Publications by authors named "Yuko Onda"
Article Synopsis
- Sapovirus (SaV) infections are on the rise globally, with a notable increase in Japan from 2010 to 2022, mainly due to the GI.1 genotype.
- The study analyzed 15 SaV GI.1 outbreak strains using next-generation sequencing and found distinct clustering in lineages and specific amino acid substitutions in their genomes.
- Key mutations associated with the pathogenicity and spread of SaV were identified in strains responsible for the largest outbreak in Japan (2021-2022), enriching the understanding of SaV's genetic makeup.
The FilmArray® Gastrointestinal (GI) Panel is a modern, sensitive, and comprehensive stool testing technique for identifying common gastrointestinal pathogens, including viruses, bacteria, and parasites. Its increasing demand is due to ease of operation and automation. Pathogens, particularly viruses, undergo constant genetic evolution.
View Article and Find Full Text PDFHuman adenovirus (HAdV) is one of the causative viruses of acute gastroenteritis (AGE) in children worldwide. Species F is known to be enteric adenovirus (genotypes 40 and 41) detected in stool samples. In Japan, we conducted an epidemiological study and molecular characterization of HAdV before and after the COVID-19 pandemic from 2017 to 2023.
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- Wastewater-based epidemiology (WBE) proved crucial during the COVID-19 pandemic as patients avoided clinics, allowing for ongoing tracking of enteric viruses in communities.
- A study was conducted analyzing raw sewage from Japan's Tohoku and Kansai regions from 2019 to 2022, focusing on major acute gastroenteritis (AGE) viruses using RT-PCR and sequence analysis.
- Findings revealed high prevalence of rotavirus A and astrovirus, with no decline in enteric viruses during the pandemic, while some strains, like norovirus GII.2, emerged and spread across the regions.
Article Synopsis
- * Raw sewage samples from Japan (2018-2022) showed a significant rise (10-20%) in AGE viruses such as rotavirus and norovirus during the pandemic, while some others saw small decreases.
- * The research highlighted the emergence and increase of multiple viral strains during this period, emphasizing the need to monitor sewage water for a better understanding of ongoing viral activity when clinical data may be lacking.
Although metabolome research is a rapidly expanding field in the postgenomic era, no single method exists for complete analysis of all the constituents of a metabolome. In this study, we developed a metabolome analysis method using a combination of capillary electrochromatography and electrospray ionization-mass spectrometry. The capillary electrochromatography column was prepared by surface modification of silica compounds (tetraethoxysilane and octyltriethoxysilane) in a fused-silica capillary column.
View Article and Find Full Text PDFA new capillary electrochromatography (CEC) column for the simultaneous analysis of cationic, neutral, and anionic compounds using CEC-ESI-MS is described. Three different silica monolith columns were prepared by changing the poly(ethylene glycol) (PEG) contents for comparison of the separation property of these columns. Different separation programs were used for the simultaneous separation of different charged compounds under the same conditions.
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