Differences in gaze behavior between driving instructors and older adults during viewing of virtual reality driving videos.

Objective: This study aimed to analyze gaze behavior when viewing real-world driving videos in a virtual reality (VR) environment.

Methods: A total of 22 driving instructors and 36 older drivers, all with regular driver's licenses, participated in this study. Participants watched 360° real-world driving videos using a head-mounted display equipped with eye-tracking functionality (FOVE 0).

Kinetic and Theoretical Studies on Ni /N-Heterocyclic Carbene-Catalyzed Intramolecular Alkene Hydroacylation.

A combined kinetic and theoretical study was conducted in order to clarify the details on the reaction mechanism for Ni /ItBu-catalyzed intramolecular alkene hydroacylation. The results confirm the hypothesis that this intramolecular hydroacylation proceeds through an oxanickelacycle key intermediate.

Nickel(0)-catalyzed intramolecular reductive coupling of alkenes and aldehydes or ketones with hydrosilanes.

A nickel(0)-catalyzed reductive coupling of aldehydes and simple alkenes with hydrosilanes has been developed. A variety of silyl-protected 1-indanol derivatives were prepared in a highly diastereoselective manner (up to >99 : 1 dr) by employing a combination of nickel(0)/N-heterocyclic carbene and triethylsilane. The present system was also applied to a reductive coupling with ketones.

A novel non-canonical Notch signaling regulates expression of synaptic vesicle proteins in excitatory neurons.

Notch signaling plays crucial roles for cellular differentiation during development through γ-secretase-dependent intramembrane proteolysis followed by transcription of target genes. Although recent studies implicate that Notch regulates synaptic plasticity or cognitive performance, the molecular mechanism how Notch works in mature neurons remains uncertain. Here we demonstrate that a novel Notch signaling is involved in expression of synaptic proteins in postmitotic neurons.

Comparison of lumbar drainage and external ventricular drainage for clearance of subarachnoid clots after Guglielmi detachable coil embolization for aneurysmal subarachnoid hemorrhage.

Objective: Subarachnoid clots play an important role in development of delayed vasospasm after subarachnoid hemorrhage (SAH). The purpose of this study was to compare clearance of subarachnoid clots using external ventricular drainage (EVD) or lumbar drainage (LD) after Guglielmi detachable coil (GDC) embolization for aneurysmal SAH.

Methods: The subjects were 51 treated with GDC coil embolization for aneurysmal Fisher group 3 SAH within 72 h of ictus.

Activity-dependent proteolytic cleavage of neuroligin-1.

Neuroligin (NLG), a postsynaptic adhesion molecule, is involved in the formation of synapses by binding to a cognate presynaptic ligand, neurexin. Here we report that neuroligin-1 (NLG1) undergoes ectodomain shedding at the juxtamembrane stalk region to generate a secreted form of NLG1 and a membrane-tethered C-terminal fragment (CTF) in adult rat brains in vivo as well as in neuronal cultures. Pharmacological and genetic studies identified ADAM10 as the major protease responsible for NLG1 shedding, the latter being augmented by synaptic NMDA receptor activation or interaction with soluble neurexin ligands.

Selective and regulated gene expression in murine Purkinje cells by in utero electroporation.

Cerebellar Purkinje cells, which convey the only output from the cerebellar cortex, play an essential role in cerebellar functions, such as motor coordination and motor learning. To understand how Purkinje cells develop and function in the mature cerebellum, an efficient method for molecularly perturbing them is needed. Here we demonstrate that Purkinje cell progenitors at embryonic day (E)11.

Alterations of glucose metabolism in Escherichia coli mutants defective in respiratory-chain enzymes.

The effects of reduced efficiency of proton-motive force (pmf) generation on glucose metabolism were investigated in Escherichia coli respiratory-chain mutants. The respiratory chain of E. coli consists of two NADH dehydrogenases and three terminal oxidases, all with different abilities to generate a pmf.