Publications by authors named "Yuka Kazo"
Ribosome dimerization is essential for the efficient regrowth of Bacillus subtilis.
Microbiology (Reading)
March 2016
Article Synopsis
- Ribosome dimers, inactive ribosome forms, were found in Bacillus subtilis during the early stationary phase and remained until regrowth started.
- The formation of these dimers was dependent on the hpf gene, which promotes ribosome hibernation, and was expressed throughout the stationary phase.
- While lacking ribosome dimers did not affect the viability of cells during the stationary phase, Δhpf mutant cells showed slower regrowth due to delayed restoration of their translational activity.
The impacts of plasmid carriage on the host cell were comprehensively analysed using the conjugative plasmid pCAR1 in three different Pseudomonas hosts, P. putida KT2440, P. aeruginosa PAO1 and P.
View Article and Find Full Text PDFArticle Synopsis
- - The study focused on the roles of small (p)ppGpp synthetases YjbM and YwaC in Bacillus subtilis by creating mutant strains that had these genes inducibly expressed using IPTG.
- - While the YjbM-expressing strain showed normal growth under induction, the YwaC strain's growth stopped early during exponential growth, leading to a significant drop in intracellular GTP levels and changes in gene expression.
- - Analysis of ribosomal components from the YwaC strain revealed the presence of 70S ribosome dimers—similar to those seen in E. coli—requiring an additional protein, YvyD, for their formation, as the yvyD-disrupted