Function of Gene Response to Infection and LPS Stimulation in the Half-Smooth Tongue Sole ().

Lysosome-associated membrane glycoproteins (LAMPs), including lysosomal membrane protein 1 (Lamp1) and lysosomal membrane protein 2 (Lamp2), are involved in phagocytosis, chaperone-mediated autophagy (CMA), and other pathways that interact with lysosomal activity. However, the role of Lamp2 in teleosts has not been clarified. In this study, we investigated the functions of genes during infection.

Mechanisms of NO production in salinity-adapted partial nitritation systems for high-ammonia wastewater treatment.

Partial Nitritation/Anammox (PN/A) can achieve green, economical, and efficient biological nitrogen removal; however, the PN process contributes significantly to nitrous oxide (NO, the third most important greenhouse gas) emissions. Balancing the stability of PN systems while reducing NO emissions, particularly under varying salinity conditions, is a key challenge in applying PN/A for high-salinity and high-ammonia wastewater treatment. This study explored the long-term effects of salinity on PN performance and NO emissions in PN systems treating high-ammonia wastewater.

Isolation, identification and the pathogenicity characterization of Pseudomonas putida 1C3 and its activation on immune responses in Japanese flounder (Paralichthys olivaceus).

The outbreak of mass mortality of Japanese flounder occurred in an aquaculture farm in Hebei province of China. This study isolated and identified Pseudomonas putida as the dominant bacterium from diseased Japanese flounder (Paralichthys olivaceus) based on morphological, physiological, biochemical characteristics, 16S rRNA gene sequencing, and whole-genome sequencing. Pathogenicity assessment, histopathological analysis, and host immune response were investigated.

Evaluation of nitrous oxide reduction in solid carbon source-driven counter-diffusional biofilm denitrification system.

Solid carbon-driven biofilm system can provide sufficient carbon source for denitrification, while its counter-diffusional structure could inevitably induce the delayed carbon-nitrogen contact and electron transport, further affecting carbon footprints mainly contributed by nitrous oxide (NO) at wastewater treatment plants (WWTPs). However, the detailed understanding of NO dynamics during solid-phase denitrification (SPD) has not been disclosed. In this work, a fixed bed bioreactor driven by polycaprolactone (PCL) was constructed and operated over 180 days, achieving 97 %-99 % of total nitrogen (TN) removal efficiency.

Implications of comprehensive geriatric assessment and Traditional Chinese Medicine constitution types for integrative geriatric care.

Background: Complementary medicine may enhance conventional geriatric care, but clinical guidance for incorporating Traditional Chinese Medicine (TCM) therapies remains limited. This study aimed to investigate the link between TCM body constitutions and comprehensive geriatric assessment scores in older adults.

Method: This prospective observational study recruited 100 participants aged 65 and older between January 2020 and December 2021.

Synchronously Mature Intersex Japanese Flounder (): A Rare Case.

Japanese flounder is usually gonochoristic, with gonads that are either testes or ovaries. Here, we report an unusual case of hermaphroditism in Japanese flounder captured from the Bohai Sea. In the intersex flounder, the membrane of the upper ovary was closely connected to the abdominal muscles and internal organs, and the eggs filled the entire abdomen.

Effect, Fate and Remediation of Pharmaceuticals and Personal Care Products (PPCPs) during Anaerobic Sludge Treatment: A Review.

Biomass energy recovery from sewage sludge through anaerobic treatment is vital for environmental sustainability and a circular economy. However, large amounts of pharmaceutical and personal care products (PPCPs) remain in sludge, and their interactions with microbes and enzymes would affect resource recovery. This article reviews the effects and mechanisms of PPCPs on anaerobic sludge treatment.

Evaluation of Female Recipient Infertility and Donor Spermatogonial Purification for Germ Cell Transplantation in .

Since the advent of germ cell transplantation (GCT), it has been widely used in shortening the fish breeding cycle, sex-controlled breeding and the protection of rare and endangered fish. In this study, the effectiveness of female sterile recipient preparation and donor stem cell isolation and purification were comprehensively evaluated for spermatogonial stem cell transplantation (SSCT) in . The best way to prepare sterile recipients was found to be giving three-year-old fish four intraovarian injections of busulfan (20 mg/kg body weight) combined with exposure to a high temperature (28 °C) after the spawning season compared with the two other ways, which induced apoptosis of most of the endogenous germ cells, resulting in shrinkage of the spawning plate and enlargement of the ovarian lumen.

Detecting endolymphatic hydrops in patients with cranial nerve VIII schwannoma using hydrops MR examination.

Purpose: This study utilized Hydrops MRI in patients with cranial nerve (CN) VIII schwannoma to assess the concomitance with endolymphatic hydrops (EH), aiming to elucidate the mechanism of hydrops formation in these patients.

Methods: Twenty-six patients diagnosed as CN VIII schwannoma including vestibular schwannoma (VS) in 24 and intracochlear schwannoma (ICS) in 2 were enrolled. Fifteen patients received radiosurgery and 11 patients opted for a wait-and-scan approach.

The global status of nursing research on hemodialysis: A bibliometric and visualized analysis.

Background: Hemodialysis (HD)-related nursing research has been growing over the past 2 decades, providing critical insights into improving patient care and outcomes. This study aimed to visualize the hotspots and emerging frontiers in HD-related nursing research, offering valuable references for future studies.

Methods: A bibliometric analysis was conducted on publications related to HD nursing research from the Web of Science Core Collection database, spanning the years 2002 to 2023, and the characteristics of literature such as authors, co-cited authors, countries, research institutions, journal distribution, keywords, and cited literature were visually analyzed using CiteSpace and VOSviewer.

