Photo-crosslinked bioactive BG/BMSCs@GelMA hydrogels for bone-defect repairs.

The clinical treatments of bone defects remain a challenge. Hydrogels containing bone marrow mesenchymal stem cells (BMSCs) are extensively used to bone regeneration because of excellent biocompatibility and hydrophilicity. However, the insufficient osteo-induction capacity of the BMSC-loaded hydrogels limits their clinical applications.

Hydrophilic surface-modified 3D printed flexible scaffolds with high ceramic particle concentrations for immunopolarization-regulation and bone regeneration.

Bioceramic scaffolds used in bone tissue engineering suffer from a low concentration of ceramic particles (<50 wt%), because the high concentration of ceramic particles increases the brittleness of the composite. 3D printed flexible PCL/HA scaffolds with high ceramic particle concentrations (84 wt%) were successfully fabricated in this study. However, the hydrophobicity of PCL weakens the composite scaffold hydrophilicity, which may limit the osteogenic ability to some extent.

[Comparative analysis of clinical features of primary and secondary laryngeal tuberculosis].

The aim of this study was to analyze and compare the clinical pattern, diagnosis and treatment in primary and secondary laryngeal and pharyngeal TB. A retrospective analysis was carried out on 103 patients diagnosed laryngeal tuberculosis in our department. Characteristics of demography, life-style, clinical features, diagnosis and treatment were obtained from medical case records.

Unique WSPA protein from terrestrial macroscopic cyanobacteria can confer resistance to osmotic stress in transgenic plants.

The terrestrial macroscopic cyanobacterium Nostoc commune exhibits remarkable resistance to desiccation stress. This species synthesizes abundant acidic water stress protein (WSPA) in cells upon desiccation and secretes it into the extracellular polysaccharide sheath upon rehydration. However, our knowledge about its cellular role in stress resistance is still rather limited.

Quality evaluation of the edible blue-green alga Nostoc flagelliforme using a chlorophyll fluorescence parameter and several biochemical markers.

Nostoc flagelliforme is an edible blue-green alga with herbal and dietary values. Due to the diminishing supply of natural N. flagelliforme and the large investment on the development of its cultivation technology, it is anticipated that artificially cultured N.

Resurfacing partial nose defects with a retroauricular skin/cartilage free flap.

Background: The retroauricular skin has always received much attention by plastic surgeons for use in nose reconstruction because of its rich vascularity. Anatomically, the skin of this area lies hidden behind the ear and is very similar to that of the face. More importantly, the retroauricular skin flap along with the cartilage can be used to repair defective cartilage of the nose.

A simple, minimally invasive method for creation of the superior palpebral fold in Asians with the modified continuous buried tarsal stitch: a joint assessment from Toronto, Ontario, Canada, and Chengdu, China.

A simple, minimally invasive stitch method to construct the superior palpebral fold in Asian upper eyelids is described. With the use of a clear 6-0 monofilament polypropylene suture and a continuous stitch and by securing multiple consecutive transcutaneous intratarsal fixations, we were able to produce a consistent, natural result with high success rates and no noticeable scarring in 1648 cases over a 9-year period at 2 surgical centers in Toronto, Ontario, Canada, and Chengdu, China.

Wedge-shaped excision and modified vertical mattress suture fully buried in a multilayered and tensioned wound closure.

Background: A successful deep multilayered wound suture should provide a firm tension-relieving closure, good wound-edge eversion, hemostasis, and minimal intradermal extraneous materials. However, this is not always achieved with a single standard technique. The authors describe their modification of a wound closure method that can rapidly and reliably achieve these results.

Repair of faciocervical scars by expanded deltopectoral flap.

Objective: To explore the approach to repair faciocervical scars.

Methods: Eighty-six patients with large faciocervical scars were repaired using the expanded deltopectoral flaps. After expansion, the flaps were transpositioned to repair faciocervical scars.

[The design of the expanded flap for the repair of the temporal and cheek area].

