Impact assessment on the economy, society, resource, and environment in the water-receiving cities of the Middle Route Project of South-to-North Water Diversion.

The Middle Route Project of South-to-North Water Diversion (MRPSNWD) is a major strategic project to alleviate the serious shortage of water resources in northern China, and it benefited 14 large- and medium-sized cities in Henan, Hebei, Beijing, and Tianjin. Most of the predecessors used qualitative analysis to study the impact of the MRPSNWD on the water-receiving cities (WRCs), and they only considered one aspect of economy, society, resources, and environment, which could not fully reflect the impact of the MRPSNWD on the water-receiving cities. Therefore, based on the data of economic and social resources and environment of the water-receiving cities in the MRPSNWD from 2010 to 2019, an evaluation model based on entropy weight-analytic hierarchy process (AHP) fuzzy comprehensive evaluation method was constructed to account for the impacts on the economy-society-resources-environment of the water-receiving cities after the operation of the MRPSNWD and scientifically evaluated the comprehensive benefits of the MRPSNWD.

Microwave-assisted iron oxide process for efficient removal of tetracycline.

Excess tetracycline produced by livestock and poultry breeding industry not only pollute the environment, but also enter the human body with the spread of the food chain, which will produce drug-resistant genes and threaten human health. In this paper, the adsoption of tetracycline from wastewater using iron oxide under microwave oxidation condition was explored. The effect of initial pollutant concentrations, the dosage of iron oxide, pH value, and temperature on the hybrid treatment process was investigated.

Concurrent Disruption of Genetic Interference and Increase of Genetic Recombination Frequency in Hybrid Rice Using CRISPR/Cas9.

Manipulation of the distribution and frequency of meiotic recombination events to increase genetic diversity and disrupting genetic interference are long-standing goals in crop breeding. However, attenuation of genetic interference is usually accompanied by a reduction in recombination frequency and subsequent loss of plant fertility. In the present study, we generated null mutants of the gene, which encodes the central component of the meiotic synaptonemal complex (SC), in a hybrid rice using CRISPR/Cas9.

Erratum to: Rapid generation of genetic diversity by multiplex CRISPR/Cas9 genome editing in rice.

The same figure was misused for the PCR/RE assay results of Gn1a and GW2 fragments in Figure 3, and the arrows in the graphicsal result of GW2 were not on the tape. The corrected Figure 3 is as follows.

Clonal seeds from hybrid rice by simultaneous genome engineering of meiosis and fertilization genes.

Heterosis, or hybrid vigor, is exploited by breeders to produce elite high-yielding crop lines, but beneficial phenotypes are lost in subsequent generations owing to genetic segregation. Clonal propagation through seeds would enable self-propagation of F hybrids. Here we report a strategy to enable clonal reproduction of F rice hybrids through seeds.

A simple and efficient method for CRISPR/Cas9-induced mutant screening.

The clustered regularly interspaced short palindromic repeats (CRISPR)/CRISPR-associated protein 9 (Cas9) system provides a technological breakthrough in mutant generation. Several methods such as the polymerase chain reaction (PCR)/restriction enzyme (RE) assay, T7 endonuclease I (T7EI) assay, Surveyor nuclease assay, PAGE-based genotyping assay, and high-resolution melting (HRM) analysis-based assay have been developed for screening CRISPR/Cas9-induced mutants. However, these methods are time- and labour-intensive and may also be sequence-limited or require very expensive equipment.

Rapid generation of genetic diversity by multiplex CRISPR/Cas9 genome editing in rice.

The clustered regularly interspaced short palindromic repeats (CRISPR)-associated endonuclease 9 (CRISPR/Cas9) system has emerged as a promising technology for specific genome editing in many species. Here we constructed one vector targeting eight agronomic genes in rice using the CRISPR/Cas9 multiplex genome editing system. By subsequent genetic transformation and DNA sequencing, we found that the eight target genes have high mutation efficiencies in the T generation.

Influence of Parent Artery Segmentation and Boundary Conditions on Hemodynamic Characteristics of Intracranial Aneurysms.

Purpose: The purpose of this study is to explore the influence of segmentation of the upstream and downstream parent artery and hemodynamic boundary conditions (BCs) on the evaluated hemodynamic factors for the computational fluid dynamics (CFD) analysis of intracranial aneurysms.

Materials And Methods: Three dimensional patient-specific aneurysm models were analyzed by applying various combinations of inlet and outlet BCs. Hemodynamic factors such as velocity pattern, streamline, wall shear stress, and oscillatory shear index at the systolic time were visualized and compared among the different cases.