Publications by authors named "Yuki Horisawa-Takada"
Article Synopsis
- The study focuses on how gene expression and chromatin changes occur during spermatogenesis, especially during meiotic prophase.
- Researchers discovered a protein complex made up of ZFP541 and KCTD19 that is crucial for regulating transcription during this phase in male mice.
- ZFP541 is shown to bind to and suppress various genes linked to transcription and chromatin modifications, which helps in ensuring the proper progression and completion of meiotic prophase.
Article Synopsis
- - Meiosis is a complex cell division process, and researchers identified the gene Kctd19 as crucial for this process in male mice using CRISPR/Cas9 to screen genes involved in reproduction.
- - Kctd19 deficiency didn't hinder early meiosis stages like synapsis but did cause spermatocytes to undergo apoptosis during the transition from metaphase to anaphase.
- - The study also found that KCTD19 interacts with proteins ZFP541 and HDAC1, with both KCTD19 and ZFP541 being essential for normal meiosis, specifically in preventing DNA damage in spermatocytes.
During meiotic prophase, sister chromatids are organized into axial element (AE), which underlies the structural framework for the meiotic events such as meiotic recombination and homolog synapsis. HORMA domain-containing proteins (HORMADs) localize along AE and play critical roles in the regulation of those meiotic events. Organization of AE is attributed to two groups of proteins: meiotic cohesins REC8 and RAD21L; and AE components SYCP2 and SYCP3.
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