Publications by authors named "Yuhang Luo"

Buffalo hunnivirus (BufHuV) is an important pathogen, which can cause diarrhea in water buffaloes, and as yet, there are no vaccines and drugs for its prevention and control. Here we studied the immunogenicity and predicted the three-dimensional structure of the BufHuV VP1 protein, in order to establish a rapid and efficient serological assay for detection of its antibodies in the host. The N-terminal truncated gene, consisting of amino acids 5-117, was selected and cloned into the prokaryotic expression vector pET-32a (+) to obtain the recombinant plasmid, pET-32a-BufHuV-VP1-1.

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Article Synopsis
  • - Bovine enterovirus (BEV) infections can lead to various symptoms in cattle, impacting their respiratory, gastrointestinal, and reproductive health, and causing significant economic losses in the bovine industry globally.
  • - The study utilized a method called reverse transcription-polymerase chain reaction to create a chimeric protein from BEV's VP1 and VP2 genes, which was then used to immunize mice and test for immune response.
  • - Results indicated that this immunization reduced virus shedding and tissue damage, improving overall health in the infected animals, which supports the potential for developing a vaccine against BEV.
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Unlabelled: Human norovirus (HuNoV) is recognized as the leading causative agent of foodborne outbreaks of epidemic gastroenteritis. Consequently, there is a high demand for developing point-of-care testing for HuNoV. We developed an origami microfluidic device that facilitates rapid detection of murine norovirus 1 (MNV-1), a surrogate for HuNoV, encompassing the entire process from sample preparation to result visualization.

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Astroviruses are single-stranded, positive-sense RNA viruses capable of infecting humans as well as a wide range of mammalian and avian species, with a length of approximately 6.6-7.7 kb.

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Low molecular weight chitosan selenium nanoparticles (LCS-SeNPs), a biologically active compound derived from selenium polysaccharides, have demonstrated potential in addressing obesity. However, the mechanism through which LCS-SeNPs alleviate high-fat diet (HFD)-induced non-alcoholic fatty liver disease (NAFLD) remains unclear. Our results elucidated that LCS-SeNPs significantly inhibited fat accumulation and markedly improved the intestinal barrier by increasing mucus secretion from goblet cells.

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The emerging prefabricated decoration (PD) sector in China offers a compelling avenue for revolutionizing the construction industry, aligning with the imperatives of sustainability and industrialization. However, the outlook for the diffusion of PD in China's construction industry is less than promising. The development of PD is closely related to its value creation and the intricate interplay of stakeholder strategies and their ramifications on PD's value co-creation necessitates thorough exploration.

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  • * A new strain of MRV called GXLZ2301 was found in China and is different because it has bits of genes from humans, cows, and pigs.
  • * This new virus can cause serious damage to important organs in animals and may be a risk to human health, so it's important to keep an eye on it.
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  • Goose astroviruses (GAstVs) lead to severe gout in goslings, causing significant economic losses in China's goose farming sector, especially following outbreaks in 2023.
  • Researchers isolated two GAstV strains (GXNN and GDCS) from affected farms, both identified as GAstV-2 subtypes, with distinct mutations found in their capsid proteins.
  • Infected 1-day-old goslings exhibited serious symptoms like visceral gout, enlarged spleens, and high mortality rates, which were 80% for GXNN and 60% for GDCS, highlighting the urgency to understand GAstVs' evolutionary trends and epidemic risks in the region.
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  • Fatigue is when you feel super tired and have low energy, making it hard to do things, especially after exercising.
  • Central fatigue happens when your brain struggles to tell your muscles to work, often during moderate or light exercise, but can also occur with intense workouts.
  • To help athletes perform better and avoid feeling worn out, it's important to find ways to prevent central fatigue and use safe methods to improve their performance.
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Porcine epidemic diarrhea (PED) is a highly contagious intestinal infection primarily affecting pigs. It is caused by the porcine epidemic diarrhea virus (PEDV). PEDV targets the villus tissue cells in the small intestine and mesenteric lymph nodes, resulting in shortened intestinal villi and, in extreme cases, causing necrosis of the intestinal lining.

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Interferons (IFNs) are antiviral cytokines that defend against viral infections by inducing the expression of interferon-stimulated genes (ISGs). Interferon-inducible transmembrane proteins (IFITMs) 1, 2, and 3 are crucial ISG products and members of the CD225 protein family. Compelling evidence shows that IFITMs restrict the infection of many unrelated viruses by inhibiting the virus-cell membrane fusion at the virus entry step via the modulation of lipid composition and membrane properties.

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Expanding the network of connected and resilient protected areas (PAs) for climate change adaptation can help species track suitable climate conditions and safeguard biodiversity. This is often overlooked when expanding PAs and quantifying their benefits, resulting in an underestimate of the benefits of expanding PAs. We expanded PAs through terrestrial mammalian species distribution hotspots, Key Biodiversity Areas (KBAs), and wilderness areas.

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Replacing expensive silver with inexpensive copper for the metallization of silicon wafer solar cells can lead to significant reductions in material costs associated with cell production, but the susceptibility of the Cu material to oxidation remains a challenging issue to solve. In this study, we investigate copper metallization of Indium Tin Oxide surfaces to define copper grid electrodes for heterojunction cells. We propose a novel laser-induced selective metallization (LISM) method to fabricate large-scale copper electrodes for heterojunction solar cells at low cost.

