Bioinformation study of immune microenvironment characteristics of disulfidptosis-related subtypes in ovarian cancer and prognostic model construction.

Objective: Ovarian cancer significantly impacts women's reproductive health and remains challenging to diagnose and treat. Despite advancements in understanding DNA repair mechanisms and identifying novel therapeutic targets, additional strategies are still needed. Recently, a novel form of cell death called disulfidptosis, which is triggered by glucose deprivation, has been linked to treatment resistance and changes in the tumor microenvironment (TME).

Effects of Pretreatment Methods on Gamma-Aminobutyric Acid Enrichment and Quality Improvement in Highland Barley Beverages.

Gamma-aminobutyric acid (GABA) is an important neurotransmitter that promotes sleep and reduces anxiety, but its natural synthesis in the body is insufficient, necessitating dietary intake. This study utilized a combination of germination, the addition of active barley powder, and fermentation to enhance GABA content in an enzymatically hydrolyzed highland barley beverage. The samples were divided into five groups: highland barley (HB), germinated highland barley (GB), highland barley supplemented with another high-glutamic-acid decarboxylase-active highland barley powder TB13 (BT), germinated barley supplemented with TB13 (GBT), and germinated barley supplemented with TB13 followed by fermentation (GBTF).

PKM2 is a key factor to regulate neurogenesis and cognition by controlling lactate homeostasis.

Adult hippocampal neurogenesis (AHN), the process of generating new neurons from adult neural stem/progenitor cells (NSPCs), is crucial for cognitive functions and is influenced by numerous factors, including metabolic processes. Pyruvate kinase M2 (PKM2), a key rate-limiting enzyme in glycolysis, catalyzes the production of pyruvate, which undergoes either oxidative phosphorylation or anaerobic oxidation. We observed that PKM2 is highly expressed in NSPCs, but its significance remains unclear for AHN and cognition.

Near-infrared downshifting luminescence of CaLaTaO:Nd/Yb/K phosphors and their applications: solar cells and anti-counterfeiting.

A series of Nd/Yb co-doped CaLaTaO (CLTO) phosphors are synthesized by a high temperature solid phase method. Structural characterization confirms the successful incorporation of Nd and Yb ions into the CLTO host lattice. The photoluminescence excitation (PLE) spectra and photoluminescence (PL) spectra of CLTO:Nd and CLTO:Nd/Yb are investigated in detail.

Peripheral blood ABCG1 gene DNA methylation: mediating the relationship between dietary intake of methyl donor nutrients and stroke risk.

Dysregulation of methyl donor nutrients interferes with DNA methylation and is associated with neurological diseases. ABCG1 gene regulates cholesterol to HDL-C, maintains lipid homeostasis, and has been linked to both methyl nutrition and neurological risks. The aim was to investigate whether there is an effect of ABCG1 DNA methylation on the relationship between intake of methyl donor nutrients and the risk of stroke occurrence.

Mitochondrial dynamics govern whole-body regeneration through stem cell pluripotency and mitonuclear balance.

Tissue regeneration is a complex process involving large changes in cell proliferation, fate determination, and differentiation. Mitochondrial dynamics and metabolism play a crucial role in development and wound repair, but their function in large-scale regeneration remains poorly understood. Planarians offer an excellent model to investigate this process due to their remarkable regenerative abilities.

An update in recent research on nicotine contamination and nicotine-degrading microorganisms.

Nicotine is a toxic and addictive alkaloid found in tobacco and tobacco products that is harmful to human health and is an environmental pollutant. Nicotine-degrading microorganisms are unique microorganisms with the ability to use nicotine molecules as their sole source of nitrogen and carbon needed for growth. They are capable of degrading nicotine into less toxic or non-toxic metabolites.

Ru-Catalyzed Switchable Reactions of Acrylic Acids with Glyoxylate: Access to Functionalized γ-Butenolides.

We herein report a switchable coupling of acrylic acids with ethyl glyoxylate under ruthenium catalysis enabling the synthesis of diverse functionalized γ-butenolides. The carboxyl-directed vinylic C-H cleavage and dual nucleophilic addition to aldehyde are achieved to deliver hydroxymethylated butanolides under mild and oxidant-free conditions. Alternatively, a controlled and unprecedented tandem C-H cyclization/oxidative homocoupling reaction is realized by using silver salt as the oxidant to generate a range of dimeric butenolides bearing vicinal all-carbon quaternary centers.