Interaction of poly dimethyl diallyl ammonium chloride with sludge components: Anaerobic digestion performance and adaptive changes of anaerobic microbes.

The wide utilization of poly dimethyl diallyl ammonium chloride (polyDADMAC) in industrial conditions leads to its accumulation in waste activated sludge (WAS), thereby affecting subsequent WAS treatment processes. This work investigated the interaction between polyDADMAC and WAS components from the perspective of anaerobic digestion (AD) performance and anaerobes adaptability variation. The results showed that polyDADMAC decreased the content of biodegradable organic substrates (i.

Hydrocyclone combines with alkali-thermal pretreatment to enhance short-chain fatty acids production from anaerobic fermentation of waste activated sludge.

The production of short-chain fatty acids (SCFAs) through anaerobic fermentation of waste activated sludge (WAS) is commonly constrained by limited substrate availability, particularly for WAS with low organic content. Combining the hydrocyclone (HC) selection with alkali-thermal (AT) pretreatment is a promising solution to address this limitation. The results indicated that HC selection modified the sludge properties by enhancing the ratio of mixed liquid volatile suspended solids (MLVSS)/mixed liquid suspended solids (MLSS) by 19.

Biodegradable microplastics aggravate greenhouse gas emissions from urban lake sediments more severely than conventional microplastics.

Freshwater ecosystems, such as urban lake sediments, have been identified as important sources of greenhouse gases (GHGs) to the atmosphere, as well as persistent sinks for ubiquitous microplastics due to the high population density and frequent anthropogenic activity. The potential impacts of microplastics on GHG production, however, remain underexplored. In this study, four types of common biodegradable microplastics (BMPs) versus four conventional non-biodegradable microplastics (NBMPs) were artificially exposed to urban lake sediments to investigate the responses of nitrous oxide (NO) and methane (CH) production, and make a comparison regarding how the biodegradability of microplastics affected GHG emissions.

Spot sign score is associated with hematoma expansion and longer hospital stay but not functional outcomes in primary intracerebral hemorrhage survivors.

Purpose: The computed tomography angiography (CTA) spot sign is a validated predictor of 30-day mortality in intracerebral hemorrhage (ICH). However, its role in predicting unfavorable functional outcomes remains unclear. This study explores the frequency of the spot sign and its association with functional outcomes, hematoma expansion, and length of hospital stay among survivors of ICH.

Prognostic implications of distinctive imaging characteristics in primary intracranial germ cell tumors: A retrospective analysis.

Purpose: Primary central nervous system (CNS) germ cell tumors (GCTs) are rare brain tumors that encompass two subtypes: germinomas and non-germinomatous germ cell tumors (NGGCTs), NGGCTs have less favorable outcome and require multi-modality treatment. Biopsy is recommended for disease diagnosis, the specimen may not adequately reflect the entire tumor. This study aimed to assess distinct imaging characteristics to differentiate between GCT subgroups and to identify possible initial image and subgroup features that influence survival.

Development of Rapid Detection Methods for f. sp. in Melon Seeds.

Melon ( L.) is a global commercial crop that is sensitive to seed-borne wilt infections caused by f. sp.

Synergistic Effects of MOFs and Noble Metals in Photocatalytic Reactions: Mechanisms and Applications.

Photocatalytic technology can efficiently convert solar energy to chemical energy and this process is considered as one of the green and sustainable technology for practical implementation. In recent years, metal-organic frameworks (MOFs) have attracted widespread attention due to their unique advantages and have been widely applied in the field of photocatalysis. Among them, noble metals have contributed significant advances to the field as effective catalysts in photocatalytic reactions.

Molecular dynamics simulation of the effect of temperature on the conformation of ubiquitin protein.

Content: Ubiquitin, a ubiquitous small protein found in all living organisms, is crucial for tagging proteins earmarked for degradation and holds pivotal importance in biomedicine. Protein functionality is intricately linked to its structure. To comprehend the impact of diverse temperatures on ubiquitin protein structure, our study delved into the energy landscape, hydrogen bonding, and overall structural stability of ubiquitin protein at varying temperatures.

Efficient electrosynthesis of HO from air for sulfide control in sewers.

Electrochemically in-situ generation of oxygen and caustic soda is promising for sulfide management while suffers from scaling, poor inactivating capacity, hydrogen release and ammonia escape. In this study, the four-compartment electrochemical cell efficiently captured oxygen molecules from the air chamber to produce HO without generating toxic by-products. Meanwhile, the catalyst layer surface of PTFE/CB-GDE maintained a relatively balanced gas-liquid micro-environment, enabling the formation of enduring solid-liquid-gas interfaces for efficient HO electrosynthesis.

Using Artificial Intelligence to Interpret Clinical Flow Cytometry Datasets for Automated Disease Diagnosis and/or Monitoring.

Flow cytometry (FC) is routinely used for hematological disease diagnosis and monitoring. Advancement in this technology allows us to measure an increasing number of markers simultaneously, generating complex high-dimensional datasets. However, current analytic software and methods rely on experienced analysts to perform labor-intensive manual inspection and interpretation on a series of 2-dimensional plots via a complex, sequential gating process.

Imaging Characteristics and Interpretation Strategy for Renal Hyperparathyroidism: A Retrospective 4-Dimensional Computed Tomography Study.

Objective: Parathyroidectomy treats uncontrolled renal hyperparathyroidism (RHPT), requiring identification of all glands. Three types of enhancement are proposed. Type A lesions have higher arterial phase attenuation than the thyroid, type B lesions lack higher arterial phase attenuation but have lower venous phase attenuation, and type C lesions have neither higher arterial phase attenuation nor lower venous phase attenuation than the thyroid.