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It is unclear what caused the Bangong Nujiang suture zone in the central Tibetan plateau to rise from less than 2 km in early Cenozoic to more than 4 km at present. The zircon U-Pb ages and trace elements of samples from the Niubao Formation in the Paleogene of the Nima basin were analyzed and tested. Combined with the isostasy theory, the surface uplift height of the Nima Basin during the Cenozoic period was calculated.

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The dynamics of polymer nanocomposites varies depending on the physics and chemistry at the polymer-nanoparticle interface. The physical aging of the nanocomposites is accelerated or retarded based on interfacial interactions and the state of polymer adsorption at the interfaces. In this study, we investigated the aging kinetics of silica-polystyrene nanocomposites using differential scanning calorimetry, focusing on the effect of local conformations of chains adsorbed on the nanofiller surface.

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Natural rubber (NR) is extensively utilized in numerous industries, such as aerospace, military, and transportation, because of its exceptional elasticity and all-around mechanical qualities. However, commercial NR made using various techniques typically has distinct mechanical characteristics. For instance, whole field latex rubber (SCR-WF) cured with accelerator 2-Mercaptobenzothiazole exhibits poor mechanical properties.

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Riboswitches are regulatory elements found in the untranslated regions (UTRs) of certain mRNA molecules. They typically comprise two distinct domains: an aptamer domain that can bind to specific small molecules, and an expression platform that controls gene expression. Riboswitches work by undergoing a conformational change upon binding to their specific ligand, thus activating or repressing the genes downstream.

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Tri-training expands the training set by adding pseudo-labels to unlabeled data, which effectively improves the generalization ability of the classifier, but it is easy to mislabel unlabeled data into training noise, which damages the learning efficiency of the classifier, and the explicit decision mechanism tends to make the training noise degrade the accuracy of the classification model in the prediction stage. This study proposes the Tri-training algorithm for adaptive nearest neighbor density editing and cross-entropy evaluation (TTADEC), which is used to reduce the training noise formed during the classifier iteration and to solve the problem of inaccurate prediction by explicit decision mechanism. First, the TTADEC algorithm uses the nearest neighbor editing to label high-confidence samples.

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Bovine enterovirus (BEV) is a highly infectious pathogen that may cause respiratory and gastrointestinal disease outbreaks in cattle. This study aimed to investigate the prevalence and genetic characteristics of BEVs in Guangxi Province, China. A total of 1,168 fecal samples from 97 different bovine farms were collected between October 2021 and July 2022 in Guangxi Province, China.

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This study developed a novel, ultrasensitive sandwich-type electrochemical immunosensor for detecting the porcine epidemic diarrhea virus (PEDV). By electrochemical co-deposition of graphene and Prussian blue, a Prussian blue-reduced graphene oxide-modified glassy carbon electrode was made, further modified with PEDV-monoclonal antibodies (mAbs) to create a new PEDV immunosensor using the double antibody sandwich technique. The electrochemical characteristics of several modified electrodes were investigated using cyclic voltammetry (CV).

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Purpose: Mucinous histology is generally considered as a risk factor of prognosis in stage II colon cancer, but there is no appropriate model for prognostic evaluation and treatment decision in patients with stage II colon mucinous adenocarcinoma (C-MAC) Thus, it is urgent to develop a comprehensive, individualized evaluation tool to reflect the heterogeneity of stage II C-MAC.

Methods: Patients with stage II C-MAC who underwent surgical treatment in the Surveillance, Epidemiology, and End Results Program were enrolled and randomly divided into training cohort (70%) and internal validation cohort (30%). Prognostic predictors which were determined by univariate and multivariate analysis in the training cohort were included in the nomogram.

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Constructing an ecological security pattern by matching the supply and demand of ecosystem services is of great significance for Shenzhen, China. To determine the strategic positioning of sustainable development pioneers and benchmarks for human well-being in a pilot demonstration area of socialism with Chinese characteristics, we selected water yield, carbon sequestration, and recreational services to identifiy the integrated supply and demand status of ecosystem services in Shenzhen using the InVEST model, ecological supply-demand ratio, cell phone signalling data analysis, and circuit theory. An ecological security pattern based on the matching of supply and demand of ecosystem services was constructed.

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Background: Icariin presents protective effect in several kidney diseases. However, the role of icariin in contrast-induced acute kidney injury (CIAKI) is still unclear. This study aimed to investigate the effect of icariin in CIAKI, as well as exploring the underlying mechanism from the aspect of interaction between protein-coding genes and non-coding RNAs.

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In recent years, hunniviruses have been reported in a variety of animal species from many countries. Here, hunnivirus was detected in fecal samples from water buffaloes and named as BufHuV-GX-2106. The samples were inoculated into cultures of MDBK cells supplemented with TPCK trypsin and the BufHuV-GX-2106 strain was stably passaged and replicated.

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Background: A-Kinase interacting protein 1 (AKIP1) relates to gastric cancer growth, metastasis, and prognosis, while its regulation on gastric cancer invasion and stemness under hypoxia microenvironment is not reported. Therefore, this study aimed to explore this topic to uncover AKIP1's role in gastric cancer under hypoxia.

Methods: Gastric cancer cell lines AGS and MKN45 were cultured under hypoxia condition, then transfected with AKIP1 or negative control (NC) overexpression plasmid or AKIP1 or NC knockdown plasmid.

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