Publications by authors named "Yukari Morita-Fujisawa"
Identification of GM-CSF in Paneth cells using single-cell RT-PCR.
Biochem Biophys Res Commun
December 2003
Paneth cells, granule-containing cells located at the bottom of the intestinal crypts, have a role in innate mucosal immunity. We identified the exclusive expression of granulocyte-macrophage colony-stimulating factor (GM-CSF) in Paneth cells using single-cell reverse transcription-polymerase chain reaction and cDNA array. Cytosolic total RNA was aspirated from single Paneth cells and other villous epithelial cells (non-Paneth cells) of rats using capillary micropipettes.
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- * In experiments, male Wistar rats were treated with dithizone, and messenger RNAs were extracted to create a cDNA library, which was then tested on human intestinal cells (Caco-2) to find growth factors.
- * One significant finding was the rat thioredoxin (TRX) gene, which promoted growth in Caco-2 cells, and its expression was linked to the healing process in the intestines after Paneth cell destruction.