Reduced local functional connectivity correlates with atypical performances in children with autism spectrum disorder.

To characterize local functional connectivity (FC) differences in children with autism spectrum disorder (ASD) compared to typically developed (TD) children, and to analyze the correlation between local FC and the atypical behavior in autistic children. Thirty children with ASD and 25 TD children were recruited. Participants underwent rs-fMRI scans, and regional homogeneity (ReHo) of specific brain regions was measured.

Red blood cells-derived components as biomimetic functional materials: Matching versatile delivery strategies based on structure and function.

Red blood cells (RBCs), often referred to as "intelligent delivery systems", can serve as biological or hybrid drug carriers due to their inherent advantages and characteristics. This innovative approach has the potential to enhance biocompatibility, pharmacokinetics, and provide targeting properties for drugs. By leveraging the unique structure and contents of RBCs, drug-loading pathways can be meticulously designed to align with these distinctive features.

The value of ultrasound indicators in early pregnancy for predicting selective intrauterine growth restriction and twin-twin transfusion syndrome: a case‒control study.

Background: Selective intrauterine foetal growth restriction (sIUGR) and twin-twin transfusion syndrome (TTTS) are common complications in Monochorionic diamniotic (MCDA) twin pregnancies. Timely and accurate diagnosis and intervention are essential to improve perinatal outcomes. The purpose of this study was to determine the value of differences in crown - rump lengths (CRL) and nuchal translucency (NT) and evaluate the significance of differences in embryo length in predicting the occurrence of sIUGR and TTTS.

Multivariate predictive model of the therapeutic effects of metoprolol in paediatric vasovagal syncope: a multi-centre study.

Background: Metoprolol therapy for paediatric vasovagal syncope (VVS) has yielded inconsistent results, necessitating predictive markers. We aimed to develop and validate models to identify paediatric VVS patients likely to benefit from metoprolol.

Methods: 478 metoprolol-treated paediatric patients with VVS were enrolled from three syncope units and divided into retrospective training (March 2017-March 2023, n = 323) and prospective validation cohorts (April 2023-March 2024, n = 155).

Enhancing gamma-ray detection: Processing grooved microstructures on LYSO crystal with femtosecond laser.

Background: Gamma-ray detection plays a crucial role in the fields of biomedicine, space exploration, national defense, and security. High-precision gamma photon detection relies on scintillation crystals, which attenuate gamma rays through mechanisms such as photoelectric effect and Compton scattering. These interactions generate light signals within the scintillation crystal, which are subsequently converted into electronic signals using photodetectors, enabling accurate readout, and analysis.

Integrative analysis of anoikis-related prognostic signature to evaluate the immune landscape and predict therapeutic response in stomach adenocarcinoma.

Stomach adenocarcinoma (STAD) is the most prevalent gastrointestinal malignancy and seriously threatens the life of the global population. Anoikis, a process of programmed cell death that occurs when cells detach from the extracellular matrix, is closely associated with tumor invasion and metastasis. In this study, we used the TCGA-STAD database to identify the expression patterns and prognostic relevance of anoikis-related genes (ARGs) in STAD.

Exosome-derived Uc.339 as a potential biomarker for bone metastasis from pulmonary adenocarcinoma.

This study explored the role of Uc.339, which is a highly expressed genomic sequence in tumor cell-derived exosomes, in mediating bone metastasis from lung adenocarcinoma. By integrating clinical samples, in vitro experiments, and in vivo murine models, we elucidated the molecular mechanisms underlying this process.

Thin Layer Chromatography Goes Ultrasmall to Assay Sphingosine Kinase Activation in Single Primary Leukemic Cells.

Cell-to-cell heterogeneity in lipid signaling underlies variations in response and recurrence for many cancers, including leukemias. A highly parallel, miniaturized thin-layer chromatographic platform capable of assaying single cells was developed. Ultrasmall volumes (50 pL) of standard fluorescent lipids were separated with excellent repeatability, reproducibility, and limits of detection.

Evaluation of Optimized Lumbar Oblique X-Ray Angles with Positioning Assistance for Enhanced Imaging Quality: A Pilot Study in an Asian Cohort.

