Nitric oxide represses the proliferation of Caco-2 cells by inducing S-G/M cell cycle arrest.

There is conflicting data regarding the ability of nitric oxide (NO) to promote or inhibit colorectal cancer cell proliferation. Furthermore, NO reacts rapidly with endogenous superoxide at a diffusion-controlled rate to give peroxynitrite (ONOO), a strong oxidant and nitrating agent. The aim of this study was to assess the effects of exogenous NO and ONOO on the proliferation of the colorectal cancer cell line Caco-2.

Hydrogen sulfide donor GYY4137 suppresses proliferation of human colorectal cancer Caco-2 cells by inducing both cell cycle arrest and cell death.

Conflicting data regarding the ability of hydrogen sulfide (HS), which reaches high levels in the large intestine owing to biosynthesis in the intestinal cells and intestinal bacteria, to promote or inhibit colorectal cancer cell proliferation have been reported recently. In the present study, the effect of HS on the proliferation of the human colorectal cancer cell line Caco-2 was examined by using the HS donor GYY4137. At concentrations of 0.