagent Systems Under Fixed and Switching Topologies.

This article addresses the adaptive bipartite event-triggered output consensus issue for heterogeneous linear multiagent systems. We consider both cooperative interaction and antagonistic interaction between neighbor agents in both fixed and switching topologies. An adaptive bipartite compensator consisting of time-varying coupling weights and dynamic event-triggered mechanism is first proposed to estimate the leader's state in a fully distributed manner.

Distributed bipartite leader-following consensus of linear multi-agent systems with input time delay based on event-triggered transmission mechanism.

This study focuses on the distributed bipartite consensus tracking for linear multi-agent systems with input time delay based upon event-triggered transmission mechanism. Both cooperative interaction and antagonistic interaction between neighbor agents are considered. A novel distributed bipartite control technique with event-triggered mechanism is raised to address this consensus issue.

Distributed cooperative output regulation of heterogeneous linear multi-agent systems based on event- and self-triggered control with undirected topology.

In this note, the cooperative output regulation problem is considered for a kind of heterogeneous linear multi-agent systems under undirected communication topological graphs. Our approach consists of two techniques, the design of distributed dynamic observer for exosystem under the event-triggered and self-triggered control mechanisms. Firstly, a distributed event-triggered control protocol is designed, which proves that multi-agent system can achieve stability and Zeno behavior is excluded under this protocol.

Optimizing Suitable Antibiotics for Bacterium Control in Micropropagation of Cherry Rootstock Using a Modified Leaf Disk Diffusion Method and E Test.

Bacterial contamination is a major and constant threat to the establishment and subculture of in vitro plant culture. In this study, we used a slightly modified qualitative disk diffusion method to screen optimal antibiotics to control the growth of bacterial contaminants isolated from explants of cherry rootstock 'Gisela 6'. Bacterial susceptibility to eight different antibiotics was tested.

Dynamic output feedback-based fault-tolerant control design for T-S fuzzy systems with model uncertainties.

This paper is concerned with fault-tolerant control (FTC) for a class of nonlinear discrete-time systems. A dynamic output feedback controller is designed for Takagi-Sugeno (T-S) fuzzy systems with state and input parameter uncertainties, exogenous disturbances, measurement noise and multiple intermittent faults. Compared with the existing research results, the proposed controller has a wider application range.

Molecular identification and genetic analysis of cherry cultivars using capillary electrophoresis with fluorescence-labeled SSR markers.

Molecular identification and genetic analysis of cherry are necessary for solving the problem of synonyms and homonyms that occur in cherry production. In this study, capillary electrophoresis with fluorescent-labeled simple sequence repeat (SSR) primers was used to identify 63 cherry cultivars (varieties and rootstocks) planted in Shaanxi province, China. A total of 146 alleles were amplified by 10 SSR primer pairs, ranging from 10 to 20 per locus (mean: 14); among the SSR primer pairs, genotype number ranged from 12 to 26 (mean: 18).

[Studies on apoptosis and caspase-8 and caspase-9 expressions of bone marrow cells in chronic mountain sickness].

Objective: To observe the expressions of caspase-8 and caspase-9 mRNA, and explore the changes of apoptosis of bone marrow hematopoietic cells in patients with chronic mountain sickness (CMS).

Methods: Of 18 CMS patients and 16 controls were enrolled in this study. The apoptotic index (AI) of bone marrow mononuclear cells (BMMNC) was measured by TUNEL technique, the levels of caspase-8 and caspase-9 mRNA in BMMNC of CMS patients and controls were determined by RT-PCR.