Purification, characterization, and application of a high activity 3-ketosteroid-Δ-dehydrogenase from Mycobacterium neoaurum DSM 1381.

Δ-Dehydrogenation is one of the most important reactions for steroid drug modification. Numerous 3-ketosteroid-Δ-dehydrogenases (KstDs) catalyzing this reaction were observed in various organisms. However, only a few have been characterized and used for substrate conversion.

Non-capsulated mutants of a chemical-producing Klebsiella pneumoniae strain.

Objectives: To investigate the outcomes of capsule lost on cell transformation efficiency and chemicals (1,3-propanediol, 2,3-butanediol, and 2-ketogluconic acid) production by Klebsiella pneumoniae.

Results: The cps gene cluster showed low sequence homology with pathogenic strains. The wza is a highly conserved gene in the cps cluster that encodes an outer membrane protein.