Characterization of D-galacturonate reductase purified from the psychrophilic yeast species Cryptococcus diffluens.

D-Galacturonic acid reductase was purified from a psychrophilic yeast strain of Cryptococcus diffluens, which was isolated from Satho, a traditional alcohol drink in Thailand. This enzyme, named Cd-GalUAR, assimilates D-galacturonic acid and requires NADPH as a cofactor. Cd-GalUAR is about 45 kDa and stable from pH 6.

Identification and characterization of a psychrophilic yeast strain newly isolated from the fermentative starter (Loog-pang) of a traditional drink in Thailand.

One psychrophilic yeast strain, that grew well in a cold environment such as in a refrigerator, was isolated from the yeast starter (Loog-pang) of a traditional alcohol drink in Thailand. The isolated strain OPU-FC11 was identified as Cryptococcus diffluens by the assay for 26S ribosomal DNA and the test for carbon source assimilation. OPU-FC11 showed a good amount of growth at 4 degrees 0 at which a commonly found yeast like Saccharomyces cerevisiae did not grow, and produced cold-adapted enzymes that showed a relatively high activity at lower temperatures.