Pars fractures are a common cause of lower back pain, especially among young individuals. Although computed tomography (CT) and magnetic resonance imaging (MRI) scanning are commonly used in developed regions, traditional radiography remains the main diagnostic method in many developing countries. This study assessed whether the standard radiographic angles suggested in textbooks are optimal for an Asian population since Asian groups have lower lumbar lordosis.

The Dynamic Changes of COL11A1 Expression During the Carcinogenesis and Development of Breast Cancer and as a Candidate Diagnostic and Prognostic Marker.

Collagen type XI alpha 1 (COL11A1), a critical member of the collagen superfamily, is essential for tissue structure and integrity. This study aimed to validate previously identified variations in COL11A1 expression during breast cancer carcinogenesis and progression, as well as elucidate their clinical implications. COL11A1 mRNA expression levels were assessed using real-time reverse transcription-PCR (RT-PCR) in 30 pairs of normal breast tissue and primary breast cancer, 30 pairs of primary breast cancer and lymph node metastases, 30 benign tumors, and 107 primary breast cancers.

Bioactive materials from berberine-treated human bone marrow mesenchymal stem cells accelerate tooth extraction socket healing through the jaw vascular unit.

Delayed tooth extraction socket (TES) healing can cause failure of subsequent oral implantation and increase socioeconomic burden on patients. Excessive amounts of M1 macrophages, apoptotic neutrophils (ANs), and neutrophil extracellular traps (NETs) impair alveolar bone regeneration during TES healing. In the present study, we first discovered that conditioned medium (CM) collected from berberine-treated human bone marrow mesenchymal stem cells (BBR-HB-CM) accelerated TES healing.

Association of Dietary Intake of Vitamin A With Adolescent Hypertension: A Cross-Sectional Study Based on NHANES 1999-2018.

We aimed to investigate the association between dietary intake of vitamin A and risk of hypertension during adolescence. We interrogated the National Health and Nutrition Examination Survey (NHANES) database, from which individual-level data on dietary intake of vitamin A were garnered from 13,909 adolescents (aged 10-19 years) participating in the 1999-2018 study cycle. After dividing vitamin A intake into four quartiles, we leveraged weighted multivariate logistic regression to investigate the association of vitamin A intake with hypertension by each quartile, with the restricted cubic spline (RCS) curve plotted to assess the nonlinearity of association.

Improving Diagnostic Accuracy in Acute Pulmonary Embolism: Insights from Spectral Dual-energy CT.

Purpose: This study aims to evaluate the clinical efficacy of spectral dual-energy detector computed tomography (SDCT) and its associated parameters in diagnosing acute pulmonary embolism (APE).

Methods: Retrospective analysis of imaging data from 86 APE-diagnosed patients using SDCT was conducted. Virtual monoenergetic images (VMIs) at 40, 70, and 100 KeV, Iodine concentration (IC) maps, Electron Cloud Density Map (ECDM), Effective atomic number (Z-eff) maps, and Hounsfield unit attenuation plots (VMI slope) were reconstructed from pulmonary artery phase CT images.

Fibroblasts modulate epithelial cell behavior within the proliferative niche and differentiated cell zone within a human colonic crypt model.

Colonic epithelium is situated above a layer of fibroblasts that provide supportive factors for stem cells at the crypt base and promote differentiation of cells in the upper crypt and luminal surface. To study the fibroblast-epithelial cell interactions, an crypt model was formed on a shaped collagen scaffold with primary epithelial cells growing above a layer of primary colonic fibroblasts. The crypts possessed a basal stem cell niche populated with proliferative cells and a differentiated, nondividing cell zone at the luminal crypt end.

A preclinical study of allogeneic CD19 chimeric antigen receptor double-negative T cells as an off-the-shelf immunotherapy drug against B-cell malignancies.

Objectives: To evaluate the manufacturability, efficacy and safety of allogeneic CD19 chimeric antigen receptor double-negative T cells (CD19-CAR-DNTs) as an off-the-shelf therapeutic cell product.

