The study evaluated the impact of different fixation durations in 96% alcohol on protein preservation for immunohistochemistry compared to the standard 10% neutral buffered formalin (NBF).
Researchers used 25 carcinoma specimens, fixing them in both solutions for various timeframes (1, 6, 24, 48, and 72 hours) and performed cell block preparation to assess E-cadherin and Ki67 expression.
Results showed significant differences in protein expression between 96% alcohol and NBF, concluding that 96% alcohol is not an appropriate fixative for preparing samples for E-cadherin and Ki67 evaluation.