Generation of rat-derived lung epithelial cells in Fgfr2b-deficient mice retains species-specific development.

Regenerative medicine is a tool to compensate for the shortage of lungs for transplantation, but it remains difficult to construct a lung in vitro due to the complex three-dimensional structures and multiple cell types required. A blastocyst complementation method using interspecies chimeric animals has been attracting attention as a way to create complex organs in animals, although successful lung formation using interspecies chimeric animals has not yet been achieved. Here, we applied a reverse-blastocyst complementation method to clarify the conditions required to form lungs in an Fgfr2b-deficient mouse model.

Color changes in bulk-fill resin composites as a result of visible light-curing.

We evaluated twelve commercially available bulk-fill resin composites to determine the colorimetric changes that occurred as a result of the photo-polymerization reaction. Samples of the resin composites were prepared as disks 8 mm in diameter and 3 mm in thickness. A colorimeter was used to measure the color of samples on a black background before and after the polymerization processed had been initiated.

Use of new scratch test and tensile test for evaluation of bond strength of selfadhesive flowable resin composite for repair of artificial tooth erosion.

The purpose of this study was to use a new scratch test and tensile test to evaluate the bond strength between artificial erosive enamel or dentin and self-adhesive resin composites as a coating material. Coronal enamel or dentin surface was exposed to an erosive cycle (artificial saliva [AS], pH:7.0 for 6.

Characterization of the Human Transcription Elongation Factor Rtf1: Evidence for Nonoverlapping Functions of Rtf1 and the Paf1 Complex.

Restores TBP function 1 (Rtf1) is generally considered to be a subunit of the Paf1 complex (PAF1C), a multifunctional protein complex involved in histone modification and transcriptional or posttranscriptional regulation. Rtf1, however, is not stably associated with the PAF1C in most species except Saccharomyces cerevisiae, and its biochemical functions are not well understood. Here, we show that human Rtf1 is a transcription elongation factor that may function independently of the PAF1C.

A novel DDS strategy, "dual-targeting", and its application for antineovascular therapy.

Dual-targeting liposomes modified with Ala-Pro-Arg-Pro-Gly (APRPG) and Gly-Asn-Gly-Arg-Gly (GNGRG) peptides were developed. They remarkably associated to growing human umbilical vein endothelial cells (HUVECs) compared with single-targeting liposomes modified with APRPG or GNGRG. Doxorubicin (DOX) encapsulated in the dual-targeting liposomes significantly suppressed the growth of HUVECs compared with that in single-targeting liposomes.