Ultralow Potential Cathodic Electrochemiluminescence Aptasensor for Detection of Kanamycin Using Copper Nanoribbons as Coreaction Accelerator.

An ultralow cathodic potential electrochemiluminescence (ECL) aptasensor was designed, employing DNA nanoribbon template self-assembly copper nanoclusters (DNR-CuNCs) as a novel coreaction accelerator within the luminol-HO system for the sensitive detection of kanamycin (KANA). Mechanistic investigations revealed that the DNR-CuNCs preferred to generate highly active hydroxyl radicals by facilitating the reduction of the coreactant HO under neutral pH conditions, consequently enhancing cathodic luminescence. By the strong π-π stacking effect of KANA aptamer and graphene as a signal modulation switch, DNR-CuNCs were displaced from the electrode surface due to the affinity of KANA and its aptamer, resulting in the inhibition of the luminol-HO system and a decrease in the ECL signal.

Transcriptome-Based Identification of the SaR2R3-MYB Gene Family in and Function Analysis of SaR2R3-MYB15 in Salt Stress Tolerance.

As one of the most prominent gene families, R2R3-MYB transcription factors significantly regulate biochemical and physiological processes under salt stress. However, in , a perennial herb known for its exceptional saline alkali resistance, the comprehensive identification and characterization of SaR2R3-MYB genes and their potential functions in response to salt stress have yet to be determined. We investigated the expression profiles and biological functions of SaR2R3-MYB transcription factors in response to salt stress, utilizing a transcriptome-wide mining method.

Sensitive sensing platform based on Co, Mo doped electrospun nanofibers for simultaneous electrochemical detection of dopamine and uric acid.

Abnormal levels of dopamine (DA) and uric acid (UA) in the human body are valuable indicators for monitoring human health, as they are associated with certain diseases. Therefore, it is crucial to develop sensitive and simultaneous analytical techniques for DA and UA in diagnosing the related diseases. Herein, the Co- and Mo- doped carbon nanofibers (Co, Mo@CNFs) electrochemical biosensor was developed successfully for the sensitive and accurate simultaneous detection of DA and UA.

Self-assembly of Copper Nanoclusters Using DNA Nanoribbon Templates for Sensitive Electrochemical Detection of HO in Live Cells.

The excessive secretion of HO within cells is closely associated with cellular dysfunction. Therefore, high sensitivity in situ detection of HO released from living cells was valuable in clinical diagnosis. In the present work, a novel electrochemical cells sensing platform by synthesizing copper nanoclusters (CuNCs) at room temperature based on DNA nanoribbon (DNR) as a template (DNR-CuNCs).

Anaerobic granular sludge performance in an expanded granular sludge bed reactor treating calcium-rich wastewater by adjusting CaCO crystallization: Effect of upflow velocity and Ca concentration.

The role of upflow velocity and Ca concentration in controlling the type and rate of CaCO crystallization and their impacts on the anaerobic granular sludge (AnGS) formation and performance in an expanded granular sludge bed (EGSB) reactor were studied. The results showed that an improved upflow velocity could promote metastable CaCO crystals and achieve the optimized portion of vaterite with a value of 84 % at 10 m/h with a small amount of aragonite, thus limiting the scaling in the reactor. The removal efficiency of Ca was to some extent positively correlated to the influent Ca concentration, but declined when Ca exceeded a specific threshold.

Intracellular pools of FcalphaR (CD89) in human neutrophils are localized in tertiary granules and secretory vesicles, and two FcalphaR isoforms are found in tertiary granules.

The human FcalphaRIota (CD89) is expressed on cells of myeloid lineage and plays an important role in host defense. Neutrophils make up the majority of FcalphaRIota-positive cells. Previous reports suggested that FcalphaR was stored in neutrophil intracellular pools, and it could be mobilized quickly once neutrophils were activated.