Publications by authors named "Yuka Tokutake"

Article Synopsis
  • The study investigates the levels of malonyl-CoA in the hypothalamus and its relationship with feeding behavior and obesity in Zucker and Zucker diabetic fatty (ZDF) rats.
  • Despite obese ZDF rats having higher leptin, insulin, and glucose levels, their hypothalamic malonyl-CoA levels remain low, similar to that of lean rats.
  • The analysis shows that ZDF rats have larger total CoA pools in their livers compared to Zucker rats, suggesting that obesity may hinder thermoregulation due to lower CoA levels in certain tissues.
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Depression induces anorexia, leading to suppressed feeding behaviors and energy intake. Previously, we revealed that chronic social defeat induced a mild suppression of feeding in rats with elevated levels of hypothalamic malonyl-CoA which regulates feeding. Therefore, we attempted to elucidate the effects of chronic mild food restriction on behavior and on hypothalamic malonyl-CoA.

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The relationship between host factors and cancer cachexia was investigated. A single cell clone (clone 5 tumor) established from colon 26 adenocarcinoma by limiting dilution cell cloning methods was employed to eliminate the inoculation site-dependent differences in the composition of cell clones. Clone 5 tumor did not provoke manifestations of cancer cachexia when inoculated in subcutaneous tissue.

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Article Synopsis
  • LKB1 plays a crucial role in the skeletal muscle by phosphorylating AMPK, which helps regulate fuel oxidation and exercise capacity.
  • In experiments with mice lacking functional LKB1 and AMPK in their skeletal muscles, significant reductions in exercise performance and changes in muscle fiber types were observed, highlighting LKB1's importance.
  • The research concluded that LKB1 is essential for optimal exercise capacity and oxygen uptake in muscles, and it influences fiber type distribution independently of AMPK.
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Pantothenate synthetase (PanC) and pantothenate kinase which function in the canonical coenzyme A (CoA) biosynthetic pathway cannot be found in most archaea. COG1829 and COG1701 intrinsic to archaea were proposed as the candidate proteins for producing 4'-phosphopantothenate instead, and the COG1701 protein from Methanosarcina mazei was assigned as PanC. Meanwhile, the Thermococcus kodakarensis COG1829 and COG1701 proteins were biochemically identified as novel enzymes, i.

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Three coenzyme A (CoA) molecular species, i.e., acetyl-CoA, malonyl-CoA, and nonesterified CoA (CoASH), in 13 types of fasted rat tissue were analyzed.

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Suppression of body weight and eating disorders, such as anorexia, are one of the major symptoms of psychiatric disorders such as depression. However, the mechanisms of weight loss and reduced appetite in depressive patients and in animal models of depression are largely unknown. In this study, we characterized the mechanism of anorexia resulting from depression using socially defeated rats as an animal model of depression.

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Levels of three coenzyme A (CoA) molecular species, i.e., nonesterified CoA (CoASH), acetyl-CoA, and malonyl-CoA, in fasted and fed rat tissues were analyzed by the acyl-CoA cycling method which makes detection possible at the pmol level.

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Acetate is activated to acetyl-CoA by acetyl-CoA synthetase 2 (AceCS2), a mitochondrial enzyme. Here, we report that the activation of acetate by AceCS2 has a specific and unique role in thermogenesis during fasting. In the skeletal muscle of fasted AceCS2(-/-) mice, ATP levels were reduced by 50% compared to AceCS2(+/+) mice.

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The American diet, especially that of adolescents, contains highly palatable foods of high-energy content and large amounts of high-fructose sweeteners. These factors are believed to contribute to the obesity epidemic and insulin resistance. Previous investigations revealed that the central metabolism of glucose suppresses food intake mediated by the hypothalamic AMP-kinase/malonyl-CoA signaling system.

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