Rapid detection assays for Bacillus anthracis, Yersinia pestis, and Brucella spp. via triplex-recombinase polymerase amplification.

Background: Bacillus anthracis (B. anthracis), Yersinia pestis (Y. pestis), and Brucella spp.

Alkali burn injury model of meibomian gland dysfunction in mice.

Aim: To establish a stable, short-time, low-cost and reliable murine model of meibomian gland dysfunction (MGD).

Methods: A filter paper sheet soaked in 1.0 mol/L sodium hydroxide (NaOH) solution was used to touch the eyelid margin of C57BL/6J mice for 10s to establish the model.

Rapid and efficient immune response induced by a designed modular cholera toxin B subunit (CTB)-based self-assembling nanoparticle.

Modular self-assembling nanoparticle vaccines, represent a cutting-edge approach in immunology with the potential to revolutionize vaccine design and efficacy. Although many innovative efficient modular self-assembling nanoparticles have been designed for vaccination, the immune activation characteristics underlying such strong protection remain poorly understood, limiting the further expansion of such nanocarrier. Here, we prepared a novel modular nanovaccine, which self-assembled via a pentamer cholera toxin B subunit (CTB) domain and an unnatural trimer domain, presenting S.

Genome sequences of two strains utilized as veterinary vaccines in China.

In this report, we present the complete genome sequences of two strains utilized as veterinary vaccines in China. The sequencing was conducted using a hybrid assembly methodology that combined Illumina short reads and PacBio long reads. This approach provides a high-quality representative sequence for the strains mentioned above.

Rapid Identification of Genus and Species In Silico and On-Site Using Novel Probes with CRISPR/Cas12a.

Human brucellosis caused by is a widespread zoonosis that is prevalent in many countries globally. The high homology between members of the genus and spp. often complicates the determination of disease etiology in patients.

Genome Sequence and Phenotypic Analysis of a Protein Lysis-Negative, Attenuated Anthrax Vaccine Strain.

is a Gram-positive bacterium that causes the zoonotic disease anthrax. Here, we studied the characteristic phenotype and virulence attenuation of the putative No. II vaccine strain, PNO2, which was reportedly introduced from the Pasteur Institute in 1934.

Genome Sequence of Acinetobacter baumannii Strain SHOU-Ab01, Isolated from Chinese Giant Salamander (Andrias davidianus) Liver.

This report describes the complete genome sequence of Acinetobacter baumannii strain SHOU-Ab01, which was isolated from the liver of a Chinese giant salamander (Andrias davidianus). SHOU-Ab01 belonged to sequence type 40 (ST40), and its genome contained a circular chromosome (size, 3,891,862 bp) and two circular plasmids (sizes, 8,571 bp and 5,870 bp).

Rapid Identification of Bacillus anthracis and On-Site Using Novel Single-Nucleotide Polymorphisms.

Bacillus anthracis is a spore-forming bacterium that causes life-threatening infections in animals and humans and has been used as a bioterror agent. Rapid and reliable detection and identification of B. anthracis are of primary interest for both medical and biological threat-surveillance purposes.

A CRISPR/Cas12a-based DNAzyme visualization system for rapid, non-electrically dependent detection of .

As next-generation pathogen detection methods, CRISPR-Cas-based detection methods can perform single-nucleotide polymorphism (SNP) level detection with high sensitivity and good specificity. They do not require any particular equipment, which opens up new possibilities for the accurate detection and identification of In this study, we developed a complete detection system for based on Cas12a. We used two chromosomally located SNP targets and two plasmid targets to identify with high accuracy.

Transcriptome sequencing reveals high-salt diet-induced abnormal liver metabolic pathways in mice.

Background: Although salt plays an important role in maintaining the normal physiological metabolism of the human body, many abnormalities in the liver caused by a high-salt diet, especially with normal pathological results, are not well characterized.

Methods: Eight-week-old female C57BL/6 mice were randomly divided into a normal group and a high salt group. These groups were then fed with normal or sodium-rich chow (containing 6% NaCl) for 6 weeks.

Application of CRISPR/Cas9 System for Plasmid Elimination and Bacterial Killing of Group Strains.

The CRISPR-Cas system has been widely applied in prokaryotic genome editing with its high efficiency and easy operation. We constructed some "scissors plasmids" via using the temperature-sensitive pJOE8999 shuttle plasmid, which carry the different 20nt (N20) guiding the Cas9 nuclease as a scissors to break the target DNA. We successfully used scissors plasmids to eliminate native plasmids from and , and specifically killed .

Obtaining Specific Sequence Tags for and Visually Detecting Them Using the CRISPR-Cas12a System.

Three worldwide historical plague pandemics resulted in millions of deaths. , the etiologic agent of plague, is also a potential bioterrorist weapon. Simple, rapid, and specific detection of is important to prevent and control plague.

SpoVG is Necessary for Sporulation in .

The spore constitutes the infectious form of the bacterium, and sporulation is an important process in the organism's life cycle. Herein, we show that disruption of SpoVG resulted in defective sporulation. Confocal microscopy demonstrated that a Δ mutant could not form an asymmetric septum, the first morphological change observed during sporulation.

Genotyping and population diversity of Bacillus anthracis in China based on MLVA and canSNP analysis.

In pastoral parts of China, anthrax still presents a major risk to livestock and threatens the health of local human populations. Currently, whole-genome-based molecular markers, such as single-nucleotide polymorphisms (SNPs) and variable number tandem repeats (VNTRs), are the most effective tools for genotyping Bacillus anthracis. In this study, 191 isolates were selected to assess the diversity of B.

Disruption of SpoIIID decreases sporulation, increases extracellular proteolytic activity and virulence in Bacillus anthracis.

Endospores are important for maintenance of the B. anthracis lifecycle and necessary for its effective spread between hosts. Our experiments with B.

Construction of a high-efficiency cloning system using the Golden Gate method and I-SceI endonuclease for targeted gene replacement in Bacillus anthracis.

To investigate gene function in Bacillus anthracis, a high-efficiency cloning system is required with an increased rate of allelic exchange. Golden Gate cloning is a molecular cloning strategy allowing researchers to simultaneously and directionally assemble multiple DNA fragments to construct target plasmids using type IIs restriction enzymes and T4 DNA ligase in the same reaction system. Here, a B.

Design and production of conjugate vaccines against . using an O-linked glycosylation system in vivo.

Enteric fever, mainly caused by serovar , remains a common and serious infectious disease worldwide. As yet, there are no licensed vaccines against . .