Publications by authors named "Yukio Nagaosa"

Formate oxidase of Aspergillus oryzae RIB40 contains an 8-replaced FAD with molecular mass of 799 as cofactor. The ¹H-NMR spectrum of the cofactor fraction obtained from the enzyme indicated that the 8-replaced FAD in the fraction was 8-formyl-FAD, present in open form and hemiacetal form. The oxidation-reduction potentials of the open and hemiacetal forms were estimated by cyclic voltammetry to be -47 and -177 mV vs.

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The use of 1-butyl-3-methylimidazolium hexafluorophosphate (BMIM x PF(6)) as a solvent for the anodic stripping analysis of Pb(II) and Cd(II) after extraction of the iodide complexes has been investigated. This method is based on the extraction of the metal iodide complexes into BMIM x PF(6), followed by differential-pulse anodic stripping voltammetry with an in-situ plated bismuth film electrode onto an edge-plane type of pyrolytic graphite substrate. When tetra-n-propylammonium iodide at a concentration of 0.

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A very sensitive and simple method is presented for the determination of Se(IV) by Osteryang square-wave cathodic stripping voltammery (OSWCSV). The method is based on the reduction of Se(IV) with Bi(III) onto an edge-plane type of pyrolytic graphite substrate, followed by a cathodic potential scan. OSWCSV studies indicate that the reduced selenium produced a distinct catalytic hydrogen wave at -1150 mV vs.

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A highly sensitive method has been developed for the determination of trace As(III) by a square wave cathodic stripping voltammetry employing in situ plated bismuth-film on edge-plane graphite substrate as working electrode. The presence of As(III) enhanced a cathodic peak corresponding to the catalytic hydrogen wave due to Se(IV) at about -1150 mV. Linear calibration curves for As(III) determination were obtained over the concentration ranges of 0.

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A sensitive and selective method has been developed for the simultaneous determination of cadmium, zinc, nickel and cobalt. The method is based on the chelation of metal ions with 2-(8-quinolylazo)-4,5-diphenylimidazole (QAI) and the subsequent reversed-phase (RP) high-performance liquid chromatographic separation and spectrophotometric detection of the metal chelates. The chelates were separated on an RP column with acetonitrile-water containing ethylenediamine tetraacetic acid and sodium acetate (pH 7.

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An aldehyde oxidase, which oxidizes various aliphatic and aromatic aldehydes using O(2) as an electron acceptor, was purified from the cell-free extracts of Pseudomonas sp. KY 4690, a soil isolate, to an electrophoretically homogeneous state. The purified enzyme had a molecular mass of 132 kDa and consisted of three non-identical subunits with molecular masses of 88, 39, and 18 kDa.

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