Rapid detection of carbapenemase production in using phenotypic tests based on colorimetric microtube assay.

Antibiotic resistance, particularly carbapenem resistance, poses a significant global health threat due to the limited availability of effective antibiotics. Carbapenem-resistant are increasingly recognized for their role in various infections, necessitating rapid and accurate detection methods. This study aimed to evaluate several phenotypic tests, including the Carba NP test (CNPt), Carba NP-direct test (CNPd), and Blue-Carba test (BCT), for their effectiveness in rapidly detecting carbapenemase production in .

Convergent evolution of berberine biosynthesis.

Berberine is an effective antimicrobial and antidiabetic alkaloid, primarily extracted from divergent botanical lineages, specifically (Ranunculales, early-diverging eudicot) and (Sapindales, core eudicot). In comparison with its known pathway in species, its biosynthesis in species remains elusive. Using chromosome-level genome assembly, coexpression matrix, and biochemical assays, we identified six key steps in berberine biosynthesis from , including methylation, hydroxylation, and berberine bridge formation.

Preclinical evaluation of the CD38-targeting engineered toxin body MT-0169 against multiple myeloma.

Despite significant progress in the treatment of multiple myeloma (MM), relapsed/refractory patients urgently require more effective therapies. We here describe the discovery, mechanism of action, and preclinical anti-MM activity of engineered toxin body MT-0169, a next-generation immunotoxin comprising a CD38-specific antibody fragment linked to a de-immunized Shiga-like toxin A subunit (SLTA) payload. We show that specific binding of MT-0169 to CD38 on MM cell lines triggers rapid internalization of SLTA, causing cell death via irreversible ribosome inhibition, protein synthesis blockade, and caspase 3/7 activation.

Association of plasma homocysteine with cardiometabolic multimorbidity: a cross-sectional study in northwest China.

Background: Elevated homocysteine (Hcy) levels have been linked to cardiovascular disease (CVD), but their association with cardiometabolic multimorbidity (CMM) remains uncertain.

Methods: Data from the baseline survey of the China Northwest Cohort-Ningxia Project (CNC-NX) were used to recruit 22,566 participants. Demographic characteristics, lifestyle factors, and laboratory exam results were collected.

['s reinforcing and reducing acupuncture method for post-stroke swallowing dysfunction in the pharyngeal phase: a randomized controlled trial].

Objective: To observe the clinical effect of 's reinforcing and reducing acupuncture method on post-stroke swallowing dysfunction (PSD) in the pharyngeal phase, and explore its biomechanical mechanism.

Methods: A total of 72 patients with PSD in the pharyngeal phase were randomly divided into a ziwu reinforcing and reducing acupuncture group (36 cases, 2 cases dropped out and 1 case was eliminated) and a conventional acupuncture group (36 cases, 1 case dropped out and 1 case was eliminated). The patients in the conventional acupuncture group were treated with conventional acupuncture at Lianquan (CV 23) and bilateral Fengchi (GB 20), Wangu (GB 12), Tongli (HT 5), Zhaohai (KI 6), Hegu (LI 4), Taichong (LR 3).

PcWRKY1 Represses Transcription of Yellow Stripe-Like 3 (PcYSL3) to Negatively Regulate Radial Cadmium Transport in Poplar Stems.

A considerable amount of cadmium (Cd) can accumulate in the bark of poplar stems, but the Cd transport pathway and its underlying molecular mechanisms remain unknown. Here, a Cd radial transport pathway in poplar stems and a previously unrecognized PcWRKY1-Yellow Stripe-Like 3 (PcYSL3) module that regulates Cd transport are identified in Populus × canescens (Aiton) Sm. Cadmiun-nicotianamine (Cd-NA) in xylem vessels in poplar stem-wood is unloaded to adjacent ray parenchyma cells and further radially transported to bark-phloem.

Effects of Mozart-Orff parent-child music therapy among mothers and their preschool children with autism spectrum disorder: A mixed-methods randomised controlled trial.

