Publications by authors named "Yugoviandi P Mamahit"
To uncover how cells distinguish between misfolded and correctly-folded glycoproteins, homogeneous misfolded glycoproteins are needed as a probe for analysis of their structure and chemical characteristic nature. In this study, we have synthesized misfolded glycosyl interleukin-8 (IL-8) by combining E. coli expression and chemical synthesis to improve the synthetic efficiency.
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- Sequential trimming of mannose from glycoproteins plays a crucial role in their degradation within the endoplasmic reticulum, helping eliminate improperly folded proteins.
- The study confirms that EDEM2, EDEM3, and EDEM1 are the key enzymes involved in this trimming process, with EDEM3 being significant for processing from M8B to further forms.
- Additionally, the Golgi localization of MAN1B indicates that no other mannosidases are needed for the degradation pathway of misfolded glycoproteins.