Objective: To explore the design of an expanded flap at the temporal and cheek area.

Methods: The expanded flap was used for the repair of 619 temporal and cheek defects secondary to scar, nevus or hemangioma excision. In the frontal area, the rotational flap was usually used.

Local hatchet flap for facial skin defects reconstruction in special areas.

Background: The local hatchet flap has been used to reconstruct skin defects of the face with satisfactory results. Cosmetically, it has advantages over other local flaps when skin defects are located in special areas such as the eyelid.

Objective: The purpose of this study was to examine the application of local hatchet flaps for facial defect reconstruction, especially in cases where reconstruction is classically difficult for cosmetic and functional rehabilitation.

[Management of severe postburn scar contracture in the lower extremities].

Objective: To summarize the clinical experience in the management of severe postburn scar contracture in the lower extremities.

Methods: The scars in popliteal fossa and ankle joint were excised. Traction and skin grafting were employed in the management of contracture deformity in these areas.

[Experimental study of tissue engineered blood vessel with vascular endothelial cell and vascular smooth muscle cell].

Objective: To investigate the feasibility to seed vascular endothelial cell(VEC) and vascular smooth muscle cell (VSMC) into tissue engineered blood vessel scaffold material.

Methods: 1. A blood vessel scaffold with a combined polymer was designed, which mainly is composed of rabbit VSMC and collagen with reinforcement by a non-spinning fabric mesh made of polyglycolic acid (PGA).

[Fabrication of a blood vessel scaffold with a combined polymer for tissue engineering].

Objective: To investigate the possibility to fabricate a blood vessel scaffold with a combined polymer for tissue engineering.

Methods: A blood vessel scaffold was designed with a combined polymer composed of rabbit vascular smooth muscle cells(VSMCs), collagen and a non-spinning fabric mesh of polyglycolic acid (PGA). VSMCs were implanted into collagen gel and their growth was observed.

[The expanded distant skin flap and its application].

Objective: To summarize and present the experiences and results of the expanded distant skin flaps used in plastic and reconstructive operations.

Methods: 102 patients who had undergone expanded distant skin flap transfer were reviewed and followed up.

Results: Good results were obtained.

[Preparation of compound biodegradable matrices and growth of vascular endothelial cell on them].

Objective: To prepare the compound biodegradable matrices, polyglycolic acid (PGA), polylactic acid (PLA) mesh and poly-beta-hydroxybutyrate(PHB) which precoated with collagen, and to observe the growth and differentiation of bovine vascular endothelial cells on these scaffolds.

Methods: By enzymatic digestion methods, bovine vascular endothelial cell (VEC) were isolated from calf thoracic aorta, then cultured and purified. PGA, PLA, PHB meshes were dipped into cross-linked type I collagen solution, dried under vacuum frozen condition.

[Computer-aided tissue expansion 3D profilometry and surgery planning system].

Objective: To develop and evaluate the computer-aided system in measurement of expanded skin and preoperative planning.

Methods: Stereophotogrammetric technique was used to gain the 3D image-pairs, from which the contours of the expanded sites were restored. The 3D surface data were provided to the specially developed "computer-aided tissue expansion 3D profilometry and surgery planning system", to calculate the expansion area and help the preoperative design.

[Reconstruction of partial nose defects with retroauricular free flap transplantation].

Objective: To present the operation for nose defect reconstruction using a retroauricular free flap and the auricle cartilage.

Methods: Based on the anatomy and blood supply of the retroauricular region, a flap was designed and harvested with the posterior auricular vessels as its pedicle. The flap was used to repair nose defects in five patients.

[Rhinoplasty of unilateral cleft lip].

Objective: To investigate the technique for unilateral cleft lip nasal deformities.

Methods: Through the traditional columella margin incision, the alar cartilage and the lateral nasal cartilage were exposed. After the mucosa between the two cartilages was divided, a mucosa-cartilage flap was developed and anchored, moving the lateral crus upwardly and medially, correcting the nasal deformity.