Background: Autism spectrum disorder (ASD) negatively impacts mental health, particularly in mothers of autistic children who experience heightened stress. Applied behaviour analysis (ABA) and music therapy are recognised interventions for improving ASD symptoms. However, the specific benefits of parent-child music therapy and ABA for autistic children and their mothers remain uncertain.

Visible to infrared wide wavelength range luminescence of SrLaNbO: Yb/Nd/Ho phosphor under 808/980 nm excitation and temperature sensing application.

Here, the SrLaNbO: Yb/Nd/Ho phosphors are prepared through solid-state route. The upconversion (UC)/downshifting (DS) luminescence and temperature performances of all samples are investigated in detail. Under 808 nm excitation, the infrared emission band (∼2000 nm) of Ho ions is obtained.

Feasibility and mechanism of adsorption and bioreduction of hexavalent chromium using Rhodopseudomonas palustris immobilized on multiple materials.

Rhodopseudomonas palustris immobilized on multiple materials was used to invistigate Cr(VI) adsorption and bioreduction. The highest Cr(VI) removal (97.5%) was achieved at 276h under the opitimed conditions of 2.

Performance, mechanism regulation and resource recycling of bacteria-algae symbiosis system for wastewater treatment: A review.

The bacteria-algae synergistic wastewater treatment process not only efficiently eliminates nutrients and absorbs heavy metals, but also utilizes photosynthesis to convert light energy into chemical energy, generating valuable bioresource. The study systematically explores the formation, algal species, and regulatory strategies of the bacterial-algal symbiosis system. It provides a detailed analysis of various interaction mechanisms, with a particular focus on nutrient exchange, signal transduction, and gene transfer.

Discontinuation of immune checkpoint inhibitors in hepatocellular carcinoma: a retrospective cohort study.

Background: The optimal timing to discontinue immune checkpoint inhibitor (ICI) therapy in hepatocellular carcinoma (HCC) patients with clinical benefits remains unclear. This study aimed to assess the outcomes of HCC patients after ICI discontinuation.

Methods: Patients with HCC were retrospectively screened and those discontinued ICI therapy in the absence of progressive disease (PD) were included.

Association between dietary antioxidant indices and hypertension among Chinese adults.

The effect of dietary antioxidants on blood pressure (BP) regulation and hypertension risk remains largely unknown. This study aimed to comprehensively assess the impacts of dietary antioxidants on systolic BP (SBP), diastolic BP (DBP), mean arterial pressure (MAP), and pulse pressure (PP), and hypertension risk among Chinese adults. The cross-sectional study assessed data from 12,046 Chinese adults, evaluating dietary antioxidant quality scores (DAQS) and total antioxidant capacity (DTAC) via a validated food frequency questionnaire.

Degradation effects and mechanisms of on ethanol.

Acute heavy drinking can lead to a rapid increase in blood ethanol concentration, resulting in dizziness, liver damage, and other adverse effects. Although lactic acid bacteria possess the ability to degrade ethanol, the mechanisms remain unclear. For the first time, our study revealed that DACN611, derived from traditional Chinese fermented yogurt, exhibited superior ethanol degradation capability, achieving a 90.

Effect of salt substitution on fracture-a secondary analysis of the Salt Substitute and Stroke Study (SSaSS).

Background: Associations of dietary sodium and potassium intake with fracture risk are inconsistent and the effects of salt substitute on fracture incidence are unknown. We assessed the effect of salt substitute compared to regular salt intake on fracture incidence using data from the Salt Substitute and Stroke Study (SSaSS).

Methods: SSaSS was a cluster-randomized controlled trial conducted in 600 villages in northern China.

Impact of vitamins A, D, and homocysteine on cardiometabolic multimorbidity in Northwest China.

Objective: To investigate the impact of vitamin A (VA), vitamin D (VD), and homocysteine (Hcy) on cardiometabolic multimorbidity (CMM).

Methods: This study is a cross-sectional study conducted in Ningxia Province, China. A total of 5000 participants aged 25-74 were recruited and divided into two groups based on the definition of cardiometabolic multimorbidity: the CMM group and the Non CMM group.