Methods: A membrane proteome array was used to assess the off-target binding of CD19-CAR. DNTs derived from healthy donors were transduced with lentiviral vectors encoding the CD19-CAR.

Positive regulation of a LuxR family protein, MilO, in mildiomycin biosynthesis.

Mildiomycin is a representative peptidyl nucleoside antibiotic and was first isolated from , which has been used as an important biological agent to control powdery mildew in plants. Despite its importance, the biosynthetic pathways and regulatory mechanisms remain to be fully elucidated. In this study, we identified MilO as a positive pathway-specific regulator of mildiomycin biosynthesis in the heterologous host .

Essential thrombocytosis transformed AML with TP53 mutations and its clinical implications.

Essential thrombocytosis (ET) is a chronic myeloproliferative neoplasm. There is a rare possibility of its transformation from ET into acute myeloid leukemia (AML). While the TP53 mutation is a well-known risk factor for AML, limited research exists regarding ET patients who develop AML with TP53 mutations.

Engineering Dual Active Sites and Defect Structure in Nanozymes to Reprogram Jawbone Microenvironment for Osteoradionecrosis Therapy.

Four to eight percent of patients with head and neck cancer will develop osteoradionecrosis of the jaw (ORNJ) after radiotherapy. Various radiation-induced tissue injuries are associated with reactive oxygen and nitrogen species (RONS) overproduction. Herein, Fe doping is used in VO (Fe-VO) nanozymes with multienzyme activities for ORNJ treatment via RONS scavenging.

A novel prediction model for the prognosis of non-small cell lung cancer with clinical routine laboratory indicators: a machine learning approach.

Background: Lung cancer is characterized by high morbidity and mortality due to the lack of practical early diagnostic and prognostic tools. The present study uses machine learning algorithms to construct a clinical predictive model for non-small cell lung cancer (NSCLC) patients.

Methods: Laboratory indices of the NSCLC patients at their initial visit were collected for quality control and exploratory analysis.

Performance Investigations of Two Channel Readout Configurations on the Cross-Strip Cadmium Zinc Telluride Detector.

In a detector system where the number of channels exceeds the number of channels available on an application-specific integrated circuit (ASIC), there is a need to configure channels among multiple ASICs to achieve the lowest electronic noise and highest count rate. In this work, two board configurations were designed to experimentally assess which one provides the more favorable performance. In the half-half configuration, contiguous channels from one edge to the center of CZT detector are read by one ASIC, and the other half are read by the other ASIC.

Cerebral biomimetic nano-drug delivery systems: A frontier strategy for immunotherapy.

Brain diseases are a significant threat to human health, especially in the elderly, and this problem is growing as the aging population increases. Efficient brain-targeted drug delivery has been the greatest challenge in treating brain disorders due to the unique immune environment of the brain, including the blood-brain barrier (BBB). Recently, cerebral biomimetic nano-drug delivery systems (CBNDSs) have provided a promising strategy for brain targeting by mimicking natural biological materials.

A UPLC-MS/MS coupled with GC-MS method for quantification of twenty-one chemical ingredients from Suxiao Jiuxin pill in multiple tissue of rat and its application to tissue distribution study.

Suxiao Jiuxin pill (SJP) was a commonly-used traditional Chinese medicine for treating cardiovascular diseases. It was composed of the rhizome of Ligusticum chuanxiong Hort. and Borneolum Syntheticum.

Bmi-1 Epigenetically Orchestrates Osteogenic and Adipogenic Differentiation of Bone Marrow Mesenchymal Stem Cells to Delay Bone Aging.

With the increase in the aging population, senile osteoporosis (SOP) has become a major global public health concern. Here, it is found that Prx1 and Bmi-1 co-localized in trabecular bone, bone marrow cavity, endosteum, and periosteum. Prx1-driven Bmi-1 knockout in bone-marrow mesenchymal stem cells (BMSCs) reduced bone mass and increased bone marrow adiposity by inhibiting osteoblastic bone formation, promoting osteoclastic bone resorption, downregulating the proliferation and osteogenic differentiation of BMSCs, and upregulating the adipogenic differentiation of BMSCs.