Publications by authors named "Yuki Kawai-Harada"
Article Synopsis
- RecombiCraft is a new and cost-effective method for rapidly creating DNA libraries in E. coli by using SLiCE and liquid culture amplification.
- The approach utilizes E. coli's natural abilities to recombine DNA, streamlining the process by eliminating the need for multiple enzymes and minimizing sequence biases.
- Overall, this technique can generate DNA libraries in approximately 12 hours, making it a valuable tool for genetic research and biotechnology.
Extracellular vesicles (EVs), detectable in all bodily fluids, mediate intercellular communication by transporting molecules between cells. The capacity of EVs to transport molecules between distant organs has drawn interest for clinical applications in diagnostics and therapeutics. Although EVs hold potential for nucleic-acid-based and other molecular therapeutics, the lack of standardized technologies, including isolation, characterization, and storage, leaves many challenges for clinical applications, potentially resulting in misinterpretation of crucial findings.
View Article and Find Full Text PDFExtracellular vesicles (EVs) are naturally released, cell-derived vesicles that mediate intracellular communication, in part, by transferring genetic information and, thus, have the potential to be modified for use as a therapeutic gene or drug delivery vehicle. Advances in EV engineering suggest that directed delivery can be accomplished via surface alterations. Here we assess enriched delivery of engineered EVs displaying an organ targeting peptide specific to the pancreas.
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